Skip to main content
SpringerLink
Log in

Resorption von Eisen and chemisch verwandten Metallen in vitro and in vivo; die Spezifität eines eisenbindenden Systems in der Mucosa des Jejunums von Ratten

Absorption of Iron and Chemically Related Metals in vitro and in vivo; the Specificity of an Iron-binding System in the Jejunal Mucosa of Rats

  • Conference paper
Frontiers of Nuclear Medicine/Aktuelle Nuklearmedizin

  • 36 Accesses

Summary

Iron-deficient rats absorb from tied jejunal loops in vivo not only more iron than normal rats but also the chemically related metals cobalt, manganese and even zinc, but not copper. The retention of these metals, the absorption of which is increased, is equal in both normal and anemic animals.

Everted sacs in vitro, prepared from jejunal segments of iron-deficient rats, transfer iron, the chemically related metals already mentioned, and in addition nickel, to a greater extent than those of normal rats; under the experimental conditions in vitro this is not the case for copper. The uptake of these metals by the mucosal tissue was also greater with segments of iron-deficient rats than with those of normal ones.

Finally, mutual inhibition could be shown during absorption of iron and cobalt as well as of iron and manganese. The latter experiments were done in situ on tied loops of jejunum of rats in vivo.

These results can be explained by the assumption of an iron-binding system in the mucosa responsible for absorption: this system shows the highest degree of specificity for iron, followed by cobalt, nickel, manganese and zinc, but apparently does not react with copper.

As acceptors for iron and the chemically related metals, the proteins of the cell membrane may play a role comparable with that of the reticulocyte membrane responsible for the uptake and incorporation of iron in the reticulocytes. The coincidence of these processes leads us to believe that the mucosal epithelium and reticulocytes possess similar mechanisms which are responsible for uptake and/or transcellular transport of iron.

Die englische Fassung dieses Beitrages erschien in: Trace Element Metabolism in Animals (C. F. Mills, Ed.). Edinburgh-London: E. & S. Livingstone 1970. — Abdruck in deutscher Sprache erfolgt mit freundlicher Erlaubnis des Verlages E. & S. Livingstone.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Allen, D. W., Jandl, I. H.: Kinetics of intracellular iron in rabbit reticulocytes. Blood 15, 71 (1960).

    PubMed  CAS  Google Scholar 

  2. Bannerman, R. M., O’Brien, I. R. P., Wilts, L. J.: Studies on iron metabolism IV. Iron absorption in experimental iron deficiency. Blood 20, 532 (1962).

    CAS  Google Scholar 

  3. Blanc, B.: Etude isotopique des protéines impliquées dans la résorption et dans le métabolisme du fer. Vortrag gehalten auf der 3. Jahrestagung der Ges. f. Nuclearmedizin in Lausanne, 21.–23. X. 1965.

    Google Scholar 

  4. Blanc, B. Issliker, H.: Repartition du fer daps diverses fractions tissulaires de la muqueuse intestinale au cours de 1’absorption. Mise en évidence et caractérisation partielle d’une mucoproteine fixatrice du fer. Helv. physiol. acta 23, 145 (1965).

    CAS  Google Scholar 

  5. Blanc, B.: Rôle des protéines impliquées dans la résorption et le métabolisme du fer. In: Radioisotope in der Gastroenterologie (Vannotti u. Hoffmann, Hgb.). Stuttgart: F. K. Schattauer 1967, S. 113.

    Google Scholar 

  6. Bothwell, Th. H.: Iron overload in the Bantu. In: Iron metabolism ( F. Gross, Ed.). Berlin-Göttingen-Heidelberg: Springer 1964, p. 362.

    Google Scholar 

  7. Bothwell, Th. H., Pirzio-Biroli, G., Finch, C.: Iron absorption I. Factors influencing absorption. J. Lab. Clin. Med. 51, 24 (1958).

    PubMed  CAS  Google Scholar 

  8. Brown, E. B., Rother, M. L.: Studies of the mechanism of iron absorption I. I. on uptake by the normal rat. J. Lab. Clin. Med. 62, 357 (1963).

    PubMed  CAS  Google Scholar 

  9. Brown, E. B., Rother, M. L.: Studies of the mechanism of iron absorption II. Influence of iron deficiency and other conditions of iron uptake by rats. J. Lab. Clin. Med. 62, 804 (1963).

