Abstract
The last decade has seen a considerable amount of research effort directed towards the indentification and characterization of the components required for a separate genetic apparatus in large cellular organelles (see Roodyn and Wilkie, 1967). As a consequence of these studies we now know that mitochondria and chloroplasts possess their own DNA as well as an entire and separate transcriptional and translational machinery (S. Nass, 1969). The information content of mitochondrial DNA is, however, not sufficient to code for all of the enzymes, RNA species and regulatory factors that are required for the biosynthesis of all the macromolecules of the organelle, much less to code for all factors necessary for mitochondrial function. It has, therefore, been the aim of our own research program to define the extent and nature of the genetic message contained in and expressed by the mitochondria of baker’s yeast (Saccharomyces cerevisiae).
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References
Allen, N. E., Macquillan, A. M.: Target analysis of mitochondrial genetic units in yeast. J. Bact. 97, 1142 (1969).
Atwood, K. C, Norman, A.: On the interpretation of multi-hit survival curves. Proc. nat. Acad. Sci. ((Wash.) 35, 696 (1949).
Bauer, W., Vinograd, J.: The interaction of closed circular DNA with intercalative dyes I The superhelix density of SV40 DNA in the presence and absence of dye. J. molec. Biol. 33, 141 (1968).
Billheimer, F. E., Avers, C. J.: Nuclear and mitochondrial DNA from wild-type and petite yeast: circularity, length, and buoyant density. Proc. nat. Acad. Sci. (Wash.) 64, 739 (1969).
Borst, P.: Mitochondrial DNA: structure, information content, replication and transcription. Soc. exp. Biol., Symp. 24, (1969) (in press).
Clark-Walker, G. D., Linnane, A. W.: The biogenesis of mitochondria in Saccharomyces cerevisiae. A comparison between cytoplasmic respiratory-deficient mutant yeast and chloramphenicol-inhibited wild type cells. J. Cell Biol. 34, 1 (1967).
Elliott, W. H.: The effects of antimicrobial agents on deoxyribonucleic acid polymerase. Biochem. J. 86, 562 (1963).
Ephrussi, B., Hottinguer, H., Chimenes, A.: Action de Pacriflavine sur les levures. I. La mutation «petite colonie». Annales de lTnstitut Pasteur 76, 351 (1949).
Ephrussi, B., Slonimski, P. P., Yotsuyanagi, Y., Tavlitzki, J.: Variations physiologiques et cyto- logiques de la levure au cours de cycle de la croissance aerobie. C. R. Lab.Carlsberg 26, 87 (1956).
Fukuhara, H.: Relative proportions of mitochondrial and nuclear DNA in yeast under various conditions of growth. Europ. J. Biochem. 11, 135 (1969).
Fukuhara, H., Faures, M., Genin, C.: Comparison of RNA’s transcribed in vivo from mitochondrial DNA of cytoplasmic and chromosomal respiratory deficient mutants. Molec. gen. Genet. 104, 264 (1969).
Grossman, L. S., Goldring, E. S., Marmur, J.: Preferential synthesis of yeast mitochondrial DNA in the absence of protein synthesis. J. molec. Biol. 46, 367 (1969).
Hendler, R. W.: Procedure for simultaneous assay of two β-emitting isotopes with the liquid scintillation counting technique. Anal. Biochem. 7, 110 (1964).
Hollenberg, C. P., Borst, P., Thuring, R. W. J., Van Bruggen, E. F. J.: Size, structure, and genetic complexity of yeast mitochondrial DNA. Biochim. biophys. Acta (Amst.) 186, 417 (1969).
Huang, M., Biggs, D. R., Clark-Walker, G. D., Linnane, A. W.: Chloramphenicol inhibition of the formation of particulate mitochondrial enzymes of Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 114, 434 (1966).
Kellerman, G. M., Biggs, D. R., Linnane, A. W.: Biogenesis of mitochondria. XI. A comparison of the effects of growth-limiting oxygen tension, intercalating agents, and antibiotics on the obligate aerobe, Candida parapsilosis. J. Cell Biol. 42, 378 (1969).
Kraml, J. R., Mahler, H. R.: Biochemical correlates of respiratory deficiency —VIII. A precipitating antiserum against cytochrome oxidase of yeast and its use in the study of respiratory deficiency. Immunochemistry 4, 213 (1967).
Leon, S. A., Mahler, H. R.: Isolation and properties of mitochondrial RNA from yeast. Arch. Biochem. 126, 305 (1968).
Lepecq, J. B., Paoletti, C.: A fluorescent complex between ethidium bromide and nucleic acids. J. molec. Biol. 20, 87 (1967).
Mackler, B., Douglas, H. C., Will, S., Hawthorne, D.C., Mahler, H. R.: Biochemical correlates of respiratory deficiency. IV. Composition and properties of respiratory particles from mutant yeasts. Biochemistry 4, 2016 (1965).
