Summary
The role of cellular immunity in LCM disease in mice has been well established. In order to investigate the cellular immune response in vitro, a quantitative method was developed based upon the 51Cr release following immunologically specific cytotoxic activity of sensitized lymphocytes upon LCM virus-infected and radioactively labeled L cells. The time course of the cellular immune response was determined for the acutely infected adult mouse as well as for the neonatally infected carrier mouse following transfer of lymphocytes from immune syngeneic donors. While lymphocytes from acutely infected mice specifically lysed LCM virus-infected L cells, no such activity was found when using similar cells from carrier mice. Furthermore, no evidence was obtained showing the presence of enhancing antibody in the virus carriers. Dose-response experiments indicated that a direct cell-to-cell interaction may be involved in the in vitro cell-mediated destruction of LCM virus-infected target cells.
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Marker, O., Volkert, M. (1973). In Vitro Measurement of the Time Course of Cellular Immunity to LCM Virus in Mice. In: Lehmann-Grube, F. (eds) Lymphocytic Choriomeningitis Virus and Other Arenaviruses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65681-1_19
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DOI: https://doi.org/10.1007/978-3-642-65681-1_19
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