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Mechanism of Protein Synthesis and Use of Inhibitors in the Study of Protein Synthesis

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Progress in Molecular and Subcellular Biology 3

Part of the book series: Progress in Molecular and Subcellular Biology ((PMSB,volume 3))

Abstract

The biosynthesis of proteins appears to be one of the most complicated synthetic processes catalyzed by biological systems. It is unique in that as many as three to four energy-rich bonds are consumed in the synthesis of a relatively low-energy chemical bond, the peptide linkage. At least nine non-ribosomal soluble protein factors participate in an orderly sequential series of polymerization reactions of aminoacyl tRNA catalyzed by a most complicated biological synthetic machinery — the ribosomes. Protein synthesis can be divided into four major steps, namely, aminoacylation of tRNA, initiation of the polypeptide chain, polypeptide chain elongation, and termination of chain elongation including release of completed chains from the ribosomes. The first step, aminoacylation of tRNA, will not be discussed in this article.

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References

  1. Algranati, I. D., Gonzalez, N. S., Bade, E. G.: Physiological role of 70 S ribosomes in bacteria. Proc. nat. Acad. Sci. (Wash.) 62, 574 (1969).

    Article  CAS  Google Scholar 

  2. Anand, N., Davis, B. D.: Effect of streptomycin on Escherichia coli. Nature (Lond.) 185, 22 (1960).

    Article  CAS  Google Scholar 

  3. Anderson, B., Hodgkin, D. C., Viswamitra, M. A.: The structure of thiostrepton. Nature (Lond.) 225, 233 (1970).

    Article  CAS  Google Scholar 

  4. Anderson, J. S., Bretscher, M. S., Clark, B. F. S., Marcker, K. A.: A GTP requirement for binding initiator tRNA to ribosomes. Nature (Lond.) 215, 490 (1967).

    Article  CAS  Google Scholar 

  5. Allen, D. W., Zamecnik, P. C.: The effect of puromycin on rabbit reticulocyte ribosomes. Biochim. biophys. Acta (Amst.) 55, 865 (1962).

    Article  CAS  Google Scholar 

  6. Apirion, D.: Three genes that affect Escherichia coli ribosomes. J. molec. Biol. 30, 255 (1967).

    PubMed  CAS  Google Scholar 

  7. Apirion, D., Schlessinger, D., Phillips, S., Sypherd, P.: Escherichia coli: reversion from streptomycin dependence, a mutation in a specific 30 S ribosomal protein. J. molec. Biol. 43, 327 (1969).

    Article  PubMed  CAS  Google Scholar 

  8. Arlinghaus, R. J., Shaeffer, J., Schweet, R.: Mechanism of peptide bond formation in polypeptide synthesis. Proc. nat. Acad. Sci. (Wash.) 51, 1291 (1964).

    Article  CAS  Google Scholar 

  9. Baliga, B. S., Cohen, S. A., Munro, H. N.: Effect of cycloheximide on the reaction of puromacin with polysome-bound peptidyl tRNA. FEBS Letters 8, 249 (1970).

    Article  PubMed  CAS  Google Scholar 

  10. Berissi, H., Groner, Y., Revel, M.: Effect of a purified initiation factor F3 (B) on the selection of ribosomal binding sites on phage MS2 RNA. Nature (Lond.) New Biol. 234, 44 (1971).

    CAS  Google Scholar 

  11. Berman, M. L., Monier, R.: Influence of the 30 S ribosomal subunit on the peptidyl transferase activity of the 50 S ribosomal subunit from E. coli. Biochimie 53, 233 (1971).

    Article  PubMed  CAS  Google Scholar 

  12. Bhuyan, B. K.: Pactamycin, an antibiotic that inhibits protein synthesis. Biochem. Pharmacol. 16, 1411 (1967).

    Article  PubMed  CAS  Google Scholar 

  13. Birge, E. A., Kurland, C. G.: Altered ribosomal protein in streptomycin dependent Escherichia coli. Science 166, 1282 (1969).

    Article  PubMed  CAS  Google Scholar 

  14. Bissel, D. M.: Formation of an altered enzyme by Escherichia coli in the presence of neomycin. J. molec. Biol. 14, 619 (1965).

    Article  Google Scholar 

  15. Bodley, J. W., Davie, E. W.: A study of the mechanism of ambiguous amino acid coding by poly U: the nature of the products. J. molec. Biol. 18, 344 (1966).

    Article  PubMed  CAS  Google Scholar 

  16. Bodley, J. W., Lin, L.: Interaction of E. coli and G-factor with the 50 S ribosomal subunit. Nature (Lond.) 227, 60 (1970).

    Article  CAS  Google Scholar 

  17. Bodley, J. W., Zieve, F. J., Lin, L., Zieve, S. T.: Formation of the ribosome-G-factor-GDP complex in the presence of fusidic acid. Biochem. biophys. Res. Commun. 37, 437 (1969).

    Article  PubMed  CAS  Google Scholar 

  18. Bodley, J. W., Zieve, F. J., Lin, L., Zieve, S. T.: Studies on translocation III. Conditions necessary for the formation and detection of a stable ribosome-G-factor-GDP complex in the presence of fusidic acid. J. biol. Chem. 245, 5656 (1970).

    PubMed  CAS  Google Scholar 

  19. Bodley, J. W., Zieve, F. J., Lin, L.: Studies on translocation IV. The hydrolysis of a single round of GTP in the presence of fusidic acid. J. biol. Chem. 245, 5662 (1970).

    PubMed  CAS  Google Scholar 

  20. Bodley, J. W., Lin, L., Highland, J. H.: Studies on translocation VI. Thiostrepton prevents the formation of a ribosome-G-factor-guanosine triphosphate complex. Biochem. biophys. Res. Commun. 41, 1406 (1970).

    Article  PubMed  CAS  Google Scholar 

  21. Boedtker, H.: The reaction of ribonucleic acid with formaldehyde. I. Optical absorbance studies Biochemistry 6, 2718 (1967).

    Article  PubMed  CAS  Google Scholar 

  22. Breckenridge, L., Gorini, L.: The dominance of streptomycin sensitivity re-examined. Proc. nat. Acad. Sci. (Wash.) 62, 979 (1969).

    Article  CAS  Google Scholar 

  23. Brenner, S., Beckwith, J. R.: Ochre mutants, a new class of suppressible nonsense mutants. J. molec. Biol. 13, 629 (1965).

    Article  Google Scholar 

  24. Brenner, S., Barnett, L., Katz, E. R., Crick, F. H. C.: UGA: a third nonsense triplet in the genetic code. Nature (Lond.) 213, 449 (1967).

    Article  CAS  Google Scholar 

  25. Bretscher, M. S.: Translocation in protein synthesis: a hybrid structure model. Nature (Lond.) 218, 675 (1968).

    Article  CAS  Google Scholar 

  26. Bretscher, M. S., Marcker, K. A.: Polypeptidyl-s RNA and aminoacyl-s RNA binding sites on ribosomes. Nature (Lond.) 211, 380 (1966).

    Article  CAS  Google Scholar 

  27. Brot, N., Redfield, B., Weissbach, H.: Studies on the reaction of the aminoacyl-tRNA-Tu-GTP complex with ribosomal subunits. Biochem. biophys. Res. Commun. 41, 1388 (1970).

    Article  PubMed  CAS  Google Scholar 

  28. Brot, N., Spears, C. L., Weissbach, H.: The formation of a complex containing ribosomes, transfer factor G and a guanosine nucleotide. Biochem. biophys. Res. Commun. 34, 843 (1969).

    Article  PubMed  CAS  Google Scholar 

  29. Brot, N., Spears, C. L., Weissbach, H.: The interaction of transfer factor G, ribosomes and guanosine nucleotides in the presence of fusidic acid. Arch. Biochem. Biophys. 143, 286 (1971).

    Article  PubMed  CAS  Google Scholar 

  30. Brown, J. C., Smith, A. E.: Initiator codons in eukaryotes. Nature (Lond.) 226, 610 (1970).

    Article  CAS  Google Scholar 

  31. Brownstein, B. L., Lewandowski, L. J.: A mutation supressing streptomycin dependence. I. An effect on ribosome formation. J. molec. Biol. 25, 99 (1967).

    Article  PubMed  CAS  Google Scholar 

  32. Busiello, E., Digirolamo, M., Felicetti, L.: Role of mammalian ribosomal subunits and elongation factors in poly U-directed protein synthesis. Biochim. biophys. Acta (Amst.) 228, 289 (1971).

    CAS  Google Scholar 

  33. Cameron, H. J., Julian, G. R.: The effect of chloramphenicol on the polysome formation of starved stringent Escherichia coli. Biochim. biophys. Acta. (Amst.) 169, 373 (1968).

    CAS  Google Scholar 

  34. Capecchi, M. R.: Polypeptidyl chain termination in vitro. Isolation of a release factor. Proc. nat. Acad. Sci. (Wash.) 58, 1144 (1967).

    Article  CAS  Google Scholar 

  35. Capecchi, M. R., Klein, H. A.: Characterization of three proteins involved in polypeptide chain termination. Cold Spr. Harb. Symp. quant. Biol. 34, 469 (1969).

    CAS  Google Scholar 

  36. Cardani, C. D., Ghiringhelli, R., Mondelli, R., Pavan, M., Quilico, A.: Properties biologiques et composition chimique de la pederine. Ann. Soc. Entomol. Franc. 1, 813 (1965).

    CAS  Google Scholar 

  37. Casjens, S. R., Morris, A. J.: The selective inhibition of protein assembly by gougerotin. Biochim. biophys. Acta (Amst.) 108, 677 (1965).

    CAS  Google Scholar 

  38. Cerna, J., Jonak, J., Rychlik, I.: Effects of macrolide antibiotics on the ribosomal peptidyl transferase in cell-free systems derived from E. coli B and erythromycin-resistant mutant of E. coli B. Biochim. biophys. Acta (Amst.) 240, 109 (1971).

    CAS  Google Scholar 

  39. Cerna, J., Lichtenhaler, F. W., Rychlik, I.: The effect of gougerotin analogues on ribosomal peptidyl transferase. FEBS Letters 14, 45 (1971).

    Article  PubMed  CAS  Google Scholar 

  40. Cerna, J., Rychlik, I., Pulkrabek, P.: The effect of antibiotics on the coded binding of peptidyl tRNA to the ribosome and on the transfer o5 the peptidyl residue to puromycin. Europ. J. Biochem. 9, 27 (1969).

    Article  PubMed  CAS  Google Scholar 

  41. Cerna, J., Rychlik, I., Zemlicka, J., Chladek, S.: Substrate specificity of ribosomal peptidyl transferase II 2’(3’)-O-aminoacyl nucleosides as acceptors of the peptide chain in the fragment reaction. Biochim. biophys. Acta (Amst.) 204, 203 (1970).

    CAS  Google Scholar 

  42. Chae, Y-B., Mazumder, R., Ochoa, S.: Polypeptide chain initiation in E. coli. Studies on the function of initiation factor F1. Proc. nat. Acad. Sci. (Wash.) 63, 828 (1969).

    Article  CAS  Google Scholar 

  43. Chang, F. N., Flaks, J. G.: Topography of the E. coli 30 S ribosomal subunk and streptomycin binding. Proc. nat. Acad. Sci. (Wash.) 67, 1321 (1970).

    Article  CAS  Google Scholar 

  44. Chang, F. N., Weisblum, B.: The specific of lincomycin binding to ribosomes. Biochemistry 6, 836 (1967).

    Article  PubMed  CAS  Google Scholar 

  45. Chen, Chong-Maw, Ofengand, J.: Inactivation of the Tu-GTP recognition site in aminoacyl-tRNA by chemical modification of the tRNA. Biochem. biophys. Res. Commun. 41, 190 (1970).

