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Phagenanreicherung und Ultrazentrifugation

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Bakterien-, Phagen- und Molekulargenetik

Part of the book series: Praktikum der Genetik ((550,volume 1))

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Zusammenfassung

Für viele Versuche der Mikroben- und Molekulargenetik werden Bakterien und Phagen in großer Menge und hochgereinigt benötigt. Die Anzucht von Bakterien bereitet im allgemeinen keine methodischen Schwierigkeiten, vorausgesetzt Kulturgefäße großen Fassungsvermögens und eine leistungsstarke Zentrifuge sind vorhanden. Die Anzucht, Konzentration und Reinigung von Phagen dagegen ist methodisch aufwendiger. Am Beispiel des Phagen T4, der hier stellvertretend für viele andere Phagen steht, soll im folgenden in 3 Versuchen seine Anzucht, Anreicherung und Reinigung gezeigt werden.

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Literatur

  • Bowen, T. J.: An Introduction to Ultracentrifugation. London: Wiley Interscience 1971

    Google Scholar 

  • Cantoni, G. L. and D. R. Davies (Eds.): Procedures in Nucleic Acid Research. London: Harper and Row 1967

    Google Scholar 

  • Dirkx, S. J.: Analytical Density Gradient Centrifugation. Broschüre der Beckman Instruments GmbH München

    Google Scholar 

  • Gros, F. and H. Matthaei (Eds.): Practical Molecular Genetics. Cell-free Protein Synthesis. Berlin: Springer Verlag. In Vorbereitung

    Google Scholar 

  • Habel, K. and N. P. Salzman (Eds.): Fundamental Techniques in Virology. London: Academic Press 1969

    Google Scholar 

  • Maramorosch, K. and H. Koprowski (Eds.): Methods in Virology, 4 Bände. London: Academic Press 1967 — 1968

    Google Scholar 

  • Norris, J. R. and D. W. Ribbons (Eds.): Methods in Microbiology, bisher 6 Bände. London: Academic Press 1969 — 1972

    Google Scholar 

  • Perry, E. S. and C. J. van Oss (Eds.): Progress in Separation and Purification. London: Wiley-Interscience 1969

    Google Scholar 

  • Schachman, H. K.: Ultracentrifugation in Biochemistry. London: Academic Press 1959

    Google Scholar 

  • Adams, M. H.: Bacteriophages. London: Interscience Publ. Inc. 1959 (insb. 454–460)

    Google Scholar 

  • Stent, G. S.: Molecular Biology of Bacterial Viruses. London: Freeman & Comp. 1963

    Google Scholar 

  • Eisenstark, A.: Bacteriophage Techniques. In: Methods in Virology 1, 450–524, London: Academic Press 1967.

    Google Scholar 

  • Sargeant, K.: Large-Scale Bacteriophage Production. In: Advances in Applied Microbiology 13, 121–137 (1970)

    Article  Google Scholar 

  • Thomas, C. A., Jr. and J. Abelson: The Isolation and Characterisation of DNA from Bacteriophage. In: Procedures in Nucleic Acid Research (Eds. G. L. Cantoni and D. R. Davies). London: Harper & Row, 553–555 (1966)

    Google Scholar 

  • Albertsson, P.-Å.: Two-Phase Separation of Viruses. In: Methods in Virology 2, 303–321 (1967)

    Google Scholar 

  • Albertsson, P.-Å.: Partition of Cell Particles and Macromolecules in Polymer Two-Phase Systems. In: Advances in Protein Chemistry 24. 309–341 (1970)

    Article  PubMed  CAS  Google Scholar 

  • Albertsson, P.-Å.: Partition in Polymer Two-Phase Systems — some Recent Results. In: Progress in Separation and Purification 2, 105–120. London: Wiley 1969

    Google Scholar 

  • Anderson, N. G. and G. B. Cline: New Centrifugal Methods for Virus Isolation. In: Methods in Virology 1, 137–178. London: Academic Press 1967

    Google Scholar 

  • Eichenberger, W.: Trennung biologischer Partikel durch Zentrifugation im Dichtegradienten. Chimia 23, 85–94 (1969

    CAS  Google Scholar 

  • Vinograd, J. and J. E. Hearst: Equilibrium Sedimentation of Macromolecules and Viruses in a Density Gradient. In: Fortschritte der Chemie organischer Naturstoffe 20, 372–422 (1962)

    CAS  Google Scholar 

  • Brakke, M. K.: Density-Gradient Centrifugation. In: Methods in Virology 1, 93–118. London: Academic Press 1967

    Google Scholar 

  • Kellenberger, G., M. L. Zichichi and J. Weigle: A Mutation Affecting the DNA Content of Bacteriophage Lambda and its Lysogenizing Properties. J. Biol. Chem. 3, 399–408 (1961)

    CAS  Google Scholar 

  • Szybalski, W.: Use of Cesium Sulfate for Equilibrium Density Gradient Centrifugation. In: Nucleic Acids, Part 2, 330–360. London Academic Press 1968

    Google Scholar 

  • Weigle, J. M. Meselsohn and K. Paigen: Density Alterations Associated with Transducing Ability in the Bacteriophage Lambda. J. Mol. Biol. 1, 379–386 (1959)

    Article  CAS  Google Scholar 

  • Mahler, H. R. and E. H. Cordes: Biological Chemistry. New York, London: Harper & Row 1969, S. 41 ff, 143 ff

    Google Scholar 

  • Sober, H. A. (Editor): Handbook of Biochemistry. Selected Data for Molecular Biology. Cleveland: The Chemical Rubber Co. 1968, S. C10, H4. (S-Werte)

    Google Scholar 

  • Sund, H. und K. Weber: Untersuchungen liber milchzuckerspaltende Enzyme, XIII Größe und Gestalt der ß-Galactosidase aus E. coli. Biochem. Zeitschr. 337, 24–34 (1963)

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Lehrstuhl Biologie der Mikroorganismen, Ruhr-Universität Bochum, Deutschland

    o. Professor Dr. phil. Ulrich Winkler

  2. Department of Internal Medicine, Yale University, New Haven, Connecticut, USA

    Dr. rer. nat. Wilfried Wackernagel (Wissenschaftlicher Assistent an demselben Lehrstuhl z. Z.)

Authors
  1. o. Professor Dr. phil. Ulrich Winkler
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  2. Privatdozent Dr. phil. Wolfgang Rüger
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  3. Dr. rer. nat. Wilfried Wackernagel
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© 1972 Springer-Verlag Berlin · Heidelberg

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Winkler, U., Rüger, W., Wackernagel, W. (1972). Phagenanreicherung und Ultrazentrifugation. In: Bakterien-, Phagen- und Molekulargenetik. Praktikum der Genetik, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65455-8_5

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  • DOI: https://doi.org/10.1007/978-3-642-65455-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-05988-2

  • Online ISBN: 978-3-642-65455-8

  • eBook Packages: Springer Book Archive

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