Isozymes of Cellulase in the Abscission Zone of Phaseolus vulgaris
Cellulase activity in the abscission zone of Red Kidney bean was previously shown to be partially soluble in a low salt buffer and partially soluble in a high salt buffer (Lewis and Varner, 1970). This suggested the presence of more than one molecular form of the enzyme. The work reported here was done to elucidate the multiple nature of cellulase in the abscission zone.
KeywordsCellulase Activity Abscission Zone Plant Growth Substance High Salt Buffer High Salt Extract
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- FRANKEL-CONRAT,H.(1957). Methods for investigating the essential groups for enzyme activity, in “Methods in Enzymology” (Ed. S.P. COLOWICK and N.O. KAPLAN) vol. IV. P. 268. Academic Press, New York. 1957.Google Scholar
- HAGLUND, H. (1967). Isoelectric focusing in natural pH gradients-A technique of growing importance for fractionation and characterization of proteins. Science Tools 14, 17–23.Google Scholar
- NELSEN, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153, 375.Google Scholar