    Article  PubMed  CAS  Google Scholar 

  46. Chuang, D. M., Simpson, M. V.: A translocation-associated ribosomal conformational change detected by hydrogen exchange and sedimentation velocity. Proc. nat. Acad. Sci. (Wash.) 68, 1474 (1971).

    Article  CAS  Google Scholar 

  47. Clark, B. F. C., Marcker, K. A.: The role of N-formylmethionyl sRNA in protein biosynthesis. J. molec. Biol. 17, 394 (1966).

    Article  PubMed  CAS  Google Scholar 

  48. Clark, J. M., Chang, A. Y.: Inhibitors of the transfer of amino acids from aminoacyl soluble ribonucleic acid to proteins. J. biol. Chem. 240, 4734 (1965).

    PubMed  CAS  Google Scholar 

  49. Cocito, C.: Metabolism of macromolecules in bacteria treated with virginiamycin. J. gen. Microbiol. 57, 179 (1969).

    PubMed  CAS  Google Scholar 

  50. Cocito, C., Kaji, A.: Virginiamycin M: a specific inhibitor of the acceptor site of ribosomes. Biochimie 53, 763 (1971).

    Article  PubMed  CAS  Google Scholar 

  51. Cohen, B. B.: Two fractions required for cell-free protein synthesis with components from rabbit reticulocytes. Biochem. J. 115, 523 (1969).

    PubMed  CAS  Google Scholar 

  52. Cohen, L. B., Goldberg, I. H.: Inhibition of peptidyl-sRNA binding to ribosomes by pactamycin. Biochem. biophys. Res. Commun. 29, 617 (1967).

    Article  PubMed  CAS  Google Scholar 

  53. Cohen, L. B., Goldberg, I., Herner, A.: Inhibition by pactamycin of the initiation of protein synthesis. Effect on the 30 S ribosomal subunit. Biochemistry 8, 1327 (1969).

    Article  PubMed  CAS  Google Scholar 

  54. Colombo, B., Felicetti, L., Baglioni, C.: Inhibition of protein synthesis in reticulocytes by antibiotics — Effects on polysomes. Biochim. biophys. Acta (Amst.) 119, 109 (1966).

    CAS  Google Scholar 

  55. Connamacher, R. H., Mandel, H. G.: Studies on the intracellular localization of tetracycline in bacteria. Biochim. biophys. Acta (Amst.) 166, 475 (1968).

    CAS  Google Scholar 

  56. Cooper, D., Banthorpe, D., Wilkie, D.: Modified ribosomes conferring resistance to cycloheximide in mutants of saccharomyces cerevisiae. J. molec. Biol. 26, 347 (1967).

    Article  PubMed  CAS  Google Scholar 

  57. Cooper, D., Gordon, J.: Effect of aminoacyl transfer RNA on competition between guanosine 5’-triphospbate and guanosine 5′-diphosphate for binding to a polypeptide chain elongation factor from Escherichia coli. Biochemistry 8, 4289 (1969).

    Article  PubMed  CAS  Google Scholar 

  58. Culp, W. J., McKeehan, W. L., Hardesty, B.: Deacylated tRNAphe binding to a reticulocyte ribosomal site for the initiation of polyphenylalanine synthesis. Proc. nat. Acad. Sci. (Wash.) 63, 1431 (1969).

    Article  CAS  Google Scholar 

  59. Culp, W. J., Morrisey, J., Hardesty, B.: Initiator tRNA for the synthesis of globin peptides. Biochem. biophys. Res. Commun. 40, 777 (1970).

    Article  PubMed  CAS  Google Scholar 

  60. Cundliffe, E.: Antibiotics and polyribosomes. II. Some effects of lincomycin, spiramycin and streptomycin A in vivo. Biochemistry 8, 2063 (1969).

    Article  PubMed  CAS  Google Scholar 

  61. Cundliffe, E.: The mode of action of thiostrepton in vivo, Biochem. biophys. Res. Commun. 44, 912 (1971).

    Article  PubMed  CAS  Google Scholar 

  62. Cundliffe, E.: Polyribosomes and ribosomal subunits of bacterial protoplasts. Biochem. biophys. Res. Commun. 33, 247 (1968).

    Article  PubMed  CAS  Google Scholar 

  63. Cundliffe, E., McQuillen, K.: Bacterial protein synthesis: the effects of antibiotics. J. molec. Biol. 30, 137 (1967).

    Article  PubMed  CAS  Google Scholar 

  64. Das, H. K., Goldstein, A., Kanner, L. C.: Inhibition by chloramphenicol of the growth of nascent protein chains in Escherichia coli. Molec. Pharmacol. 2, 158 (1966).

    CAS  Google Scholar 

  65. Davies, J., Gilbert, W., Gorini, L.: Streptomycin, suppression and the code. Proc. nat. Acad. Sci. (Wash.) 51, 883 (1964).

    Article  CAS  Google Scholar 

  66. Davies, J., Jones, D. S., Khorana, H. G.: A further study of misreading of codons induced by streptomycin and neomycin using ribonucleotides in alternating sequence as templates. J. molec. Biol. 18, 48 (1966).

    Article  PubMed  CAS  Google Scholar 

  67. Davies, B. D.: Action of aminoglycoside antibiotics. Asian med. J. 11, 78 (1968).

    Google Scholar 

  68. Day, L. E.: Tetracycline inhibition of cell-free protein synthesis I. Binding of tetracycline to components of the system. J. Bact. 91, 1917 (1966).

    PubMed  CAS  Google Scholar 

  69. Dintzis, H. M. L.: Assembly of the peptide chains of hemoglobin. Proc. nat. Acad. Sci. (Wash.) 47, 247 (1961).

    Article  CAS  Google Scholar 

  70. Dresden, M. H., Hoagland, M. B.: Polyribosomes of Escherichia coli. Breakdown during glucose starvation. J. biol. Chem. 242, 1065 (1967).

    PubMed  CAS  Google Scholar 

  71. Dube, S. K., Rudland, P. S.: Control of translation by T4 phage: Altered binding of disfavoured messenger. Nature (Lond.) 226, 820 (1970).

    Article  CAS  Google Scholar 

  72. Dubnoff, J. S., Maitra, U.: Protein factors involved in polypeptide chain initiation in E. coli. Cold Spr. Harb. Symp. quant. Biol. 34, 301 (1969).

    CAS  Google Scholar 

  73. Ellis, R. J., Hartley, M. R.: Sites of synthesis of chloroplast proteins. Nature (Lond.) New Biol. 233, 193 (1971).

    Article  CAS  Google Scholar 

  74. Ennis, H. L.: Synergistin: a synergistic antibiotic complex which selectively inhibits protein synthesis. Proc. Int. Cong. Chemotherapy, 6th, Tokyo 2, 489 (1970).

    CAS  Google Scholar 

  75. Ennis, H. L.: Interaction of vernamycin A with Escherichia coli ribosomes. Biochemistry 10, 1265 (1971).

    Article  PubMed  CAS  Google Scholar 

  76. Erbe, R. W., Nau, M. M., Leder, P.: Translation and translocation of defined RNA messengers. J. molec. Biol. 39, 441 (1969).

    Article  PubMed  CAS  Google Scholar 

  77. Fahnstock, S., Neumann, H., Shashoua, V., Rich, A.: Ribosome-catalyzed ester formation. Biochemistry 9, 2477 (1970).

    Article  Google Scholar 

  78. Fernandez-Munoz, R., Monro, R. E., Torres Pinedo, R., Vazquez, D.: Substrates and antibiotic binding sites at the peptidyl transferase centre of Escherichia coli ribosomes. Studies on the chloramphenicol, lincomycin and erythromycin sites. Europ. J. Biochem. 23, 185 (1971).

    Article  PubMed  CAS  Google Scholar 

  79. Flessel, C. P.: Chloramphenicol protects polyribosomes. Biochem. biophys. Res. Commun. 32, 438 (1968).

    Article  PubMed  CAS  Google Scholar 

  80. Galasinski, W., Moldave, K.: Purification of aminoacyl transferase II (translocation factor) from rat liver. J. biol. Chem. 244, 6527 (1969).

    PubMed  CAS  Google Scholar 

  81. Ghosh, H. P., Khorana, H. G.: Studies on polynucleotides LXXXIV. On the role of ribosomal subunits in protein synthesis. Proc. nat. Acad. Sci. (Wash.) 58, 2455 (1967).

    Article  CAS  Google Scholar 

  82. Gilbert, W.: Polypeptide synthesis in Escherichia coli. II. The polypeptide chain and sRNA. J. molec. Biol. 6, 389 (1963).

    Article  PubMed  CAS  Google Scholar 

  83. Gill, D. M., Pappenheimer, A. M., Jr., Baseman, J. B.: Studies on transferase LI using diphtheria toxin. Cold Spr. Harb. Symp. quant. Biol. 34, 595 (1969).

    CAS  Google Scholar 

  84. Gill, D. M., Pappenheimer, A. M., Jr., Brown, R., Kurnick, J. T.: Studies on the mode of action of diphtheria toxin. VII. Toxin-stimulated hydrolysis of nicotinomide adenine dinucleotide in mammalian cell extracts. J. exp. Med. 129, 1 (1969).

    Article  PubMed  CAS  Google Scholar 

  85. Godchaux, W. III., Adamson, S. D., Herbert, E.: Effect of cycloheximide on polyribosome function in reticulocytes. J. molec. Biol. 27, 57 (1967).

    Article  PubMed  CAS  Google Scholar 

  86. Goldberg, I. H., Mitsugi, K.: Inhibition by sparsomycin and other antibiotics of the puromycin induced release of polypeptide from ribosomes. Biochemistry 6, 383 (1967).

    Article  PubMed  CAS  Google Scholar 

  87. Goldberg, I. H., Mitsugi, K.: Sparsomycin inhibition of polypeptide synthesis promoted by synthetic and natural polynucleotides. Biochemistry 6, 372 (1967).

    Article  PubMed  CAS  Google Scholar 

  88. Goldstein, J. L., Beaudet, A. L., Caskey, C. T.: Peptide chain termination with mammalian release factor. Proc. nat. Acad. Sci. (Wash.) 67, 99 (1970).

    Article  CAS  Google Scholar 

  89. Goldstein, J. L., Caskey, C. T.: Peptide chain termination: effect of proteins on ribosomal binding of release factors. Proc. nat. Acad. Sci. (Wash.) 67, 537 (1970).

    Article  CAS  Google Scholar 

  90. Goldstein, J. L., Milman, G., Scolnick, E., Caskey, T.: Peptide chain termination, VI. Purification and site of action of s. Proc. nat. Acad. Sci. (Wash.) 65, 430 (1970).

    Article  CAS  Google Scholar 

  91. Goor, R. S., Maxwell, E. S.: The diphtheria toxin-dependent adenosine diphosphate ribosylation of rat liver aminoacyl transferase II. J. biol. Chem. 245, 616 (1970).

    PubMed  CAS  Google Scholar 

  92. Gordon, J.: Hydrolysis of guanosine 5’-triphosphate associated with binding of aminoacyl transfer ribonucleic acid to ribosomes. J. biol. Chem. 244, 5680 (1969).

    PubMed  CAS  Google Scholar 

  93. Gordon, J.: Regulation of the in vivo synthesis of the polypeptide chain. Biochemistry 9, 912 (1970).

    Article  PubMed  CAS  Google Scholar 

  94. Gorini, L.: In formational suppression. Ann. Rev. Genet. 4, 107 (1970).

    Article  PubMed  CAS  Google Scholar 

  95. Gorini, L., Kataja, E.: Phenotypic repair by streptomycin of defective genotypes in E. coli. Proc. nat. Acad. Sci. (Wash.) 51, 487 (1964).

    Article  CAS  Google Scholar 

  96. Gottesman, M. E.: Reactions of ribosome-bound peptidyl transfer ribonucleic acid with aminoacyl transfer ribonucleic acid or puromycin. J. biol. Chem. 242, 5564 (1967).

