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Metabolic Events in the Regulation of Cell Reproduction

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Antineoplastic and Immunosuppressive Agents Part I

Abstract

How the processes of growth and division are regulated in cells is one of the most fascinating problems of modern biology. Within the last two decades the research in this area of biology has undergone rapid change. The cell cycle is no longer regarded naively as a period of steady uniform growth between one cell division and the next. Instead, observations have led us to regard the growth and division of cells as a series of ordered chemical changes with precise temporal control mechanisms.

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References

  • Barka, T.: Stimulation of DNA synthesis by isoproterenol in the salivary gland. Exp. cell Res. 39, 355–364 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Baserga, R.,Stein, G.: Nuclear acidic proteins and cell proliferation. Fed. Proc. 30, 1752–1759 (1971).

    PubMed  CAS  Google Scholar 

  • Brent, T.P., Butler, J.A.V., Crathorn, A.A.: Variations in Phosphokinase activities during the cell cycle in synchronous populations of HeLa cells. Nature (Lond.) 207, 176–177 (1965).

    Article  CAS  Google Scholar 

  • Bucher, N.L.R.: Regeneration of mammalian liver. Int. Rev. Cytol. 15, 245–300 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Cameron, I.L., Greulich, R.C.: Evidence for an essentially constant duration of DNA synthesis in renewing epithelia of the adult mouse. J. cell Biol. 18, 31–40 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Cohen, L.S., Studzinski, G.P.: Correlation between cell enlargement and nucleic acid and protein content of HeLa cells in unbalanced growth produced by inhibitors of DNA synthesis. J. cell. Physiol. 69, 331–340 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Conrad, A.H.: Thymidylate synthetase activity in cultured mammalian cells. J. biol. Chem. 246, 1318–1323 (1971).

    PubMed  CAS  Google Scholar 

  • DeCosse, J.J., Gelfant, S.: Noncycling tumor cells: Mitogenic response to antilymphocytic serum. Science 162, 698–699 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Defendi, V., Manson, L.A.: Analysis of the life-cycle in mammalian cells. Nature (Lond.) 198, 359–361 (1963).

    Article  Google Scholar 

  • Friedman, D.L.: DNA polymerase from HeLa cell nuclei: Levels of activity during a synchronized cell cycle. Biochem. biophys. Res. Commun. 39, 100–109 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Friedman, D.L., Mueller, G.C.: A nuclear system for DNA replication from synchronized HeLa cells. Biochim. biophys. Acta (Amst.) 161, 455–468 (1968).

    CAS  Google Scholar 

  • Fox, T.O., Pardee, A.B.: Proteins made in the mammalian cell cycle. J. biol. Chem. 246, 6159–6165 (1971).

    PubMed  CAS  Google Scholar 

  • Galavazi, G., Schenk, H., Bootsma, D.: Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. I. Optimal conditions. Exp. cell Res. 41, 428–437 (1966).

    CAS  Google Scholar 

  • Gamow, E.L, Prescou, D.M.: The cell life cycle during early embryogenesis of the mouse. Exp. cell Res. 59, 117–125 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Gefter, M.L., Russell, R.L.: Role of modifications in tyrosine transfer RNA: A modified base affecting ribosome binding. J. molec. Biol. 39, 145–157 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Gelbard, A.S., Km, J., Perez, A.: Fluctuations in deoxycytidine monophosphate deaminase activity during the cell cycle in synchronous populations of HeLa cells. Biochim. biophys. Acta (Amst.) 182, 564–566 (1969).

    CAS  Google Scholar 

  • Gelfant, S.: Initiation of mitosis in relation to the cell division cycle. Exp. cell Res. 26, 395–403 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Gold, M., Helleiner, C.W.: Deoxyribonucleic acid polymerase in L-cells. I. Properties of the enzyme and its activity in synchronized cell cultures. Biochim. biophys. Acta (Amst.) 80, 193–203 (1964).

    CAS  Google Scholar 

  • Hungerford, D.A., Donnelly, A.J., Nowell, P.C, Beck, S.: The chromosome constitution of a human phenotypic intersex. Amer. J. Human Genet. 11, 215–236 (1959).

    CAS  Google Scholar 

  • Howard, A., Pelc, S.R.: Nuclear incorporation of P82 as demonstrated by autoradiographs. Exp. cell Res. 2, 178–187 (1951).