    PubMed  CAS  Google Scholar 

  10. Charley, P. J., Stitt, C., Shore, E., Saltman, P.: Studies in the regulation of intestinal iron absorption. J. Lab. Clin. Med. 61, 397 (1963).

    CAS  Google Scholar 

  11. Charlton, R. W., Lacobs, P., Torrance, J. D., Bothwell, T. H.: The role of intestinal mucosa in iron absorption. J. Clin. Invest. 44, 543 (1965).

    Article  PubMed  CAS  Google Scholar 

  12. Chirasiri, L., Izak, G.: The effect of acute haemorrhage and acute haemolysis on the intestinal iron absorption in the rat. Brit. J. Haemat. 12, 611 (1966).

    Article  PubMed  CAS  Google Scholar 

  13. Conrad, M. E., Crosby, W. H.: Intestinal mucosal mechanisms controlling iron absorption. Blood 22, 406 (1963).

    PubMed  CAS  Google Scholar 

  14. Deobald, H. J., Elvehjem, C. A.: The effect of feeding high amounts of soluble iron and aluminium salts. Amer. J. Physiol. 111, 118 (1935).

    CAS  Google Scholar 

  15. Deuel: Zit. nach: Biochemisches Taschenbuch (H. M. Rauen, Hgb.). Berlin-Göttingen-Heidelberg: Springer 1958, S. 791.

    Google Scholar 

  16. Dowdle, E. B., Schachter, D., Schenker, H.: Active transport of Fe59 by everted segments of rat duodenum. Amer. J. Physiol. 198, 609 (1960).

    PubMed  CAS  Google Scholar 

  17. Duthie, H. L.: The relative importance of the duodenum in the intestinal absorption of iron. Brit. J. Haemat. 10, 59 (1964).

    Article  PubMed  CAS  Google Scholar 

  18. Finch, C. A., Loden, B.: Body iron exchange in man. J. Clin. Invest. 38, 392 (1959).

    Article  PubMed  CAS  Google Scholar 

  19. Forth, W.: In: Radioisotope in der Hämatologie. I. Intern. Symp. Freiburg 1962 (W. Keiderling, G. Hoffmann, Hgb.). Stuttgart: F. K. Schattauer 1963, S. 61.

    Google Scholar 

  20. Forth, W.: Untersuchungen über die Resorption von Eisen and chemisch verwandten Schwermetallen an Därmen normaler and anämischer Ratten in vivo und in vitro; ein Beitrag zur Frage der Spezifität des Eisenbindenden Systems in der Mucosa. Habil.-Schrift Homburg/Saar 1966.

    Google Scholar 

  21. Forth, W., Andres, H.: Methode zur Erzeugung eines Eisenmangels bei Ratten. Arzneimittelforschung 19, 363 (1969).

    PubMed  CAS  Google Scholar 

  22. Forth, W., Leopold, G., Rummel, W.: Eisendurchtritt von der Mucosa- zur Serosaseite und umgekehrt an isolierten eisenarmen and normalen Segmenten von Jejunum and Ileum. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 261, 434 (1968).

    Article  CAS  Google Scholar 

  23. Forth, W., Pfleger, K., Rummel, W.: Zur Wirkung von Komplexbildnern auf die Fe-Resorption der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 245, 89 (1963).

    Google Scholar 

  24. Forth, W., Rummel, W.: Eisenresorption an isolierten Dünndarmpräparaten von normalen and anämischen Ratten. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 252, 205 (1965).

    CAS  Google Scholar 

  25. Forth, W., Rummel, W.: Beziehungen zwischen Eisen-Konzentration and -Resorption am isolierten Dünndarm normaler and anämischer Ratten. Med. Pharmacol. exp. 14, 289 (1966).

    CAS  Google Scholar 

  26. Forth, W., Rummel, W.: Abhängigkeit der Eisenresorption von der Eisenbindung durch den Darm. Med. Pharmacol. exp. 14, 384 (1966).

    CAS  Google Scholar 

  27. Forth, W., Rummel, W.: Zur Frage der Regulation der Eisenresorption durch Gastrof errin, ein eisenbindendes Protein des Magensaftes. Klin. Wschr. 46, 1003 (1968).