    PubMed  CAS  Google Scholar 

  97. Greenshpan, H., Revel, M.: Initiator protein dependent binding of messenger RNA to ribosomes. Nature (Lond.) 224, 331 (1969).

    Article  CAS  Google Scholar 

  98. Grollman, A. P., Stewart, M. L.: Inhibition of the attachment of messenger ribonucleic acid to ribosomes. Proc. nat. Acad. Sci. (Wash.) 61, 719 (1968).

    Article  CAS  Google Scholar 

  99. Groner, Y., Herzberg, M., Revel, M.: Translation initiation factor C(f2): selective inactivation of its f-Met-tRNA binding activity which does not affect messenger RNA binding to the 30 S ribosome. FEBS Letters 6, 315 (1970).

    Article  PubMed  CAS  Google Scholar 

  100. Grunberg-Manago, M., Robinowitz, J. C., Dondon, J., Lelong, J. C., Gros, F.: Different classes of initiation factor F3 and their dissociation activity. FEBS Letters 19, 193 (1971).

    Article  PubMed  CAS  Google Scholar 

  101. Gualerzi, C., Pon, C. L., Kaji, A.: Initiation factor dependent release of aminoacyl-tRNA’s from complexes of 30 S ribosomal subunits, synthetic polynucleotide, and aminoacyl tRNA. Biochem. biophys. Res. Commun. 45, 1312 (1971).

    Article  PubMed  CAS  Google Scholar 

  102. Gurgo, C., Apirion, D., Schlessinger, D.: Effects of chloramphenicol and fusidic acid on polyribosome metabolism in Escherichia coli. FEBS Letters 3, 34 (1969).

    Article  PubMed  CAS  Google Scholar 

  103. Gurgo, C., Apirion, D., Schlessinger, D.: Polyribosome metabolism in Escherichia coli treated with chloramphenicol, neomycin, spectinomycin or tetracycline. J. molec. Biol. 45, 205 (1969).

    Article  PubMed  CAS  Google Scholar 

  104. Guthrie, C., Nomura, M.: Initiation of protein synthesis: a critical test of the 30 S subunit model. Nature (Lond.) 219, 232 (1968).

    Article  CAS  Google Scholar 

  105. Hachmann, J., Miller, D. L., Weissbach, H.: Purification of factor Ts: studies on the formation and stability of nucleotide complexes containing transfer factor Tu. Arch. Biochem. Biophys. 147, 457 (1971).

    Article  PubMed  CAS  Google Scholar 

  106. Hawthorne, D. C.: Identification of nonsense codons in yeast. J. molec. Biol. 43, 71 (1969).

    Article  PubMed  CAS  Google Scholar 

  107. Heintz, R., McAllister, H., Arlinghaus, R., Schweet, R.: Formation and function of the active ribosome complex. Cold Spr. Harb. Symp. quant. Biol. 31, 633 (1966).

    CAS  Google Scholar 

  108. Helser, T. L., Davtes, J. E., Dahlberg, J. E.: Mechanism of kasugamycin resistance in E. coli. Nature (Lond.) New Biol. 235, 6 (1972).

    CAS  Google Scholar 

  109. Hershey, J. W. B., Dewey, K. F., Thach, R. E.: Purification and properties of initiation factor f-1. Nature (Lond.) 222, 944 (1969).

    Article  CAS  Google Scholar 

  110. Herzberg, M., Lelong, J. C., Revel, M.: Purification of initiator C from Escherichia coli: 2l protein which binds messenger RNA and initiator tRNA to the 30 S ribosome. J. molec. Biol. 44, 297 (1969).

    Article  PubMed  CAS  Google Scholar 

  111. Hettinger, T. P., Craig, L. C.: Edeine. IV. Structures of the antibiotic peptides edeines A1 and B1. Biochemistry 9, 1224 (1970).

    Article  PubMed  CAS  Google Scholar 

  112. Hierowski, M., Kurylo-Borowska, Z.: On the mode of action of edeine. I. Effect of edeine on the synthesis of polyphenylalanine in a cell-free system. Biochim. biophys. Acta (Amst.) 95, 578 (1965).

    CAS  Google Scholar 

  113. Hirashima, A., Kaji, A.: Release of ribosomes from messenger RNA—a process dependent on G-factor and an additional factor. J. molec. Biol. (1972) (in press).

    Google Scholar 

  114. Hoeksema, H.: Chemical studies on lincomycin I. The structure of lincomycin. J. Amer. chem. Soc. 86, 4223 (1964).

    Article  CAS  Google Scholar 

  115. Hoerz, W., McCarty, K. S.: Evidence for a proposed initiation complex for protein synthesis in reticulocyte polyribosome profiles. Proc. nat. Acad. Sci. (Wash.) 63 1206 (1969).

    Article  CAS  Google Scholar 

  116. Honjo, T., Nishizuka, Y., Hayaishi, O., Kato, I.: Diphtheria toxin-dependent adenosine diphosphate ribosylation of aminoacyl transferase II and inhibition of protein synthesis. J. biol. Chem. 243, 3553 (1968).

    PubMed  CAS  Google Scholar 

  117. Hosokawa, K., Fujimura, R. K., Nomura, M.: Reconstitution of functionally active ribosomes from inactive subparticles and proteins. Proc. nat. Acad. Sci. (Wash.) 55, 198 (1966).

    Article  CAS  Google Scholar 

  118. Hsu, W-T., Weiss, S. B.: Selective translation of T4 template RNA by ribosomes from T4-infected Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 64, 345 (1969).

    Article  CAS  Google Scholar 

  119. Hurwitz, C., Braun, C. B.: Measurement of binding of chloramphenicol by intact cells. J. Bact. 93, 1671 (1967).

    PubMed  CAS  Google Scholar 

  120. Ibuki, F., Moldave, K.: The effect of guanosine triphosphate, other nucleotides and aminoacyl transfer RNA on the activity of transferase I and on its binding to ribosomes. J. biol. Chem. 243, 44 (1968).

    PubMed  CAS  Google Scholar 

  121. Igarashi, K., Ishitsuka, H., Kaji, A.: Comparative studies on the mechanism of action of lincomycin, streptomycin and erythromycin. Biochem. biophys. Res. Commun. 37, 499 (1969).

    Article  PubMed  CAS  Google Scholar 

  122. Igarashi, K., Kaji, A.: On the nature of two ribosomal sites for specific sRNA binding. Proc. nat. Acad. Sci. (Wash.) 58, 1971 (1967).

    Article  CAS  Google Scholar 

  123. Igarashi, K., Kaji, A.: Evidence for one functional phenylalanyl tRNA binding site on the 30 s ribosomal subunit. Proc. nat. Acad. Sci. (Wash.) 62, 498 (1969).

    Article  CAS  Google Scholar 

  124. Igarashi, K., Kaji, A.: Relationship between sites 1,2 and acceptor, donor sites for the binding of aminoacyl tRNA to ribosomes. Europ. J. Biochem. 14, 41 (1970).

    Article  PubMed  CAS  Google Scholar 

  125. Igarashi, K., Tanaka, S., Kaji, A.: On the aminoacyl-tRNA binding site of the 30 s ribosomal subunit and its relation to the chain initiation site of the ribosome. Biochim. biophys. Acta (Amst.) 228, 728 (1971).

    CAS  Google Scholar 

  126. Infante, A., Baierlein, R.: Pressure-induced dissociation of sedimenting ribosomes: effect on sedimentation patterns. Proc. nat. Acad. Sci. (Wash.) 68, 1780 (1971).

    Article  CAS  Google Scholar 

  127. Irvin, J. D., Julian, G. R.: The distribution of 14C-proline peptides synthesized in vitro directed by polycytidylic acid; the effect of chloramphenicol. FEBS Letters 8, 129 (1970).

    Article  PubMed  CAS  Google Scholar 

  128. Ishitsuka, H., Kaji, A.: Prevention of ribosomal donor site from being occupied by aminoacyl tRNA during polypeptide synthesis. FEBS Letters (1972) (in press).

    Google Scholar 

  129. Ishitsuka, H., Kaji, A.: Release of tRNA by breakdown of messenger RNA. Biochim. biophys. Acta (Amst.) (1972) (in press).

    Google Scholar 

  130. Ishitsuka, H., Kuriki, Y., Kaji, A.: Release of transfer ribonucleic acid from ribosomes. A G-factor and guanosine triphosphate-dependent reaction. J. biol. Cjhem. 245, 3346 (1970).

    CAS  Google Scholar 

  131. Iwasaki, K., Mizumoto, K., Tanaka, M., Kaziro, Y.: Studies on the in vitro system for protein synthesis from mammalian organs. Seikagaku 43, 722 (1971).

    Google Scholar 

  132. Iwasaki, K., Sabol, S. L., Wahba, A. J., Ochoa, S.: Translation of the genetic message VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes. Arch. Biochem. Biophys. 125, 542 (1968).

    Article  PubMed  CAS  Google Scholar 

  133. Jacobs-Lorena, M., Brega, A., Baglioni, C.: Inhibition of protein synthesis in reticulocytes by antibiotics. V. Mechanism of action of pederine, an inhibitor of initiation and elongation. Biochem. biophys. Acta (Amst.) 240, 263 (1971).

    CAS  Google Scholar 

  134. Jayaraman, J., Goldberg, I. H.: Localization of sparsomycin action to the peptide-bond forming step. Biochemistry 7, 418 (1968).

    Article  PubMed  CAS  Google Scholar 

  135. Jerez, C., Sandoval, A., Allende, J. E., Henes, C., Ofengand, J.: Specificity of the interaction of aminoacyl RNA with a protein-guanosine triphosphate complex from wheat embryo. Biochemistry 8, 3006 (1969).

    Article  PubMed  CAS  Google Scholar 

  136. Julian, G. R.: 14C lysine peptides synthesized in an in vitro Escherichia coli system in the presence of chloramphenicol. J. molec. Biol. 12, 9 (1965).

    Article  PubMed  CAS  Google Scholar 

  137. Kaempfer, R.: Control of single ribosome formation by an initiation factor for protein synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 2458 (1971).

    Article  CAS  Google Scholar 

  138. Kaempfer, R., Messelson, M., Raskas, H. J.: Cyclic dissociation into stable subunits and reformation of ribosomes during bacterial growth. J. molec. Biol. 31, 277 (1968).

    Article  PubMed  CAS  Google Scholar 

  139. Kaji, A.: Partial proteolytic digestion of yeast hexokinase and its relation to multiple forms of the enzyme. Arch. Biochem. Biophys. 112, 54 (1965).

    Article  PubMed  CAS  Google Scholar 

  140. Kaji, A., Kaji, H.: Specific interaction of soluble RNA with polyribonucleic acid induced polysomes. Biochem. biophys. Res. Commun. 13, 186 (1963).

    Article  CAS  Google Scholar 

  141. Kaji, H.: Intraribosomal environment of the nascent peptide chain. Int. Rev. Cytol. 29, 169 (1970).

    Article  PubMed  CAS  Google Scholar 

  142. Kaji, H.: Genetic code and streptomycin: their relation to ribosomal subunits. Biochim. biophys. Acta (Amst.) 134, 134 (1967).

    CAS  Google Scholar 

  143. Kaji, H., Kaji, A.: Specific binding of sRNA with the template-ribosome complex. Proc. nat. Acad. Sci. (Wash.) 52, 1541 (1964).

    Article  CAS  Google Scholar 

  144. Kaji, H., Kaji, A.: Specific binding of sRNA to ribosomes: effect of streptomycin. Proc. nat. Acad. Sci. (Wash.) 54, 213 (1965).

    Article  CAS  Google Scholar 

  145. Kaji, H., Suzuka, I., Kaji, A.: Binding of specific ribonucleic acid to ribosomes. Binding of soluble ribonucleic acid to template — 30 s subunits complex. J. biol. Chem. 241, 1251 (1966).