    Article  CAS  Google Scholar 

  • Howard, E.F., Stubblefield,E.: Low molecular weight nuclear RNA in phytohemagglutinin-treated and untreated human lymphocytes. Exp. cell Res. 70, 460–462 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Jacob, F., Monod, J.: Genetic regulatory mechanisms in the synthesis of proteins. J. molec. Biol. 3, 318–356 (1961).

    Article  PubMed  CAS  Google Scholar 

  • Krr, S., Dubbs, D.R., Pickarski, L. J., Hsu, T.C: Deletion of thymidine kinase activity from L-cells resistant to bromodeoxyuridine. Exp. cell Res. 31, 297–312 (1963).

    Article  Google Scholar 

  • Klevecz, R.R.: Temporal order in mammalian cells. I. The periodic synthesis of lactate dehydrogenase in the cell cycle. J. cell Biol. 43, 207–219 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Levan, A., Hauschka, T.S.: Endomitotic reduplication mechanisms in ascites tumors of the mouse. J. nat. Cancer Inst. 14, 1–43 (1953).

    PubMed  CAS  Google Scholar 

  • Ley, K.D., Tobey, R.A.: Regulation of initiation of DNA synthesis in Chinese hamster cells. II. Induction of DNA synthesis and cell division by isoleucine and glutamine in G 1-arrested cells in suspension culture. J. cell Biol. 47, 453–459 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Liulefield, J. W.: Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145, 709–710 (1964).

    Article  Google Scholar 

  • Melnykovych, G., Bishop, CF., Swayze, M.A.B.: Fluctuation of alkaline phosphatase activity in synchronized heteroploid cell cultures: Effects of prednisolone. J. cell. Physiol. 70, 231–236 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Miller, R.G., Phillips, R.A.: Separation of cells by velocity sedimentation. J. cell. Physiol. 73, 191–202 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Mitchison, J.M.: Enzyme synthesis in synchronous cultures. Science 165, 657–663 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Miuermayer, C, Bosselmann, R., Bremershov, V.: Initiation of DNA synthesis in a system of synchronized L-cells: Rhythmicity of thymidine kinase activity. Euro. J. Biochem. 4, 487–489 (1968).

    Article  Google Scholar 

  • Moore, E.C., Hurlbert, R.B.: Regulation of mammalian deoxyribonucleotide biosynthesis by nucleotides as activators and inhibitors. J. biol. Chem. 241, 4802–4809 (1966).

    PubMed  CAS  Google Scholar 

  • Mueller, G.C.: Biochemical perspectives of the G 1 and S intervals in the replication cycle of animal cells: A study in the control of cell growth. In: The cell cycle and cancer 1, 269–307. New York: Marcel Dekker 1971.

    Google Scholar 

  • Mueller, G.C, Kajiwara, K.: Actinomycin D and p-fluorophenylalanine, inhibitors of nuclear replication in HeLa cells. Biochim. biophys. Acta (Amst.) 119, 557–565 (1966).

    CAS  Google Scholar 

  • Mueller, G.C, Kajiwara, K., Stubblefield, E., Rueckert, R.R.: Molecular events in the reproduction of animal cells. I. The effect of puromycin on the duplication of DNA. Cancer Res. 22, 1084–1090 (1962).

    PubMed  CAS  Google Scholar 

  • Murphree, S.A.: Temporal variation of enzyme activity and adenosine nucleotide pools during the cell cycle of mammalian cells in culture. Ph. D. Thesis. The University of Texas Graduate School of Biomedical Sciences, Houston, Texas, U.S.A. (1972).

    Google Scholar 

  • Murphree, S.A., Stubblefield, E., Moore, E.C: Synchronized mammalian cell cultures. III. Variation of ribonucleotide reductase activity during the replication cycle of Chinese hamster fibroblasts. Exp. cell Res. 58, 118–124 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Nordenskjöld, B.A., Skoog, L., Brown, N.C, Reichard, P.: Deoxyribonucleotide pools and deoxyribonucleic acid synthesis in cultured mouse embryo cells. J. biol. Chem. 245, 5360–5368 (1970).

