Skip to main content
SpringerLink
Log in

Bioassay of Angiotensin

  • Chapter
Angiotensin

Abstract

This chapter is not intended to be an exhaustive survey of all the bioassay techniques published relating to angiotensin. Instead it is limited to measurement of blood pressure responses, mainly on the rat, and to measurement of isolated smooth muscle contractions. Finally, brief mention is made of another technique, which has not yet been utilized as an assay system, although its sensitivity and specificity are adequate for this purpose. This method involves the measurement of catecholamines released from a perfused adrenal gland. Quantitative extraction of angiotensin from body fluids is discussed in chapter “Measurement of Renin and of Angiotensin (Extraction and Bioassay)” by R. Boucher and J. Genest.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ariens, E. S., De Groot, W. M.: Affinity and intrinsic activity in the theory of competitive inhibition. III. Homologous decamethonium derivatives and succinyl-choline esters. Arch, int. Pharmacodyn. 99, 193 (1954).

    CAS  Google Scholar 

  • Armitage, A. K., Vane, J. R.: A sensitive method for the assay of catecholamines. Brit. J. Pharmacol. 22, 204 (1964).

    PubMed  CAS  Google Scholar 

  • Boucher, R., Biron, P., Genest, J.: Procedure for isolation and determination of human blood angiotensin. Cañad. J. Biochem. 39, 581 (1961).

    CAS  Google Scholar 

  • Boucher, R., Veyrat, R., De Champlain, J., Genest, J.: New procedures for measurement of human plasma angiotensin and renin activity levels. Canad. med. Ass. J. 90, 194 (1964).

    CAS  Google Scholar 

  • Braun-Menedez, E., Fasciolo, J. C., Leloir, L. F., Muñoz, J. M.: The substance causing renal hypertension. J. Physiol. 98, 283 (1940).

    Google Scholar 

  • Dekanski, J.: Quantitative assay of vasopressin. Brit. J. Pharmacol. 7, 567 (1952).

    PubMed  CAS  Google Scholar 

  • Dekanski, J.: The quantitative assay of angiotensin. Brit. J. Pharmacol. 9, 187 (1954).

    PubMed  CAS  Google Scholar 

  • Dexter, L., Haynes, F. W., Bridges, W. C.: Renal humoral pressor mechanism in man; preparation and assay of human renin, human hypertensinogen and hypertensin. J. clin. Invest. 24, 62 (1945).

    Article  PubMed  CAS  Google Scholar 

  • Finkelman, B.: On nature of inhibition in intestines. J. Physiol. 70, 145 (1930).

    Google Scholar 

  • Gaddum, J. H.: The technique of superfusion. Brit. J. Pharmacol. 8, 321 (1953).

    PubMed  CAS  Google Scholar 

  • Hodge, R. L., Lowe, R. D., Vane, J. R.: The effects of alteration of blood volume in the concentration of circulating angiotensin in anaesthetized dogs. J. Physiol. 185, 613 (1966).

    PubMed  CAS  Google Scholar 

  • Hodge, R. L., Ng, K. K. G., Vane, J. R.: Disappearance of angiotensin from the circulation of the dog. Nature (Lond.) 215, 138 (1967).

    Article  CAS  Google Scholar 

  • Holton, P.: A modification of the method of Dale and Laidlau for standardization of posterior pituitary extracts. Brit. J. Pharmacol. 3, 328 (1948).

    PubMed  CAS  Google Scholar 

  • Houssay, B. A, Taquini, A. C.: Action vaso-constrictrice du sang veineux du renin ischémié. C. R. Soc. Biol. (Paris) 128, 1125 (1938).

    CAS  Google Scholar 

  • Ing, T. S., Anderson, R. N., Passovoy, M., Lucís, O. J.: Dose-response relationship in angiotensin bioassay. Cardiovasc. Res. 2, 39 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Khairallah, P. A., Page, I. H.: A vasopressor lipid in incubated plasma. Amer. J. Physiol. 199, 341 (1960).

    PubMed  CAS  Google Scholar 

  • Kwiatkowski, H.: Observations on relation of histamine to reactive hyperaemia. J. Physiol. 100, 147 (1941).

    PubMed  CAS  Google Scholar 

  • Ludueña, F. P.: Acción de los preparados de hipertensina sobre los musculos lisas. Rev. Soc. argent. Biol. 16, 1358 (1940).

    Google Scholar 

  • Masson, G. M. C., Helmcke, G., Corcoran, A. C., Page, I. H.: Pressor and myotrophic activities of incubated plasma. Proc. Soc. exp. Biol. (N. Y.) 94, 544 (1957).

    CAS  Google Scholar 

  • Mccubbin, J. W., Page, I. H.: Renal inhibition of pressor responses tojdrugs. Circulat. Res. 2, 35 (1954).

    PubMed  CAS  Google Scholar 

  • Page, I. H.: Method for perfusion of rabbit ears, and its application to study of renin-angio- tonin vasopressor system, with a note on angiotonin tachyphylaxis. Amer. Heart J. 23, 336 (1942).