    PubMed  CAS  Google Scholar 

  146. Kaji, H., Tanaka, Y.: Binding of dihydrostreptomycin to ribosomal subunits. J. molec. Biol. 32, 221 (1968).

    Article  PubMed  CAS  Google Scholar 

  147. Kan, Y. W., Golini, F., Thach, R. E.: A new protein synthesis factor from Escherichia coli, Proc. nat. Acad. Sci. (Wash.) 67, 1137 (1970).

    Article  CAS  Google Scholar 

  148. Kaziro, Y., Inoue, N., Kuriki, Y., Mizumoto, K., Tanaka, M., Kawakita, M.: Purification and properties of factor G. Cold Spr. Harb. Symp. quant. Biol. 34, 385 (1969).

    CAS  Google Scholar 

  149. Kelley, W. S., Schaechter, M.: Magnesium ion-dependent dissociation of polysomes and free 70 s ribosomes in Bacillus megaterium. J. molec. Biol. 42, 599 (1969).

    Article  PubMed  CAS  Google Scholar 

  150. Khairallah, E., Pitot, H. C.: 3′, 5′, cyclic AMP and the release of polysome-bound proteins in vitro, Biochem. biophys. Res. Commun. 29, 269 (1967).

    Article  PubMed  CAS  Google Scholar 

  151. Kinoshita, T., Kawano, G., Tanaka, N.: Association of fusidic acid sensitivity with G-factor in a protein-synthesizing system. Biochem. biophys. Res. Commun. 33, 769 (1968).

    Article  PubMed  CAS  Google Scholar 

  152. Kinoshita, T., Tanaka, N.: On the site of action of bottromycin A2. J. Antibiot. 23, 311 (1970).

    PubMed  CAS  Google Scholar 

  153. Kinoshita, T., Liou, Y-F., Tanaka, N.: Inhibition by thiopeptin of ribosomal functions associated with T and G factors. Biochem. biophys. Res. Commun. 44, 859 (1971).

    Article  PubMed  CAS  Google Scholar 

  154. Kirschmann, C., Davis, B. D.: Phenotypic suppression in E. coli by chloramphenicol and other reversible inhibitors of the ribosome. J. Bact. 98, 152 (1969).

    PubMed  CAS  Google Scholar 

  155. Knippenberg, P. H., van, Ravenswaay Claasen, J. C., Grijm-Vos, M., Veldstra, H., Boson, L.: Stimulation and inhibition of polypeptide synthesis by streptomycin in ribosomal systems of Escherichia coli, programmed with various messengers. Biochim. biophys. Acta (Amst.) 95, 461 (1965).

    Google Scholar 

  156. Kohler, R. E., Ron, F. Z., Davis, B. D.: Significance of the free 70 s ribosomes in Escherichia coli extracts. J. molec. Biol. 36, 71 (1968).

    Article  PubMed  CAS  Google Scholar 

  157. Kolakofsky, D., Dewey, K. F., Hershey, J. W. B., Thach, R. E.: Guanosine 5′-triphosphatase activity of initiation factor f2. Proc. nat. Acad. Sci. (Wash.) 61, 1066 (1968).

    Article  CAS  Google Scholar 

  158. Kreider, G., Brownstein, B.: A mutation suppressing streptomycin dependence. II. An altered protein on the 30 s ribosomal subunit. J. molec. Biol. 61, 135 (1971).

    Article  PubMed  CAS  Google Scholar 

  159. Krembel, J., Apirion, D.: Changes in ribosomal proteins associated with mutants in a locus tbat affects Escherichia coli ribosomes. J. molec. Biol. 33, 363 (1968).

    Article  PubMed  CAS  Google Scholar 

  160. Krisko, I., Gordon, J., Lipmann, F.: Studies on the interchangeability of one of the mammalian and bacterial supernatant factors in protein biosynthesis. J. biol. Chem. 244, 6117 (1969).

    PubMed  CAS  Google Scholar 

  161. Kucan, Z., Lipmann, F.: Differences in chloramphenicol sensitivity of cell-free amino acid polymerization systems. J. biol. Chem. 239, 516 (1964).

    PubMed  CAS  Google Scholar 

  162. Kuechler, E.: Role of GTP in the positioning of formylmethionyl tRNAf on the E. coli ribosome. Nature (Lond.) New Biology 234, 216 (1971).

    Article  CAS  Google Scholar 

  163. Kuntzel, H.: Specificity of mitochondrial and cytoplasmic ribosomes from neurospora crassa. FEBS Letters 4, 140 (1969).

    Article  PubMed  Google Scholar 

  164. Kurylo-Borowska, Z., Hierowski, M.: On the mode of action of edeine. II. Studies on the binding of edeine to Escherichia coli ribosomes. Biochim. biophys. Acta (Amst.) 95, 590 (1965).

    CAS  Google Scholar 

  165. Lai, C. J., Weisblum, B.: Altered methylation of ribosomal RNA in an erythromycin-resistant strain of Staphylococcus aureus. Proc. nat. Acad. Sci. (Wash.) 68, 856 (1971).

    Article  CAS  Google Scholar 

  166. Lee-Huang, S., Ochoa, S.: Messenger discriminating species of initiation factor F3. Nature (Lond.) New Biology 234, 236 (1971).

    CAS  Google Scholar 

  167. Leis, J. P., Keller, E. B.: Protein chain initiation by methionyl-tRNA. Biochem. biophys. Res. Commun. 40, 416 (1970).

    Article  PubMed  CAS  Google Scholar 

  168. Lelong, J. C., Cousin, M. A., Gros, D., Grunberg-Manago, M., Gros, F.: Streptomycin induced release of fmet-tRNA from the ribosomal initiation complex. Biochem. biophys. Res. Commun. 42, 530 (1971).

    Article  PubMed  CAS  Google Scholar 

  169. Lelong, J. C., Grunberg-Manago, M., Dondon, J., Gros, D., Gros, F.: Interaction between guanosine derivatives and factors involved in the initiation of protein synthesis. Nature (Lond.) 226, 505 (1970).

    Article  CAS  Google Scholar 

  170. Lengyel, P., Soll, D.: Mechanism of protein biosynthesis. Bact. Rev. 33, 264 (1969).

    PubMed  CAS  Google Scholar 

  171. Lennette, E., Apirion, D.: The level of fmet-tRNA on ribosomes from streptomycin treated cells. Biochem. biophys. Res. Commun. 41, 804 (1970).

    Article  PubMed  CAS  Google Scholar 

  172. Levinthal, C., Fan, D. P., Higa, A., Ztmmermann, R. A.: The decay and protection of messenger RNA in bacteria. Cold Spr. Harb. Symp. quant. Biol. 28, 183 (1963).

    CAS  Google Scholar 

  173. Lewandowski, L. J., Brownstein, B. L.: An altered pattern of libosome synthesis in a mutant of E. coli. Biochem. biophys. Res. Commun. 25, 554 (1966).

    Article  CAS  Google Scholar 

  174. Lin, Y. C., Kinoshita, T., Tanaka, N.: Mechanism of protein synthesis inhibition by bottromycin A2: Studies with puromycin. J. Antibiot. Ser. A (Tokyo) 21, 471 (1968).

    CAS  Google Scholar 

  175. Lockwood, A. H., Chakraborty, P. R., Maitra, U.: A complex between initiation factor IF2, guanosine triphosphate, and fmet-tRNAf: an intermediate in initiation complex formation. Proc. nat. Acad. Sci. (Wash.) 68, 3122 (1971).

    Article  CAS  Google Scholar 

  176. Lockwood, A. H., Maitra, U.: Function of GTP in polypeptide chain initiation in E. coli. Fed. Proc. (1972) (in press).

    Google Scholar 

  177. Lodish, H. F.: Bacteriophage f2 RNA: control of translation and gene order. Nature (Lond.) 220, 345 (1968).

    Article  CAS  Google Scholar 

  178. Lodish, H. F.: Specificity in bacterial protein synthesis: role of initiation factors and ribosomal subunits. Nature (Lond.) 226, 705 (1970).

    Article  CAS  Google Scholar 

  179. Lodish, H. F., Robertson, H. D.: Regulation of in vitro translation of bacteriophage f2 RNA. Cold Spr. Harb. Symp. quant. Biol. 34, 655 (1969).

    CAS  Google Scholar 

  180. Lucas-Lenard, J., Haenni, A-L.: Release of transfer RNA during peptide chain elongation. Proc. nat. Acad. Sci. (Wash.) 63, 93 (1969).

    Article  CAS  Google Scholar 

  181. Lucas-Lenard, J., Lipmann, F.: Protein biosynthesis. Ann. Rev. Biochem. 40, 409 (1971).

    Article  PubMed  CAS  Google Scholar 

  182. Lucas-Lenard, J., Lipmann, F.: Initiation of polyphenylalanine synthesis by N-acetyl-phenylalanyl sRNA. Proc. nat. Acad. Sci. (Wash.) 57, 1050 (1967).

    Article  CAS  Google Scholar 

  183. Lucas-Lenard, J., Lipmann, F.: Separation of three microbial amino acid polymerization factors. Proc. nat. Acad. Sci. (Wash.) 55, 1562 (1966).

    Article  CAS  Google Scholar 

  184. Lucas-Lenard, J., Tao, P., Haenni, A.-L.: Further studies on bacterial polypeptide elongation. Cold Spr. Harb. Symp. quant. Biol. 34, 455 (1969).

    CAS  Google Scholar 

  185. Luzzatto, L., Apirion, D., Schlessinger, D.: Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis. Proc. nat. Acad. Sci. (Wash.) 60, 873 (1968).

    Article  CAS  Google Scholar 

  186. Luzzatto, L., Apirion, D., Schlessinger, D.: Polyribosome depletion and blockage of the ribosome cycle by streptomycin in Escherichia coli. J. molec. Biol. 42, 315 (1969).

    Article  PubMed  CAS  Google Scholar 

  187. MacDonald, J. S., Goldberg, I. H.: An effect of pactamycin on the initiation of protein synthesis in reticulocytes. Biochem. biophys. Res. Commun. 41, 1 (1970).

    Article  PubMed  CAS  Google Scholar 

  188. McKeehan, W., Hardesty, B.: The mechanism of cycloheximide inhibition of protein synthesis in rabbit reticulocytes. Biochem. biophys. Res. Commun. 36, 625 (1969).

    Article  PubMed  CAS  Google Scholar 

  189. McKeehan, W., Hardesty, B.: Purification and partial characterization of the aminoacyl transfer ribonucleic acid binding enzyme from rabbit reticulocytes. J. biol. Chem. 244, 4330 (1969).

    PubMed  CAS  Google Scholar 

  190. Maden, B. E. H., Traut, R. R., Monro, R. E.: Ribosome-catalyzed peptidyl transfer: the polyphenylalanine system. J. molec. Biol. 35, 333 (1968).

    Article  PubMed  CAS  Google Scholar 

  191. Malkin, M., Lipmann, F.: Fusidic acid: inhibition of factor T2 in reticulocyte protein synthesis. Science 164, 71 (1969).

    Article  PubMed  CAS  Google Scholar 

  192. Mangiarotti, G., Schlessinger, D.: Polyribosome metabolism in E. coli. I. Extraction of polyribosomes and ribosomal subunits from fragile, growing E. coli. J. molec. Biol. 20, 123 (1966).

    Article  PubMed  CAS  Google Scholar 

  193. Mao, J. C.-H., Robishaw, E. E.: Effects of macrolides on peptide-bond formation and translocation. Biochemistry 10, 2054 (1971).

    Article  PubMed  CAS  Google Scholar 

  194. Marcus, A., Bewley, J. D., Weeks, D. P.: Aurintricarboxylic acid and initiation factors of wheat embryo. Science 167, 1735 (1970).

    Article  PubMed  CAS  Google Scholar 

  195. Matsua, S., Shiratori, O., Katagiri, K.: Antitumor activity of showdomycin. J. Antibiot. Ser. A (Tokyo) 17, 234 (1964).

