Morphological and Cultural Characteristics of Culiseta inornata and Aedes vexans Mosquito Cell Lines

  • Janiece S. McHale
  • B. H. Sweet
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 55)

Abstract

The two mosquito cell lines discussed in this paper were initiated on hemolymph-free medium, one from pupal tissue of Aedes vexans and another from newly emerged adult tissue of Culiseta inornata (Sweet and Dupree, 1968; Sweet and McHale, 1970). Grace’s medium (Grand Island Biological Co.) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics was used routinely. Growth from explants was not observed for a period of 1–3 months, after which small round cells appeared free-floating in the medium. The number of cells increased rapidly, and a culture-split ratio of 1: 10 or 1: 15 has been carried out weekly for two years for both cell lines. Optimum growth in both cell lines occurs at 28°C, followed by limited growth at 32°C and 20°C and cell death occurs at 37°C. Cells can be stored in the frozen state at -70°C and -120°C with 10% glycerol added to the medium.

Keywords

Glycerol Expense Eucalyptus Sera Hemocyte 

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Copyright information

© Springer-Verlag, Berlin · Heidelberg 1971

Authors and Affiliations

  • Janiece S. McHale
  • B. H. Sweet

There are no affiliations available

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