Mahler, H. R., Mackler, B., Slonimski, P. P., Grandchamp, S.: Biochemical correlates of respiratory deficiency II. Antigenic properties of respiratory particles. Biochemistry 3, 677 (1964).
Mehrotra, B. D., Mahler, H. R.: Characterization of some unusual DNAs from the mitochondria from certain “petite” strains of Saccharomyces cerevisiae. Arch. Biochem. 128, 685 (1968).
Meyer, R. R., Simpson, M. V.: DNA biosynthesis in mitochondria: differential inhibition of mitochondrial and nuclear DNA polymerase by the mutagenic dyes ethidium bromide and acriflavin. Biochem. biophys. Res. Commun. 34, 238 (1969).
Moustacchi, E., Williamson, D. H.: Physiological variations in satellite components of yeast DNA detected by density gradient centrifugation. Biochem. biophys. Res. Commun. 23, 56 (1966).
Nagai, S.: Production of respiration-deficient mutants of yeast by some quinone-imine dyes. Exp. Cell Res. 27, 14 (1962).
Nagai, S., Yanagishima, N., Nagai, H.: Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bact. Rev. 25, 404 (1961).
Nass, M. M. K.: Mitochondrial DNA: advances, problems, and goals, studies of size and structure of mitochondrial DNA related to biogenesis and function of the organelle. Science 165, 25 (1969).
Nass, S.: The significance of the structural and functional similarities of bacteria and mito-chondria. Int. Rev. Cytol. 55, 55 (1969).
Ogur, M., St. John, R., Nagai, S.: Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science 125, 98 (1957).
Perlman, P. S., Mahler, H. R.: (1) Intracellular localization of enzymes in yeast. Arch. Biochem. 136, 245 (1970).
Perlman, P. S., Mahler, H. R.: (2) Formation of yeast mitochondria III: biochemical properties of mitochondria isolated from a cytoplasmic petite mutant of yeast. J. Bioenerget. 1, 113 (1970).
Rabinowitz, M.: Extranuclear DNA. Bull. Soc. Chim. biol. (Paris) 50, 311 (1968).
Rabinowitz, M. Getz, B. S., Casey, J., Swift, H.: Synthesis of mitochondrial and nuclear DNA in anaerobically grown yeast during the development of mitochondrial function in response to oxygen. J. molec. Biol. 41, 381 (1969).
Radloff, R., Bauer, W., Vinograd, J.: A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc. nat. Acad. Sci. (Wash.) 57, 1514 (1967).
Reilly, C., Sherman, F.: Glucose repression of cytochrome a synthesis in cytochrome- deficient mutants of yeast. Biochim. biophys. Acta (Amst.) 95, 640 (1965).
Roodyn, D. B., Wilkie, D.: The biogenesis of mitochondria. London: Methuen 1968.
Sarachek, A.: The induction by Mn++ of heritable respiratory deficiency in non-dividing populations of Saccharomyces. Biochim. biophys. Acta (Amst.) 33, 227 (1959).
Schatz, G., Saltzgaber, J.: Protein synthesis by yeast promitochondria in vivo. Biochem. biophys. Res. Commun. 67, 996 (1969).
Sherman, F., Slonimski, P. P.: Respiration-deficient mutants of yeast II. Biochemistry. Biochim. biophys. Acta (Amst.) 90, 1 (1964).
Slonimski, P. P.: Recherches sur le formation des enzymes respiratoires chez le levure. Paris: Masson 1953.
Slonimski, P. P., Ephrussi, B.: Action de Tacriflavine sur les levures V. Le systeme des cytochromes des mutants «petite colonie». Ann. Inst. Pasteur 77, 47 (1949).
Slonimski, P. P., Perrodin, G., Croft, J. H.: Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal “petites”. Biochem. biophys. Res. Commun. 30, 232 (1968).
South, D. J., Mahler, H. R.: RNA synthesis in yeast mitochondria: a derepressible activity. Nature (Lond.) 218, 1226 (1968).
Waring, M. J.: Complex formation between ethidium bromide and nucleic acids. J. molec. Biol. 13, 269 (1965).
Yotsuyanagi, Y.: Etudes sur le chondriome de la levure II. Chondriomes des mutants a deficience respiratoire. J. Ultrastruct. Res. 7, 141 (1962).
Zylber, E., Vesco, C., Penman, S.: Selective inhibition of the synthesis of mitochondria- associated RNA by ethidium bromide. J. molec. Biol. 44, 195 (1969).
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Mahler, H.R., Mehrotra, B.D., Perlman, P.S. (1971). Formation of Yeast Mitochondria. V. Ethidium Bromide as a Probe for the Functions of Mitochondrial DNA. In: Hahn, F.E. (eds) Proceedings of the Research Symposium on Complexes of Biologically Active Substances with Nucleic Acids and Their Modes of Action. Progress in Molecular and Subcellular Biology, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65141-0_22
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