    Google Scholar 

  196. Matthaei, H., Amelunxen, F., Eckert, K., Heller, G.: The mechanism of protein biosynthesis I. The binding of messenger-RNA and aminoacyl sRNA to ribosomes. Ber. Bunsengesellschaft 68, 735 (1964).

    Google Scholar 

  197. Maxwell, I. H.: Studies of the binding of tetracycline to ribosomes in vitro. Molec. Pharmacol. 4, 25 (1968).

    CAS  Google Scholar 

  198. Maxwell, R. E., Nickel, V. S. The antibacterial activity of the isomers of chloramphenicol. Antibiot. and Chemother. 4, 289 (1954).

    CAS  Google Scholar 

  199. Mazumder, R., Chae, Y.-B., Ochoa, S.: Polypeptide chain initiation in E. coli: suifhydryl groups and the function of initiation factor F2. Proc. nat. Acad. Sci. (Wash.) 63, 98 (1969).

    Article  CAS  Google Scholar 

  200. Miller, D. L., Hachmann, J., Weissbach, H.: The reaction of the sulfhydryl groups on the elongation factors Tu and Ts. Arch. Biochem. Biophys. 144, 115 (1971).

    Article  PubMed  CAS  Google Scholar 

  201. Miller, D. L., Weissbach, H.: An interaction between the transfer factors required for protein synthesis. Arch. Biochem. Biophys. 132, 146 (1969).

    Article  PubMed  CAS  Google Scholar 

  202. Miller, D. L., Weissbach, H.: Studies on the purification and properties of factor Tu from E. coli. Arch. Biochem. Biophys. 141, 26 (1970).

    Article  CAS  Google Scholar 

  203. Miller, M. J., Zasloff, M., Ochoa, S.: Association of polypeptide initiation factors with 30 S ribosomal subunits. FEBS Letters 3, 50 (1969).

    Article  PubMed  CAS  Google Scholar 

  204. Milman, G., Goldstein, J., Scolnick, E., Caskey, T.: Peptide chain termination III. Stimulation of in vitro termination. Proc. nat. Acad. Sci. (Wash.) 63, 183 (1969).

    Article  CAS  Google Scholar 

  205. Miskin, R., Zamir, A., Elson, D.: Inactivation and reactivation of ribosomal subunits s: the peptidyl transferase activity of the 50 S subunit of E. coli. J. molec. Biol. 54, 355(1970).

    Article  PubMed  CAS  Google Scholar 

  206. Mizushima, S., Nomura, M.: Assembly mapping of 30 S ribosomal proteins from E. coli. Nature (Loud.) 226, 1214 (1970).

    Article  CAS  Google Scholar 

  207. Modolell, J.: Int. Cong. Biochem. 8th (Switz.) (1970).

    Google Scholar 

  208. Modolell, J., Cabrer, B., Parmeggiani, A., Vazquez, D.: Inhibition by siomycin and thiostrepton of both aminoacyl-tRNA and factor G binding to ribosomes. Proc. nat. Acad. Sci. (Wash.) 68, 1796 (1971).

    Article  CAS  Google Scholar 

  209. Modolell, J., Davis, B.: Rapid inhibition of polypeptide chain extension by streptomycin. Proc. nat. Acad. Sci. (Wash.) 61, 1279 (1968).

    Article  CAS  Google Scholar 

  210. Modolell, J., Davis, B. D.: A unitary mechanism for the several effects of streptomycin on the ribosome. Cold Spr. Harb. Symp. quant. Biol. 34, 113 (1969).

    CAS  Google Scholar 

  211. Modolell, J., Davis, B. D.: Breakdown by streptomycin of initiation complexes formed on ribosomes of E. coli. Proc. nat. Acad. Sci. (Wash.) 67, 1148 (1970).

    Article  CAS  Google Scholar 

  212. Modolell, J., Vazquez, D., Monro, R. E.: Ribosomes, G-factor and siomycin. Nature (Lond.) New Biol. 230, 109 (1971).

    Article  CAS  Google Scholar 

  213. Monro, R. E.: Catalysis of peptide bond formation by 50 S ribosomal subunits from Escherichia coli. J. molec. Biol. 26, 147 (1967).

    Article  PubMed  CAS  Google Scholar 

  214. Monro, R. E., Celma, M. L., Vazquez, D.: Action of sparsomycin on ribosome-catalyzed peptidyl transfer. Nature (Lond.) 222, 356 (1969).

    Article  CAS  Google Scholar 

  215. Monro, R. E., Marcker, K. A.: Ribosome-catalyzed reaction of puromycin with a formylmethionine-containing oligonucleotide. J. molec. Biol. 25, 347 (1967).

    Article  PubMed  CAS  Google Scholar 

  216. Monro, R. E., Cerna, J., Marcker, K. A.: Ribosome-catalyzed peptidyl transfer: substrate specificity at the P-site. Proc. nat. Acad. Sci. (Wash.) 61, 1042 (1968).

    Article  CAS  Google Scholar 

  217. Monro, R. E., Vazquez, D.: Ribosome-catalyzed peptidyl transfer: effects of some inhibitors of protein synthesis. J. molec. Biol. 28, 161 (1967).

    Article  PubMed  CAS  Google Scholar 

  218. Moore, C. H., Farron, F., Bohnert, D., Weissmann, C.: Possible origin of a minor virus specific protein (a1 in Qβ particles. Nature (Lond.) New Biol. 234, 204 (1971).

    CAS  Google Scholar 

  219. Morse, D.: Polarity induced by chloramphenicol and relief by suA. J. molec. Biol. 55, 113 (1971).

    Article  PubMed  CAS  Google Scholar 

  220. Nakamoto, T.: The initial phase in the polyuridylic acid-directed polymerization of phenylalanine. J. biol. Chem. 242, 4534 (1967).

    PubMed  CAS  Google Scholar 

  221. Nathans, D.: Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains. Proc. nat. Acad. Sci. (Wash.) 51, 585 (1964).

    Article  CAS  Google Scholar 

  222. Nathans, D., Neidle, P.: Structural requirements for puromycin inhibition of protein synthesis. Nature (Lond.) 197, 1076 (1963).

    Article  CAS  Google Scholar 

  223. Nathans, D., von Ehrenstein, G., Monro, R., Lipmann, F.: Protein synthesis from aminoacyl soluble ribonucleic acid. Fed. Proc. 21, 127 (1962).

    PubMed  CAS  Google Scholar 

  224. Neth, R., Monro, R. E., Heller, G., Battaner, E., Vazquez, D.: Catalysis of peptidyl transfer by human tonsil ribosomes and effects of some antibiotics. FEBS Letters 6, 198 (1970).

    Article  PubMed  CAS  Google Scholar 

  225. Nichols, J. L.: Nucleotide sequence from the polypeptide chain termination region of the coat protein cistron in bacteriophage R17 RNA. Nature (Lond.) 225, 147 (1970).

    Article  CAS  Google Scholar 

  226. Nirenberg, W. M., Leder, P.: RNA codewords and protein synthesis. Science 145, 1399 (1964).

    CAS  Google Scholar 

  227. Nirenberg, W. M., Leder, P., Bernfield, M., Brimacombe, R., Trupin, J., Rottman, F., O’Neal, C.: RNA codewords and protein synthesis. VII. On the general nature of the RNA code. Proc. nat. Acad. Sci. (Wash.) 53, 1161 (1965).

    Article  CAS  Google Scholar 

  228. Nirenberg, M. W., Matthaei, J. H.: The dependence of cell-free protein synthesis upon naturally occurring or synthetic polyribonucleotides. Proc. nat. Acad. Sci. (Wash.) 47, 1588 (1961).

    Article  CAS  Google Scholar 

  229. Nishimura, H., Mayama, M., Komatsu, Y., Kato, H., Shimaoka, N., Tanaka, Y.: Showdomycin: a new antibiotic from a Streptomyces SP. J. Antibiot. Ser. A (Tokyo) 17, 148 (1964).

    CAS  Google Scholar 

  230. Nishizuka, Y., Lipmann, F.: Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system. Proc. nat. Acad. Sci. (Wash.) 55, 212 (1966).

    Article  CAS  Google Scholar 

  231. Nishizuka, Y., Lipmann, F.: The interrelationship between GTP and amino acid polymerization. Arch. Biochem. Biophys. 116, 344 (1966).

    Article  PubMed  CAS  Google Scholar 

  232. Nomura, M., Lowry, C.: Phage F2 RNA-directed binding of formylmethionyl-tRNA to ribosomes and the role of 30 S ribosomal subunits in initiation of protein synthesis. Proc. nat. Acad. Sci. (Wash.) 58, 946 (1967).

    Article  CAS  Google Scholar 

  233. Nomura, M., Lowry, C. V., Guthrie, C.: The initiation of protein synthesis: joining of the 50 S ribosomal subunit to the initiation complex. Proc. nat. Acad. Sci. (Wash.) 58, 1487 (1967).

    Article  CAS  Google Scholar 

  234. Nomura, M., Mizushima, S., Ozaki, M., Traub, P., Lowry, C. V.: Structure and function of ribosomes and their molecular components. Cold Spr. Harb. Symp. quant. Biol. 34, 49 (1969).

    CAS  Google Scholar 

  235. Obrig, T. G., Culp, W. J., McKeehan, W. L., Hardesty, B.: The mechanism by which cyclobeximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes. J. biol. Chem. 246, 174 (1971).

    PubMed  CAS  Google Scholar 

  236. Obrig, T. G., Irvin, J., Culp, W., Hardesty, B.: Inhibition of peptide initiation on reticulocyte ribosomes by edeine. Europ. J. Biochem. 21, 31 (1971).

    Article  PubMed  CAS  Google Scholar 

  237. Ohta, T., Sarkar, S., Thach, R. E.: The role of guanosine 5’-triphosphate in the initiation of peptide synthesis. III. Binding of formylmethionyl-tRNA to ribosomes. Proc. nat. Acad. Sci. (Wash.) 58, 1638 (1967).

    Article  CAS  Google Scholar 

  238. Okura, A., Kinoshita, T., Tanaka, N.: Complex formation of fusidic acid with G-factor, ribosome and guanosine nucleotide. Biochem. biophys. Res. Commun. 41, 1545 (1970).

    Article  PubMed  CAS  Google Scholar 

  239. Okuyama, A., Machiyama, M., Kinoshita, T., Tanaka, N.: Inhibition by kasugamycin of initiation complex formation on 30 S ribosomes. Biochem. biophys. Res. Commun. 43, 196 (1971).

    Article  PubMed  CAS  Google Scholar 

  240. Old, D., Gorini, L.: Amino acid changes provoked by streptomycin in a polypeptide synthesized in vitro. Science 150, 1290 (1965).

    Article  PubMed  CAS  Google Scholar 

  241. Oleinick, N. L., Corcoran, J. W.: Two types of binding of erythromycin to sibosomes from antibiotic sensitive and resistant Bacillus subtilis 168. J. biol. Chem. 244, 727 (1969).

    PubMed  CAS  Google Scholar 

  242. Oleinick, N. L., Wilhelm, J. M., Corcoran, J. W.: Nonidentity of the site of action of erythromycin A and chloramphenicol on Bacillus subtilis ribosomes. Biochim. biophys. Acta (Amst.) 155, 290 (1968).

    CAS  Google Scholar 

  243. Ono, Y., Skoultchi, A., Klein, A., Lengyel, P.: Peptide chain elongation: discrimination against the initiator transfer RNA by microbial amino acid polymerization factors. Nature (Lond.) 220, 1304 (1968).

    Article  CAS  Google Scholar 

  244. Ono, Y., Skoultchi, A., Waterson, J., Lengyel, P.: Stoichiometry of aminoacyl-transfer RNA binding and GTP during chain elongation and translocation. Nature (Lond.) 223, 697 (1969).