    PubMed  Google Scholar 

  • Pfeiffer, S.E., Tolmach, L.J.: Inhibition of DNA synthesis in HeLa cells by hydroxyurea. Cancer Res. 27, 124–129 (1967).

    PubMed  CAS  Google Scholar 

  • Prescou, D.M.: Regulation of cell reproduction. Cancer Res. 28, 1815–1820 (1968).

    Google Scholar 

  • Quastler, H., Sherman, F.G.: Cell population kinetics in the intestinal epithelium of the mouse. Exp. cell Res. 17, 420–438 (1959).

    Article  PubMed  CAS  Google Scholar 

  • Rao, P.N., Johnson, R.T.: Mammalian cell fusion: Studies on the regulation of DNA synthesis and mitosis. Nature (Lond.) 225, 159–164 (1970).

    Article  CAS  Google Scholar 

  • Rueckert, R.R., Mueller, C.G.: Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency. Cancer Res. 20, 1584–1591 (1960).

    PubMed  CAS  Google Scholar 

  • Salas, J., Green, H.: Proteins binding to DNA and their relation to growth in cultured mammalian cells. Nature (Lond.) 229, 165–169 (1971).

    CAS  Google Scholar 

  • Sinclair, W.K.: Hydroxyurea: Differential lethal effects on cultured mammalian cells during the cell cycle. Science 150, 1729–1731 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Stubblefield, E.: Synchronization methods for mammalian cell cultures. In: Methods in cell physiology 3, 25–43. New York: Academic Press 1968.

    Google Scholar 

  • Stubblefield, E., Klevecz, R.: Synchronization of Chinese hamster cells by reversal of colcemid inhibition. Exp. cell Res. 40, 660–664 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Stubblefield, E., Klevecz, R., Deaven, L.: Synchronized mammalian cell cultures. I. Cell replication cycle and macromolecular synthesis following brief colcemid arrest of mitosis. J. cell. Physiol. 69, 345–354 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Stubblefield, E., Mueller, G.C: Thymidine kinase activity in synchronized HeLa cell cultures. Biochem. biophys. Res. Commun. 20, 535–538 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Stubblefield, E., Murphree, S.A.: Synchronized mammalian cell cultures. II. Thymidine kinase activity in colcemid synchronized fibroblasts. Exp. cell Res. 48, 652–656 (1967).

    Article  CAS  Google Scholar 

  • Teraslma, T., Tolmach, L.J.: Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp. cell Res. 30, 344–362 (1963).

    Article  Google Scholar 

  • Thompson, L.R., McCarthy, B.J.: Stimulation of nuclear DNA and RNA synthesis by cyto-plasmic extracts in vitro. Biochem. biophys. Res. Commun. 30, 166–172 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Tidwell, T.: The methylation of transfer ribonucleic acid during regeneration of the liver. J. cell Biol. 46, 370–378 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Tidwell, T., Stubblefield, E.: Methylation and synthesis of transfer ribonucleic acid in synchronized Chinese hamster fibroblasts. Exp. cell Ees. 64, 350–354 (1971).

    Article  CAS  Google Scholar 

  • Turner, M.K., Abrams, R., Lieberman, L: Levels of ribonucleotide reductase activity during the division cycle of the L cell. J. biol. Chem. 243, 3725–3728 (1968).

    PubMed  CAS  Google Scholar 

  • Watson, J.D.: The regulation of DNA synthesis in eukaryotes. Advanc. cell Biol. 2, 1–46 (1971).

    CAS  Google Scholar 

  • Whumore, G.F.: Natural and induced synchronous cultures. In Vitro 6, 276–285 (1971).

    Article  Google Scholar 

  • Xeros, N.: Deoxyriboside control and synchronization of mitosis. Nature (Lond.) 194, 682–683 (1962).

    Article  CAS  Google Scholar 

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Stubblefield, E., Murphree, S. (1974). Metabolic Events in the Regulation of Cell Reproduction. In: Sartorelli, A.C., Johns, D.G. (eds) Antineoplastic and Immunosuppressive Agents Part I. Handbuch der experimentellen Pharmakologie / Handbook of Experimental Pharmacology, vol 38 / 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65678-1_9

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  • DOI: https://doi.org/10.1007/978-3-642-65678-1_9

  • Publisher Name: Springer, Berlin, Heidelberg

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