    Article  Google Scholar 

  • Page, I. H., Helmer, O. M.: (1) A crystalline pressor substance (angiotonin) resulting from the reaction between renin and renin activator. J. exp. Med. 71, 29 (1940).

    Article  PubMed  CAS  Google Scholar 

  • Page, I. H., Helmer, O. M.: (2) Angiotonin-activator, renin- and angiotensin-inhibitor, and the mechanism of angiotonin tachyphylaxis in normal hypertensive and nephrectomized animals. J. exp. Med. 71, 495 (1940).

    Article  PubMed  CAS  Google Scholar 

  • Page, I. H., Mccubbin, J. W.: Buffer reflexes, tolerance to ganglioplegics and their relationship to enhanced pressor responsiveness. Amer. J. Physiol. 197, 217 (1959).

    PubMed  CAS  Google Scholar 

  • Page, I. H., Mccubbin, J. W.: Mechanisms by which ganglioplegics and atropine enhance cardiovascular responsiveness. Amer. J. Physioi. 205, 1 (1963).

    CAS  Google Scholar 

  • Peach, M. J.: Adrenal medullary stimulation induced by angiotensin I, angiotensin II and analogues. Circulat. Res. 28 (Suppi. II), 11–107 (1971).

    Google Scholar 

  • Peach, M. J., Bumpus, F. M., Khairallah, P. A.: Release of adrenal catecholamines by angiotensin I. J. Pharmacol, exp. Ther. 176, 366 (1971).

    CAS  Google Scholar 

  • Peart, W. S.: A new method of large scale preparation of hypertensin, with a note on its assay. Biochem. J. 59, 300 (1955).

    PubMed  CAS  Google Scholar 

  • Pickens, P. T., Bumpus, F. M., Lloyd, A. M., Smeby, R. R., Page, I. H.: Measurement of renin activity in human plasma. Circulat. Res. 17, 438 (1965).

    PubMed  CAS  Google Scholar 

  • Regoli, D., Vane, J. R.: A sensitive method for the assay of angiotensin. Brit. J. Pharmacol. 23, 351 (1964).

    PubMed  CAS  Google Scholar 

  • Robinson, R. L., Watts, D. T.: An automated trihydroxy indole procedure for the differential analysis of catecholamines. Clin. Chem. 11, 986 (1965).

    PubMed  CAS  Google Scholar 

  • Rudinger, J., Krejcí, I.: Dose-response relations for some synthetic analogues of oxytocin, and the mode of action of oxytocin on the isolated uterus. Experientia (Basel) 18, 585 (1962).

    Article  CAS  Google Scholar 

  • Schwarz, H., Masson, G. M. C., Page, I. H.: A method of assay of the oxytocic activity of angiotonin preparations on the rat’s uterus. J. Pharmacol, exp. Therap. 114, 418 (1955).

    CAS  Google Scholar 

  • Türker, R. K., Yamamoto, M., Bumpus, F. M., Khairallah, P. A.: (1) Loss of activity of angiotensins I and II by superfusion over rabbit aorta and rat colon. Pharmacologist 13, 214 (1971).

    Google Scholar 

  • Türker, R. K., Yamamoto, M., Khairallah, P. A., Bumpus, F. M.: (2) Competitive antagonism of 8-ala-angiotensin II to angiotensin II on isolated rabbit aorta and rat ascending colon. Europ. J. Pharmacol. 15, 285 (1971).

    Article  Google Scholar 

  • Vane, J. R.: A sensitive method for the assay of 5-hydroxy tryptamine. Brit. J. Pharmacol. 12, 344 (1957).

    PubMed  CAS  Google Scholar 

  • Vane, J. R.: The use of isolated organs for detecting active substances in the circulating blood. Brit. J. Pharmacol. 23, 360 (1964).

    PubMed  CAS  Google Scholar 

  • Vane, J. R.: The release and fate of vaso-active hormones in the circulation. Brit. J. Pharmacol. 35, 209 (1969).

    CAS  Google Scholar 

  • Yamamoto, M., Türker, R. K., Bumpus, F. M., Khairallah, P. A.: Loss of activity of angiotensins I and II in isolated superfused rat aorta and in perfused heart and kidney. Amer. J. Physiol. (1972) (in press).

    Google Scholar 

Download references

Authors
  1. Philip A. Khairallah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert R. Smeby
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

    Irvine H. Page M.D. (Emeritus Director of Research) (Emeritus Director of Research)

  2. Division of Research, The Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

    F. Merlin Bumpus Ph.D. (Chairman) (Chairman)

Rights and permissions

Reprints and permissions

Copyright information

© 1974 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Khairallah, P.A., Smeby, R.R. (1974). Bioassay of Angiotensin. In: Page, I.H., Bumpus, F.M. (eds) Angiotensin. Handbuch der experimentellen Pharmakologie / Handbook of Experimental Pharmacology, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65600-2_12

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-65600-2_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65602-6

  • Online ISBN: 978-3-642-65600-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation