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Gas Chromatography-Mass Spectrometry

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Concepts in Biochemical Pharmacology

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Abstract

In choosing a method by which to identify drug metabolites, the quantity of material which is available is a prime consideration. In this regard, pharmacologists find themselves midway between natural products chemists and biochemists. Usually the former has reasonably large quantities of material available in a relatively high state of purity. The biochemist on the other hand, is usually forced to deal with submicrogram quantities; radioactivity may be the only objective evidence of the existence of a compound. This is a consequence of the biochemist’s use of purified enzyme preparations which only allow for the conversion of mg quantities under the carefully controlled conditions he usually wishes to maintain. Attempts to scale up such processes may bring about a whole new set of problems. The pharmacologist interested in drug metabolism deals with enzyme systems as present in intact organs or at least parts of organs (liver slices, etc.), and in general this permits conversion of somewhat larger quantities. In fact, most drugs in use today are employed at mg rather than μg levels and so the investigation of their metabolism is at least practicable. This chapter discusses the advantages and disadvantages of mass spectrometry, especially as combined with gas chromatography, for such investigations.

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Authors
  1. A. M. Guarino
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  2. H. M. Fales
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Editors and Affiliations

  1. Department of Health, Education and Welfare, National Institutes of Health, Bethesda, MD, 20014, USA

    Bernard B. Brodie  & James R. Gillette  & 

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Guarino, A.M., Fales, H.M. (1971). Gas Chromatography-Mass Spectrometry. In: Brodie, B.B., Gillette, J.R., Ackerman, H.S. (eds) Concepts in Biochemical Pharmacology. Handbook of Experimental Pharmacology / Handbuch der experimentellen Pharmakologie, vol 28 / 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65177-9_12

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