Further Stuides of Hormone-Regulated Senescence in Rumex Leaf Tissue

  • Jonathan Goldthwaite
Conference paper


Senescence of leaf tissue and attached leaves can be inhibited by all of the three major groups of higher plant growth hormones. Leaf senescence can also be promoted by ethylene and abscisic acid. Which hormones are active in a given case seems to be primarily determined by the species used, although some evidence indicates this may be an oversimplification (e.g. Aspinall, et al., 1967; Goldthwaite and Laetsch, 1968). Previous work (Whyte and Luckwill, 1966; Goldthwaite and Laetsch, 1968) showed that net breakdown of chlorophyll and protein in excised leaf tissue of Rumex spp., is strongly inhibited by very low concentrations of gibberellic acid (GA3). Also described were several aspects of the behavior of the Rumex system including time courses of chlorophyll and protein loss, reversibility of the GA3 effect and activity of the cytokinin 6-benzylaminopurine (BAP). This report describes experiments carried out to further our understanding of hormone-controlled senescence in this plant. Included are studies of the effect of GA3 and BAP on senescence induced in attached leaves and the effect of other growth-regulators and combined applications of GA3 and cytokinins to excised leaf discs.


Leaf Disc Gibberellic Acid Leaf Senescence Plant Growth Substance Attached Leaf 
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  1. ASPINALL, D., L.G. PALEG and F.T. ADDICOTT (1967). Abscisin II and some hormone-regulated plant responses. Aust. J. Biol. Sci. 20, 869–882.Google Scholar
  2. BACK, A. and A.E. RICHMOND (1969). An interaction between the effects of kinetin and gibberellin in retarding leaf senescence. Physiol. Plant. 22, 1207–16.PubMedCrossRefGoogle Scholar
  3. BACK, A. and A.E. RICHMOND (1971). Interrelations between gibberellic acid, cytokinins and abscisic acid in retarding leaf senescence. Physiol. Plant. 24, 76–9.CrossRefGoogle Scholar
  4. BEEVERS, L. (1968). Growth regulator control of senescence in leaf discs of nasturtium (Tropaeoium majus), in “Biochemistry and Physiology of Plant Growth Substances” (Eds. F. Wightman and G. Setterfield). The Runge Press Ltd., Ottawa. 1960.Google Scholar
  5. CHIN, T. and L. BEEVERS (1970). Changes in endogenous growth regulators in nasturtium leaves during senescence. Planta 92, 178–88.CrossRefGoogle Scholar
  6. EL-ANTABLY, H.M.M., P.F. WAREING and J. HILLMAN (1967). Some physiological responses to D, L-Abscisin (Dormin). Planta 73, 74–90.CrossRefGoogle Scholar
  7. FLETCHER, R.A., T. OEGEMA, and R.F. HORTON (1969). Endogenous gibberellin levels and senescence in Taraxacum officinale. Planta 86, 98–102.CrossRefGoogle Scholar
  8. FLETCHER, R.A. and D.J. OSBORNE (1966). A simple bioassay for gibberellic acid. Nature 211 743–4.PubMedCrossRefGoogle Scholar
  9. GOLDTHWAITE, J.J. (1968). “Experimental investigations of leaf tissue senescence”. Thesis, University of California, Berkeley.Google Scholar
  10. GOLDTHWAITE, J.J. and L. BOGORAD (1971). A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase. Anal. Biochem. 41, 57–66.PubMedCrossRefGoogle Scholar
  11. GOLDTHWAITE, J.J. and W.M. LAETSCH (1967). Regulation of senescence in bean leaf discs by light and chemical growth regulators. Plant Physiol. 42, 1757–62.PubMedCrossRefGoogle Scholar
  12. GOLDTHWAITE, J.J. and W.M. LAETSCH (1968). Control of senescence in Rumex leaf discs by gibberellic acid. Plant Physiol. 43, 1855–8.PubMedCrossRefGoogle Scholar
  13. HARDWICK, K., M. WOOD, and H.W. WOOLHOUSE (1968). Photosynthesis and respiration in relation to leaf age in Perilla frutescens. (L.) Britt. New Riytol. 67, 79–86.CrossRefGoogle Scholar
  14. KIRK, J.T.O. and R.A.E. TILNEY-BASSETT (1967). “The Plastids”. W.H. Freeman and Company, London.Google Scholar
  15. REYNOLDS, T. (1969). Senescence retardation in dock leaves by soluble peptone. Nature 223, 505–6.CrossRefGoogle Scholar
  16. WHYTE, P. and L.C. LUCKWILL (1966). A sensitive bioassay for gibberellins based on retardation of leaf senescence in Rumex obtusifolius. Nature 210, 1360.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1972

Authors and Affiliations

  • Jonathan Goldthwaite
    • 1
  1. 1.The Biological LaboratoriesHarvard UniversityCambridgeUSA

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