    Article  CAS  Google Scholar 

  245. Ono, Y., Skoultchi, A., Waterson, J., Lengyel, P.: Peptide chain elongation: GTP cleavage catalyzed by factors binding aminoacyl-transfer RNA to the ribosome. Nature (Lond.) 222, 645 (1969).

    Article  CAS  Google Scholar 

  246. Otaka, E., Teraoka, H., Tamaki, M., Tanaka, K., Osawa, S.: Ribosomes from erythromycin resistant mutants of E. coli Q13. J. molec. Biol. 48, 499 (1970).

    Article  PubMed  CAS  Google Scholar 

  247. Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin resistant locus in E. coli. Nature (Lond.) 222, 333 (1969).

    Article  CAS  Google Scholar 

  248. Panet, A., Degroot, N., Lapidot, Y.: Substrate specificity of E. coli peptidyl transferase. Europ. J. Biochem. 15, 222 (1970).

    Article  PubMed  CAS  Google Scholar 

  249. Parenti-Rosina, R., Eisenstadt, A., Eisenstadt, J. M.: Isolation of protein initiation factors from 30 S ribosomal subunits. Nature (Lond.) 221, 363 (1969).

    Article  CAS  Google Scholar 

  250. Parmeggiani, A.: Crystalline transfer factors from E. coli. Biochem. biophys. Res. Commun. 30, 613 (1968).

    Article  PubMed  CAS  Google Scholar 

  251. Parmeggiani, A., Gottschalk, E. M.: Isolation and some properties of the amino acid polymerization factors from Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 34, 377 (1969).

    CAS  Google Scholar 

  252. Parmeggiani, A., Gottschalk, E. M.: Properties of the crystalline amino acid polymerization factors from Escherichia coli: binding of G to ribosomes. Biochem. biophys. Res. Commun. 35, 861 (1969).

    Article  PubMed  CAS  Google Scholar 

  253. Pavan, M., Bo, G.: Pederin, toxic principle obtained in the crystalline state from the beetle Paederus fuscipes curt. Physiol, comp. (’s-Grav.) 3, 307 (1953).

    CAS  Google Scholar 

  254. Perani, A., Parisi, B., Decarli, L., Ciferri, O.: Incorporation of amino acids by a cell-free system prepared from human cells cultured in vitro, Biochim. biophys. Acta (Amst.) 161, 223 (1968).

    CAS  Google Scholar 

  255. Pestka, S.: The action of streptomycin on protein synthesis in vitro. Bull. N. Y. Acad. Med. 43, 126 (1967).

    PubMed  CAS  Google Scholar 

  256. Pestka, S.: Inhibitors of ribosome functions. Ann. Rev. Microbiol. 25, 487 (1971).

    Article  CAS  Google Scholar 

  257. Pestka, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. V. On the function of a soluble transfer factor in protein synthesis. Proc. nat. Acad. Sci. (Wash.) 61, 726 (1968).

    CAS  Google Scholar 

  258. Pestka, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. VIII. Survey of the effect of antibiotics on N-acetyl-phenylalanyl-puromycin formation: possible mechanism of chloramphenicol action. Arch. Biochem. Biophys. 136, 80 (1970).

    Article  PubMed  CAS  Google Scholar 

  259. Pestka, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. IX. Effect of antibiotics on translocation and peptide bond formation. Arch. Biochem. Biophys. 136, 89 (1970).

    Article  PubMed  CAS  Google Scholar 

  260. Pestka, S.: Studies on the formation of transfer ribonucleic acid-ribosone complexes. X. Phenylalanyl-oligonucleotide binding to ribosomes and the mechanism of chloramphenicol action. Biochem. biophys. Res. Commun. 36, 589 (1969).

    Article  PubMed  CAS  Google Scholar 

  261. Pestka, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomes. Proc. nat. Acad. Sci. (Wash.) 64, 709 (1969).

    Article  CAS  Google Scholar 

  262. Pestka, S.: Translocation, aminoacyl-oligonucleotides, and antibiotic action. Cold Spr. Harb. Symp. quant. Biol. 34, 395 (1969).

    CAS  Google Scholar 

  263. Pestka, S., Hishizawa, T., Lessard, J. L.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. XIII. Aminoacyl oligonucleotide binding to ribosomes: characteristics and requirements. J. biol. Chem. 245, 6208 (1970).

    PubMed  CAS  Google Scholar 

  264. Pestka, S., Marshall, R., Nirenberg, M.: RNA codewords and protein synthesis. V. Effect of streptomycin on the formation of ribosome-sRNA complexes. Proc. nat. Acad. Sci. (Wash.) 53, 639 (1965).

    Article  CAS  Google Scholar 

  265. Pestka, S., Nirenberg, M. W.: Regulatory mechanisms and protein synthesis. X. Codon recognition on 30 S ribosomes. J. molec. Biol. 21, 145 (1966).

    Article  PubMed  CAS  Google Scholar 

  266. Phillips, L. A., Hotham-Iglewski, B., Franklin, R. M.: Ribosomes of Escherichia coli. I. Effzcts of monovalent cations on the distribution of polysomes, ribosomes and ribosomal subunits. J. molec. Biol. 40, 279 (1969).

    Article  PubMed  CAS  Google Scholar 

  267. Plotz, P. H., Davis, B. D.: Absence of chloramphenicol-insensitive phase of streptomycin action. J. Bact. 83, 802 (1962).

    PubMed  CAS  Google Scholar 

  268. Prichard, P. M., Gilbert, J. M., Shafritz, D. A., Anderson, W. F.: Factors for the initiation of haemoglobin synthesis by rabbit reticulocyte ribosomes. Nature (Lond.) 226, 511 (1970).

    Article  CAS  Google Scholar 

  269. Raeburn, S., Goor, R. S., Schneider, J. A., Maxwell, E. S.: Interaction of aminoacyl transferase II and guanosine triphospbate: inhibition by diphtheria toxin and nicotinamide adenine dinucleotide. Proc. nat. Acad. Sci. (Wash.) 61, 1428 (1968).

    Article  CAS  Google Scholar 

  270. Rao, P., Moldave, K.: The binding of aminoacyl sRNA and GTP to transferase I. Biochem. biophys. Res. Commun. 28, 909 (1967).

    Article  PubMed  CAS  Google Scholar 

  271. Rao, P.,Moldave, K.: Interaction of polypeptide chain elongation factors with rat liver ribosomal subunits. J. molec. Biol. 46, 447 (1969).

    Article  PubMed  CAS  Google Scholar 

  272. Rao, S. S., Grollman, A. P.: Cycloheximide resistance in yeast: a property of the 60 S ribosomal subunit. Biochem. biophys. Res. Commun. 29, 696 (1967).

    Article  PubMed  CAS  Google Scholar 

  273. Ravel, J. M., Shorey, R. L., Garner, C. W., Dawkins, R. C., Shive, W.: The role of an aminoacyl tRNA-GTP-protein complex in polypeptide synthesis. Cold Spr. Harb. Symp. quant. Biol. 34, 321 (1969).

    CAS  Google Scholar 

  274. Ravel, J. M., Shorey, R. L., Shive, W.: The composition of the active intermediate in the transfer of aminoacyl-RNA to ribosomes. Biochem. biophys. Res. Commun. 32, 9 (1968).

    Article  PubMed  CAS  Google Scholar 

  275. Ravel, J. M., Shorey, R. L., Shive, W.: Relationship between peptidyl transferase activity and interaction of ribosomes with phenylalanyl transfer ribonucleic acid-guanosine 5’-triphosphate TIu complex. Biochemistry 9, 5028 (1970).

    Article  PubMed  CAS  Google Scholar 

  276. Revel, M., Greenshpan, H., Herzberg, M.: Specificity in the binding of E. coli ribosomes to natural messenger RNA. Europ. J. Biochem. 16, 117 (1970).

    Article  PubMed  CAS  Google Scholar 

  277. Revel, M., Herzberg, M., Becarevic, A., Gros, F.: Role of a protein factor in the functional binding of ribosomes to natural messenger RNA. J. molec. Biol. 33, 231 (1968).

    Article  PubMed  CAS  Google Scholar 

  278. Revel, M., Herzberg, M., Greenshpan, H.: Initiator protein dependent binding of messenger RNA to the ribosome. Cold Spr. Harb. Symp. quant. Biol. 34, 261 (1969).

    CAS  Google Scholar 

  279. Revel, M., Lelong, J. C., Brawerman, G., Gros, F.: Function of three protein factors and ribosomal subunits in the initiation of protein synthesis in E. coli. Nature (Lond.) 219, 1016 (1968).

    Article  CAS  Google Scholar 

  280. Richter, D.: Formation of a ternary complex between yeast aminoacyl-tRNA binding factor, GTP, and aminoacyl-tRNA. Biochem. biophys. Res. Commun. 38, 864 (1970).

    Article  PubMed  CAS  Google Scholar 

  281. Richter, D., Lin, L., Bodley, J. W.: Studies on translocation IX: the pattern of action of antibiotic translocation inhibitors in eukaryotic and prokaryotic systems. Arch. Biochem. Biophys. 147, 186 (1971).

    Article  PubMed  CAS  Google Scholar 

  282. Roberts, W. K., Coleman, W. H.: Polyuridylic acid binding by protein from Ehrlich ascites cell ribosomes and its inhibition by Aurintricarboxylic acid. Biochemistry 10, 4304 (1971).

    Article  PubMed  CAS  Google Scholar 

  283. Ron, E. Z., Köhler, R. E., Davis, B. D.: Magnesium ion dependence of free and polysomal ribosomes from Escherichia coli. J. molec. Biol. 36, 83 (1968).

    Article  PubMed  CAS  Google Scholar 

  284. Rosset, R., Gorini, L.: A ribosomal ambiguity mutation. J. molec. Biol. 39, 95 (1969).

    Article  PubMed  CAS  Google Scholar 

  285. Roufa, D. J., Doctor, B. P., Leder, P.: The two site model of ribosomal function: a test using degenerate serine codons in bacteriophage f2 mRNA. Biochem. biophys. Res. Commun. 39, 231 (1970).

    Article  PubMed  CAS  Google Scholar 

  286. Rychlik, I., Cerna, C., Stanislav, C., Pulkrapek, P., Zemlicka, J.: Substrate specificity of ribosomal peptidyl transferase. The effect of the nature of the amino acid side chain on the acceptor activity of 2′(3′)-0-amino acyladenosines. Europ. J. Biochem. 16, 136 (1970).

    Article  PubMed  CAS  Google Scholar 

  287. Rychlik, I., Chladek, S., Zemlicka, J.: Release of peptide chains from the polylysyl-tRNA ribosome complex by cytidylyl-(3′-5′)-2′(3,)-0-glycyladenosine. Biochim. biophys. Acta (Amst.) 138, 640 (1967).

    CAS  Google Scholar 

  288. Sabol, S., Ochoa, S.: Ribosomal binding of labeled initiation factor F3. Nature (Lond.) New Biol. 234, 233 (1971).

    CAS  Google Scholar 

  289. Salas, M., Hille, M. B., Last, J. A., Wahba, A. J., Ochoa, S.: Translation of the genetic message, II. Effect of initiation factors on the binding of formylmethionyl tRNA to ribosomes. Proc. nat. Acad. Sci. (Wash.) 57, 387 (1967).

    Article  CAS  Google Scholar 

  290. Salas, M., Miller, M. J., Wahba, A. J., Ochoa, S.: Translation of the genetic message. V. Effect of Mg++ an formylation of methionine in protein synthesis. Proc. nat. Acad. Sci. (Wash.) 57, 1865 (1967).

    Article  CAS  Google Scholar 

  291. Sarkar, S., Thach, R. E.: Inhibition of formylmethionyl transfer RNA binding to ribosomes by tetracycline. Proc. nat. Acad. Sci. (Wash.) 60, 1479 (1968).

