Skip to main content

Abstract

The high energy electron microscope has been used mainly in the field of material science and of minerology. Especially in minerology, the advantage of the higher penetrating power of electrons at higher accelarating voltage which enables the observation of material in bulk is enormous and the high voltage device becomes indispensable.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Cosslett, V. E.: High voltage electron microscopy and its application in biology. Phil. Trans. B. 261, 35–44 (1971a).

    Article  Google Scholar 

  • Cosslett, V. E.: The scope and limitations of high voltage electron microscopy. J. Ultrastruct. Res. 37, 255–257 (1971b).

    Google Scholar 

  • Dupouy, G.: Electron Microscopy at Very High Voltages,Advances in Optical and Electron Microscopy, Vol. 2, 168–250 (1968). Academic Press. Ed.: Barer and Cosslett.

    Google Scholar 

  • Dupouy, G., Perrier, F., Durriru, L.: Microscopie électronique. L’ observation de la matière vivante au moyen d’un microscope électronique fonctionnant sous très haute tension. C. R. Acad. Se. 251, 2836–2841 (1960).

    CAS  Google Scholar 

  • Dupouy, G., Perrier, F.: Principaux résultats obtenus aves un microscope électronnique fonctionnant sous un million de volts. Fifth International Congress for Electron Microscopy, Philadelphia, Breese Jr. S. S., ed. 1, A-3 (1962).

    Google Scholar 

  • Fujita, H., Kawasaki, Y., Fujibayashi, E., Kajiwara, S., Taoka, T.: Metallurgical investigation with 500 Kv electron microscope, Japan. J. appi. Physics 6, 214–230 (1967).

    Article  CAS  Google Scholar 

  • Glaeser, R. M., Budinger, T. F., Aebersold, P. M., Thomas, G. T.: Radiation damage in biological specimens. Microscopie électronique Vol. 1, 463–464 (1970).

    Google Scholar 

  • Hama, K., Nagata, F.: High resolution observation of biological section with a high voltage electron microscope. J. Electron Microscopy 19, 170–175 (1970a).

    CAS  Google Scholar 

  • Hama, K., Nagata, F.: A stereoscopic observation of tracheal epithelium of mouse by means of the high voltage electron microscope. J. Cell Biol. 45, 654–659 (1970b).

    Article  PubMed  CAS  Google Scholar 

  • Hama, K., Porter, K. R.: An application of high voltage electron microscopy to the study of biological materials. J. de Microscopie 8, 149–158 (1969).

    Google Scholar 

  • Hashimoto, H.: Energy dependence of extinction distance and transmissive power for electron waves in crystals. J. appi. Physics 35, 277–290 (1964).

    Article  Google Scholar 

  • Huxley, H. E.: The double array of filaments in cross striated muscle. J. biophys. bio- chem. Cytol. 3, 631–647 (1957).

    Article  CAS  Google Scholar 

  • Kobayashi, K.: Fundamentals of HVEM for organic materials, reported at U.S.A.-Japan Seminar on “New trends in high voltage electron microscopy” (1971a). Personal Communication.

    Google Scholar 

  • Kobayashi, K.: On the electron microscopy of colloidal particles in liquids, Ibd. (1971 b). Personal Communication.

    Google Scholar 

  • Kobayashi, K., Sakaoka, K.: The changes of polymer crystals due to irradiation with electrons accelerated at various voltages. Bull. Institute Chem. Res. (Kyoto Univer-sity) 42, 473–493 (1964).

    CAS  Google Scholar 

  • Luft, J. H.: Improvements in epoxy resin embedding methods. J. Cell Biol. 9, 409–414 (1961).

    Article  CAS  Google Scholar 

  • Nagata, F., Fukai, K.: Effects of electron irradiation on growth of spore, reported at U.S.A.-Japan Seminar on “New trends in high voltage electron microscopy” (1971). Personal Communication, N Agata, F., Hama, K.: Observation of biological specimens with a high voltage electron microscope. 27th Annual Proceedings EMSA, (1970).

    Google Scholar 

  • Nagata, F., Hama, K.: Observation of biological specimens with a high voltage electron miscroscope. 27th Annual Proceeding EMSA, (1970).

    Google Scholar 

  • Nagata, F., Hama, K.: Chromatic aberration on electron microscope image of biological sectioned specimen. J. Electron Microscopy 20, 6–10 (1971).

    Google Scholar 

  • Novikoff, P. M., Novikoff, A. B., Quintana, N., Hauw, J. J.: Golgi apparatus, gerì, and lysosomes of neurons in rat dorsal root ganglia, studied by thick section and thin section cytochemistry. J. Cell Biol. 50, 859–886 (1971).

    Article  PubMed  CAS  Google Scholar 

  • PARSONS, D. F.: Beam efficiency, plasmon inelestic scatter and radiation damage in the high voltage microscope, J. appi. Physics 43, (1972) in press.

    Google Scholar 

  • Rice, R. V., Moses, J., Wray, G.: High voltage electron microscopy of unstained frozen striated muscle, reported at U.S.A.-Japan Seminar on “New trends in high voltage electron Microscopy” (1971). Personal Communication.

    Google Scholar 

  • Ris, H.: Use of the high voltage electron microscope for the study of thick biological specimens. J. de Microscopie 8, 761–766 (1969).

    Google Scholar 

  • Szirmae, A., Fesher, R. M., Moses, J., Rice, R. V.: Contrast and resolution of thick sections of muscle in the HVEM, reported at U.S.A.-Japan Seminar on “New trends in high voltage electron Microscopy” (1971). Personal Communication.

    Google Scholar 

  • Ueda, R., Nonoyama, M.: The observation of thick specimens by high voltage electron microscopy. Experiment with molybdenite films at 50–500 Kv. Japan. J. appi. Physics 6, 557–566 (1967).

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1973 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Hama, K. (1973). High Voltage Electron Microscopy. In: Koehler, J.K. (eds) Advanced Techniques in Biological Electron Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65492-3_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-65492-3_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65494-7

  • Online ISBN: 978-3-642-65492-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics