Morphological and Cultural Characteristics of Culiseta inornata and Aedes vexans Mosquito Cell Lines
The two mosquito cell lines discussed in this paper were initiated on hemolymph-free medium, one from pupal tissue of Aedes vexans and another from newly emerged adult tissue of Culiseta inornata (Sweet and Dupree, 1968; Sweet and McHale, 1970). Grace’s medium (Grand Island Biological Co.) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics was used routinely. Growth from explants was not observed for a period of 1–3 months, after which small round cells appeared free-floating in the medium. The number of cells increased rapidly, and a culture-split ratio of 1: 10 or 1: 15 has been carried out weekly for two years for both cell lines. Optimum growth in both cell lines occurs at 28°C, followed by limited growth at 32°C and 20°C and cell death occurs at 37°C. Cells can be stored in the frozen state at -70°C and -120°C with 10% glycerol added to the medium.
KeywordsInsect Cell Line Small Round Cell Walter Reed Army Institute Antigenic Relationship Grand Island Biological
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