    Article  CAS  Google Scholar 

  292. Scaife, J., Beckwith, J. R.: Mutational alteration of the maximal level of lac Operon expression. Cold Spr. Harb. Symp. quant. Biol. 31, 403 (1966).

    CAS  Google Scholar 

  293. Schedl, P., Singer, R. E., Conway, T. W.: A factor required for the translation of bacteriophage f2 RNA in extracts of T4-infected cells. Biochem. biophys. Res. Commun. 38, 631 (1970).

    Article  PubMed  CAS  Google Scholar 

  294. Schlessinger, D., Mangiarotti, G., Apirion, D.: The formation and stabilization of 30 S and 50 S ribosome couples in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 58, 1782 (1967).

    Article  CAS  Google Scholar 

  295. Schlessinger, D., Gurgo, C., Luzzatto, L., Apirion, D.: Polyribosome metabolism in growing and non-growing E. coli. Cold Spr. Harb. Symp. quant. Biol. 34, 231 (1969).

    CAS  Google Scholar 

  296. Schneir, M., Moldave, K.: The isolation and biological activity of multiple forms of aminoacyl transferase I of rat liver. Biochim. biophys. Acta (Amst.) 166, 58 (1968).

    CAS  Google Scholar 

  297. Schreier, M. H., Noll, H.: Conformational changes in ribosomes during protein synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 805 (1971).

    Article  CAS  Google Scholar 

  298. Scolnick, E. M., Caskey, C. T.: Peptide chain termination. V. The role of release factors in mRNA terminator codon recognition. Proc. nat. Acad. Sci. (Wash.) 64, 1235 (1969).

    Article  CAS  Google Scholar 

  299. Scolnick, E. M., Tompkins, R., Caskey, T., Nirenberg, M.: Release factors differing in specificity for terminator codons. Proc. nat. Acad. Sci. (Wash.) 61, 768 (1968).

    Article  CAS  Google Scholar 

  300. Shafritz, D. A., Anderson, W. F.: Factor dependent binding of methionyl-tRNAs to reticulocyte ribosomes. Nature (Lond.) 227, 918 (1970).

    Article  CAS  Google Scholar 

  301. Shafritz, D. A., Laycock, D. G., Crystal, R. G., Anderson, W. F.: Requirement for GTP in the initiation process on reticulocyte ribosomes and ribosomal subunits. Proc. nat. Acad. Sci. (Wash.) 68, 2246 (1971).

    Article  CAS  Google Scholar 

  302. Shafritz, D. A., Prichard, P. M., Gilbert, J. M., Anderson, W. F.: Separation of two factors, M1 and M2, required for poly U dependent polypeptide synthesis by rabbit reticulocyte ribosomes at low magnesium ion concentration. Biochem. biophys. Res. Commun. 38, 721 (1970).

    Article  PubMed  CAS  Google Scholar 

  303. Sherman, M. I., Simpson, M. V.: The role of ribosomal conformation in protein biosynthesis: the streptomycin-ribosome interaction. Proc. nat. Acad. Sci. (Wash.) 64, 1388 (1969).

    Article  CAS  Google Scholar 

  304. Shorey, R. L., Ravel, J. M., Garner, C. W., Shive, W.: Formation and properties of the aminoacyl transfer ribonucleic acid-guanosine triphosphate-protein complex. An intermediate in the binding of aminoacyl transfer ribonucleic acid. J. biol. Chem. 244, 4555 (1969).

    PubMed  CAS  Google Scholar 

  305. Siegelman, F. L., Apirion, D.: Inhibition of polyuridylic acid-directed protein synthesis by aurintricarboxylate in extracts of Escherichia coli. J. Bact. 105, 451 (1971).

    CAS  Google Scholar 

  306. Sisler, J., Moldave, K.: Studies on the binding of phenylalanyl transfer RNA to rat-liver ribosomes. Biochim. biophys. Acta (Amst.) 195, 123 (1969).

    Google Scholar 

  307. Skogerson, L., Roufa, D., Leder, P.: Characterization of the initial peptide of Qβ RNA polymerase and control of its synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 276 (1971).

    Article  CAS  Google Scholar 

  308. Skoultchi, A., Ono, Y., Waterson, J., Lengyel, P.: Peptide chain elongation. Cold Spr. Harb. Symp. quant. Biol. 34, 437 (1969).

    CAS  Google Scholar 

  309. Skoultchi, A., Ono, Y., Moon, H. M., Lengyel, P.: On three complementary amino acid polymerization factors from Bacillus stearothermophilus separation of a complex containing two of the factors, guanosine-5’-triphosphate and aminoacyl transfer RNA. Proc. nat. Acad. Sci. (Wash.) 60, 675 (1968).

    Article  CAS  Google Scholar 

  310. Skoultchi, A., Ono, Y., Waterson, J., Lengyel, P.: Peptide chain elongation: indications for the binding of an amino acid polymerization factor, guanosine 5’-triphosphate-aminoacyl transfer ribonucleic acid complex to the messenger ribosome complex. Biochemistry 9, 508 (1970).

    Article  PubMed  CAS  Google Scholar 

  311. Slechta, L.: Mode of action of sparsomycin in Escherichia coli. Antimicrobial Agent Chemotherapy 326 (1965).

    Google Scholar 

  312. Smith, A. E., Marcker, K. A.: Cytoplasmic methionine transfer RNA’s from eukaryotes. Nature (Lond.) 226, 607 (1970).

    Article  CAS  Google Scholar 

  313. Sparling, P. F., Modolell, J., Takeda, Y., Davis, B. D.: Ribosomes from Escherichia coli merodiploids heterozygous for resistance to streptomycin and to spectinomycin. J. molec. Biol. 37, 407 (1968).

    Article  PubMed  CAS  Google Scholar 

  314. Spirin, A. S.: How does the ribosome work? A hypothesis based on the two subunit construction of the ribosome. Curr. modern Biol. 2, 115 (1968).

    CAS  Google Scholar 

  315. Spyrides, G. J., Lipmann, F.: The effect of univalent cations on the binding of sRNA to the template-ribosome complex. Proc. nat. Acad. Sci. (Wash.) 51, 1220 (1964).

    Article  CAS  Google Scholar 

  316. Staehelin, T., Maglotti, D., Monro, R. E.: On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure. Cold Spr. Harb. Symp. quant. Biol. 34, 39 (1969).

    CAS  Google Scholar 

  317. Steitz, J. A.: Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature (Lond.) 224, 957 (1969).

    Article  CAS  Google Scholar 

  318. Steitz, J. A.,Dube, S. K., Rudland, P. S.: Control of translation by T4 phage: altered ribosome binding at R17 initiation sites. Nature (Lond.) 226, 824 (1970).

    Article  CAS  Google Scholar 

  319. Stern, J. L., Cohen, S. S.: Lethality and the stimulation of RNA synthesis by streptomycin. Proc. nat. Acad. Sci. (Wash.) 51, 859 (1964).

    Article  CAS  Google Scholar 

  320. Stewart, M. L., Grollman, A. P., Huang, M.-T.: Aurintricarboxylic acid: inhibitor of initiation of protein synthesis. Proc. nat. Acad. Sci. (Wash.) 68, 97 (1971).

    Article  CAS  Google Scholar 

  321. Stewart-Blair, M. L., Yanowitz, I. S., Goldberg, I. H.: Inhibition of synthesis of new globin chains in reticulocyte lysates by pactamycin. Biochemistry 10, 4198 (1971).

    Article  PubMed  CAS  Google Scholar 

  322. Stretton, A. O. W., Brenner, S.: Molecular consequences of the amber mutation and its supression. J. molec. Biol. 12, 456 (1965).

    Article  PubMed  CAS  Google Scholar 

  323. Suarez, G., Nathans, D.: Inhibition of aminoacyl-sRNA binding to ribosomes by tetracycline. Biochem. biophys. Res. Commun. 18, 743 (1965).

    Article  CAS  Google Scholar 

  324. Subramanian, A. R., Davis, B. D.: Rapid exchange of subunits between free ribosomes in extracts of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 68, 2453 (1971).

    Article  CAS  Google Scholar 

  325. Subramanian, A. R., Davis, B. D., Beller, R. J.: The ribosome dissociation factor and the ribosome-polysome cycle. Cold Spr. Harb. Symp. quant. Biol. 34, 223 (1969).

    CAS  Google Scholar 

  326. Suzuka, I., Kaji, A.: Studies on diphenylalanine synthesis. Biochim. biophys. Acta (Amst.) 149, 540 (1967).

    CAS  Google Scholar 

  327. Suzuka, I., Kaji, H., Kaji, A.: Binding of specific sRNA to 30 S ribosomal subunits: effect of 50 S ribosomal subunits. Proc. nat. Acad. Sci. (Wash.) 55, 1483 (1966).

    Article  CAS  Google Scholar 

  328. Suzuka, I., Kaji, H., Kaji, A.: Binding of specific sRNA to 30 S-subunits. Comparison with the binding to 70 S ribosomes. Biochem. biophys. Res. Commun. 21, 187 (1965).

    Article  PubMed  CAS  Google Scholar 

  329. Swan, D., Matthaei, H.: Further evidence for the four-site model of ribosomal function: bacteriophage fr mRNA coded binding of aa-tRNA. FEBS Letters 17, 215 (1971).

    Article  PubMed  CAS  Google Scholar 

  330. Symons, R. H., Harris, R. J., Clarke, L. P., Wheldrake, J. F., Elliott, W. H.: Structural requirements for inhibition of polyphenylalanine synthesis by aminoacyl and nucleotidyl analogues of puromycin. Biochim. biophys. Acta (Amst.) 179, 248 (1969).

    CAS  Google Scholar 

  331. Szer, W., Kurylo-Borowska, Z.: Effect of edeine on aminoacyl-tRNA binding to ribosomes and its relationship to ribosomal binding sites. Biochim. biophys. Acta (Amst.). 224, 477 (1970).

    CAS  Google Scholar 

  332. Takanami, M., Okamoto, T.: Interaction of ribosomes and synthetic polyribonucleotides. J. molec. Biol. 7, 323 (1963).

    Article  PubMed  CAS  Google Scholar 

  333. Tanaka, K., Teraoka, H., Tamaki, M.: Peptidyl puromycin synthesis: effect of several antibiotics which act on 50 S ribosomal subunits. FEBS Letters 13, 65 (1971).

    Article  PubMed  CAS  Google Scholar 

  334. Tanaka, K., Watanabe, S., Teraoka, H., Tamaki, M.: Effect of siomycin on protein synthesizing activity of Escherichia coli ribosomes. Biochem. biophys. Res. Commun. 39, 1189 (1970).

    Article  PubMed  CAS  Google Scholar 

  335. Tanaka, K., Watanabe, S., Tamaki, M.: Mode of action of siomycin. J. Antibiot. Ser. A (Tokyo) 23, 13 (1970).

    CAS  Google Scholar 

  336. Tanaka, N., Kawano, G., Kinoshita, T.: Chromosomal location of a fusidic acid resistant marker in Escherichia coli. Biochem. biophys. Res. Commun. 42, 564 (1971).

    Article  PubMed  CAS  Google Scholar 

  337. Tanaka, N., Lin, Y.-C., Okuyama, A.: Studies on translocation of f-Met-tRNA and peptidyl tRNA with antibiotics. Biochem. biophys. Res. Commun. 44, 477 (1971).

    Article  PubMed  CAS  Google Scholar 

  338. Tanaka, N., Masukawa, H., Umezawa, H.: Structural basis of kanamycin for miscoding activity. Biochem. biophys. Res. Commun. 26, 544 (1967).

    Article  PubMed  CAS  Google Scholar 

  339. Tanaka, S., Igarashi, K., Kaji, A.: Further studies on the action of puromycin and tetracycline. J. biol. Chem. (1972) (in press).

    Google Scholar 

  340. Tanaka, S., Kaji, A.: Does translocase (G-factor) require the presence of unesterified tRNA on the donor site for its action? The effect of fusidic acid. Biochem. biophys. Res. Commun. 46, 136 (1972).

    Article  PubMed  CAS  Google Scholar 

  341. Tanaka, Y., Kaji, H.: The role of ribosomal protein for the binding of dihydrostreptomycin to ribosomes. Biochem. biophys. Res. Commun. 32, 313 (1968).

    Article  PubMed  CAS  Google Scholar 

  342. Teraoka, H.: Reversal of the inhibitory action of chloramphenicol on the ribosomal peptidyl transfer reaction by erythromycin. Biochim. biophys. Acta (Amst.) 213, 535 (1970).

    CAS  Google Scholar 

  343. Teraoka, H., Tanaka, K., Tamaki, M.: The comparative study on the effects of chloramphenicol, erythromycin and lincomycin on polylysine synthesis in an Escherichia coli cell-free system. Biochim. biophys. Acta (Aunt.) 174, 776 (1969).

    CAS  Google Scholar 

  344. Thach, S. S., Thach, R. E.: Translocation of messenger RNA and “accomodation” of fMet-tRNA. Proc. nat. Acad. Sci. (Wash.) 68, 1791 (1971).

    Article  CAS  Google Scholar 

  345. Thach, S. S., Thach, R. E.: One molecule of guanosine triphosphate is present in each 30 S initiation complex. Nature (Lond.) New Biol. 229, 219 (1971).

    Article  CAS  Google Scholar 

  346. Tocchini-Valentinl, G. P., Felicetti, L., Rinaldi, G. M.: Mutants of Escherichia coli blocked in protein synthesis: mutants with an altered G-factor. Cold Spr. Harb. Symp. quant. Biol. 34, 463 (1969).

    Google Scholar 

  347. Tompkins, R. K.,Scolnick, E. M., Caskey, C. T.: Peptide chain termination. VII. The ribosomal and release factor requirements for peptide release. Proc. nat. Acad. Sci. (Wash.) 65, 702 (1970).

    Article  CAS  Google Scholar 

  348. Traub, P., Nomura, M.: Structure and function of E. coli ribosomes. V. Reconstruction of functionally active 30 S ribosomal particles from RNA and proteins. Proc. nat. Acad. Sci. (Wash.) 59, 777 (1968).

    Article  CAS  Google Scholar 

  349. Vanderhaeghe, H., van Dyck, P., Parmentier, G., Desomer, P.: Isolation and properties of the components of staphylomycin. Antibiot. and Chemother. 7, 606 (1957).

    CAS  Google Scholar 

  350. Vazquez, D.: Antibiotics which affect protein synthesis: the uptake of 14C-chloramphenicol by bacteria. Biochem. biophys. Res. Commun. 12, 409 (1963).

    Article  PubMed  CAS  Google Scholar 

  351. Vazquez, D.: Binding of CM to ribosomes: the effect of a number of antibiotics. Biochim. biophys. Acta (Amst.) 114, 277 (1966).

    CAS  Google Scholar 

  352. Vazquez, D.: Antibiotics affecting chloramphenicol uptake by bacteria. Their effect on amino acid incorporation in a cell-free system. Biochim. biophys. Acta (Amst.) 114, 289 (1966).

    CAS  Google Scholar 

  353. Vazquez, D.: Studies on the mode of action of the streptomycin antibiotics. J. gen. Microbiol. 42, 93 (1966).

    PubMed  CAS  Google Scholar 

  354. Vazquez, D., Battane, E., Neth, R. R., Heller, G., Monro, R. E.: The function of 80 S ribosomal subunits and effects of some antibiotics. Cold Spr. Harb. Symp. quant. Biol. 34, 369 (1969).

    CAS  Google Scholar 

  355. Vazquez, D., Monro, R. E.: Effects of some inhibitors of protein synthesis on the binding of aminoacyl tRNA to ribosomal subunits. Biochim. biophys. Acta (Amst.) 142, 155 (1967).

    CAS  Google Scholar 

  356. Vermeer, C., Talens, J., Bloemsma-Jonkman, F., Bosch, L.: Studies on the ribosomal initiation factor F3. Multiplicity of F3 and DF activities. FEBS Letters 19, 201 (1971).

    Article  PubMed  CAS  Google Scholar 

  357. Vogel, Z., Vogel, T., Elson, D.: The effect of erythromycin on peptide bond formation and the termination reaction. FEBS Letters 15, 249 (1971).

    Article  PubMed  CAS  Google Scholar 

  358. Vogel, Z., Vogel, T., Zamir, A., Elson, D.: Ribosome activation and the binding of dihydrostreptomycin: effect of polynucleotides and temperature on activation. J. molec. Biol. 54, 379 (1970).

    Article  PubMed  CAS  Google Scholar 

  359. Vogel, Z., Zamir, A., Elson, D.: The possible involvement of peptidyl transferase in the termination step of protein synthesis. Biochemistry 8, 5161 (1969).

    Article  PubMed  CAS  Google Scholar 

  360. Wahba, J. A., Chae, Y.-B., Iwasaki, K., Mazumder, R., Miller, M. J., Sabol, S., Sillero, M. A. G.: Initiation of protein synthesis in Escherichia coli. I. Purification and properties of the initiation factors. Cold Spr. Harb. Symp. quant. Biol. 34, 285 (1969).

    CAS  Google Scholar 

  361. Waller, J.-P., Erdos, T., Lemoine, F., Guttmann, S., Sandrin, E.: Inhibition of protein synthesis by aminoacyl 3′(2′)-adenosine. Biochim. biophys. Acta (Amst.) 119, 566 (1966).

    CAS  Google Scholar 

  362. Warner, J. R., Rich, A.: The number of soluble RNA molecules on reticulocyte polyribosomes. Proc. nat. Acad. Sci. (Wash.) 51, 1134 (1964).

    Article  CAS  Google Scholar 

  363. Watanabe, S., Tanaka, K.: Effect of siomycin on the acceptor site of Escherichia coli ribosomes. Biochem. biophys. Res. Commun. 45, 728 (1971).

    Article  PubMed  CAS  Google Scholar 

  364. Waterson, J., Beaud, G., Lengyel, P.: The S1 factor in peptide chain elongation. Nature (Lond.) 227, 34 (1970).

    Article  CAS  Google Scholar 

  365. Weber, M. J., Demoss, J. A.: The inhibition by chloramphenicol of nascent protein formation in E. coli. Proc. nat. Acad. Sci. (Wash.) 55, 1224 (1966).

    Article  CAS  Google Scholar 

  366. Weber, M. J., Demoss, J. A.: Inhibition of the peptide bond syntheizing cycle by chloramphenicol. J. Bact. 97, 1099 (1969).

    PubMed  CAS  Google Scholar 

  367. Webster, R. E., Zinder, N. D.: Fate of the message-ribosome complex upon translation of termination signals. J. molec. Biol. 42, 425 (1969).

    Article  PubMed  CAS  Google Scholar 

  368. Weisblum, B., Davies, J.: Antibiotic inhibitors of the bacterial ribosome. Bact. Rev. 32, 493 (1968).

    PubMed  CAS  Google Scholar 

  369. Weisblum, B., Demohn, V.: Inhibition by thiostrepton of the formation of a ribosome-bound guanine nucleotide complex. FEBS Letters 11, 149 (1970).

    Article  PubMed  CAS  Google Scholar 

  370. Weissbach, H., Brot, N.: Inhibition of transfer factor Ts by aurintricarboxylic acid. Biochem. biophys. Res. Commun. 39, 1194 (1970).

    Article  PubMed  CAS  Google Scholar 

  371. Weissbach, H., Kurylo-Borowska, Z., Szer, W.: Effect of edeine on aminoacyl tRNA binding to ribosomes. Arch. Biochem. Biophys. 146, 356 (1971).

    Article  PubMed  CAS  Google Scholar 

  372. Weissbach, H., Redfield, B., Brot, N.: Further studies on the role of factors Ts and Tu in protein synthesis. Arch. Biochem. Biophys. 144, 224 (1971).

    Article  PubMed  CAS  Google Scholar 

  373. Weissbach, H., Redfield, B., Brot, N.: Aminoacyl tRNA-Tu-GTP interaction with ribosomes. Arch. Biochem. Biophys. 145, 676 (1971).

    Article  PubMed  CAS  Google Scholar 

  374. Weissbach, H., Redfield, B., Hachman, J.: Studies on the role of factor Ts in aminoacyl-tRNA binding to ribosomes. Arch. Biochem. Biophys. 141, 384 (1970).

    Article  PubMed  CAS  Google Scholar 

  375. Wettstein, F. O., Noll, H.: Binding of transfer ribonucleic acid to ribosomes engaged in protein synthesis: number and properties of ribosomal binding sites. J. molec. Biol. 11, 35 (1965).

    Article  PubMed  CAS  Google Scholar 

  376. Wettstein, F. O., Noll, H., Penman, S.: Effect of cycloheximide on ribosomal aggregates engaged in protein synthesis in vitro. Biochim. biophys. Acta (Amst.) 87, 525 (1964).

    CAS  Google Scholar 

  377. White, J. P., Cantor, C. R.: Role of magnesium in the binding of tetracycline to Escherichia coli ribosomes. J. molec. Biol. 58, 397 (1971).

    Article  PubMed  CAS  Google Scholar 

  378. Wiley, P. F., Mackellar, F. A.:The structure of sparsomycin. J. Amer. chem. Soc. 92, 417 (1970).

    Article  CAS  Google Scholar 

  379. Wilhelm, J. M., Corcoran, J. W.: Antibiotic glycosides. VI. Definition of the 50 S ribosomal subunit of Bacillus subtilis 168 as a major determinant of sensitivity to erythromycin A. Biochemistry 6, 2578 (1967).

    Article  PubMed  CAS  Google Scholar 

  380. Wittmann, H. G., Stoeffler, G., Hindennach, I., Kurland, C. G., Randall-Hazalbauer, L., Birge, E. A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R. R., Bickle, T. A.: Correlation of 30 S ribosomal proteins of E. coli isolated in different laboratories. Molec. gen. Genet. 111, 327 (1971).

    Article  PubMed  CAS  Google Scholar 

  381. Wolfe, A. D., Hahn, F. E.: Erythromycin mode of action. Science 143, 1445 (1964).

    Article  PubMed  CAS  Google Scholar 

  382. Wolfe, A. D., Hahn, F. E.: Mode of action of chloramphenicol. IX. Effects of chloramphenicol upon a ribosomal amino acid polymerization system and its binding to bacterial ribosome. Biochim. biophys. Acta (Amst.) 95, 146 (1965).

    CAS  Google Scholar 

  383. Yamaguchi, H., Tanaka, N.: Site of action of mikamycins A and B in polypeptide synthesizing systems. J. Biochem. 61, 18 (1967).

    PubMed  CAS  Google Scholar 

  384. Zimmermann, R. A., Garvin, R. T., Gorini, L.: Alteration of a 30 S ribosomal protein accompanying the ram mutation in E. coli. Proc. nat. Acad. Sci. (Wash.) 68, 2263 (1971).

    Article  CAS  Google Scholar 

  385. Zimmermann, R. A., Rosset, R., Gorini, L.: The nature of phenotypic masking exhibited by drug dependent str A mutants of E. coli. Pers. communication (1972).

    Google Scholar 

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Authors
  1. Akira Kaji
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F. E. Hahn

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© 1973 Springer-Verlag Berlin · Heidelberg

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Kaji, A. (1973). Mechanism of Protein Synthesis and Use of Inhibitors in the Study of Protein Synthesis. In: Hahn, F.E. (eds) Progress in Molecular and Subcellular Biology 3. Progress in Molecular and Subcellular Biology, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65578-4_3

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  • DOI: https://doi.org/10.1007/978-3-642-65578-4_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65580-7

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