    Article  PubMed  CAS  Google Scholar 

  28. Forth, W., Rummel, W. Becker, P. J.: Die vergleichende Prüfung von Bindung and Durchtritt von Eisen, Kobalt and Kupfer durch isolierte Jejunumsegmente normaler and anamischer Ratten. Med. Pharmacol. exp. 15, 15, 179 (1966).

    CAS  Google Scholar 

  29. Forth, W., Rummel, W., Crüsemann, D., Pfleger, K.: Die Bedeutung der Bindung in der Zellmembran für die Fe-Aufnahme der Reticulozyten. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 257, 275 (1967).

    Article  Google Scholar 

  30. Forth, W., Rummel, W., Crüsemann, D., Simon, J.: Die Bindung von Eisen and verwandten Schwermetallen in der Membran and ihre Aufnahme in Reticulozyten. In I. Intern. Sympos. caber Stoffwechsel and Permeabilitat von Erythrozyten and Thrombozyten ( Deutsch, Gerlach, Moser, Eds.). Stuttgart: G. Thieme 1968.

    Google Scholar 

  31. Forth, W., Rummel, W. Pfleger, K.: Der Einf luß von Liganden auf die Retention von Eisen nach oraler Verabfolgung an normalen and anamischen Ratten. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 261, 225 (1968).

    Article  CAS  Google Scholar 

  32. Forth, W., Rummel, W. Seifen, E.: Der Einf luß verschiedener Liganden auf Eisendurchtritt and -bindung an isolierten Därmprdparaten normaler and anämischer Ratten. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 252, 224 (1965).

    CAS  Google Scholar 

  33. Forth, W., Pfleger, K., Rummel, W., Seifen, E., Richmond, Sp. J.: Der Elnfluß verschiedener Liganden auf Resorption, Verteilung and Ausscheidung von Eisen nach oraler Verabfolgung. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 252, 242 (1965).

    Google Scholar 

  34. Forth, W., Seifen, E.: Der Einfluß von Komplexbildnern auf die 59Fe-Resorption am isolierten Darm der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 241, 556 (1961).

    Article  CAS  Google Scholar 

  35. Granick, S.: Iron metabolism and hemochromatosis. Bull. N. Y. Acad. Med. 25, 403 (1949).

    PubMed  CAS  Google Scholar 

  36. Granick, S.: Structure and physiological functions of ferritin. Physiol. Rev. 31, 489 (1951).

    PubMed  CAS  Google Scholar 

  37. Hahn, P. F., Bale, W. F., Lawrence, E. O., Whipple, G. H.: Radioactive iron and its metabolism in anemia. J. exp. Med. 69, 739 (1939).

    Article  PubMed  CAS  Google Scholar 

  38. Hahn, P. F., Bale, W. F., Ross, J. F., Balfour, W. M., Whipple, G. H.: Radioactive iron absorption by gastrointestinal tract. J. exp. Med. 78, 169 (1943).

    Article  PubMed  CAS  Google Scholar 

  39. Hahn, P. F., Carothers, E. L., Darby, W. I., Martin, M., Sheppard, C. W., Cannon, R. 0., Beam, A. S., Densen, P. M., Petersen, J. C., McClellan, G. S.: Iron metabolism in human pregnancy as studied with the radioactive isotope 59Fe. Amer. J. Obst. Gyn. 61, 477 (1951).

    CAS  Google Scholar 

  40. Hallberg, L., Sölvell, L.: Iron absorption studies. Acta med. Scand. 168 (Suppl. 358), 71 (1960).

    Google Scholar 

  41. Helbock, H. J., Saltman, P.: The transport of iron by rat intestine. Biochem. Biophys. Acta 135, 979 (1967).

    Article  PubMed  CAS  Google Scholar 

  42. Heller, M. D., Kern, F.: Absorption of lactic acid from an isolated intestinal segment in the intact rat. Proc. Soc. Exp. Biol. Med. 127, 1103 (1968).

    CAS  Google Scholar 

  43. Hevesy, G. v., Lockner, D.: Iron metabolism in health and in the neoplastic state. Arkiv f ör Kemi 19, Nr. 26, 303 (1961).

    Google Scholar 

  44. Iida, H., Moore, E. W., Broitman, S. A., Zamcheck, N.: Effect of pH on active transport of D-glucose in the small intestine of hamsters. Proc. Soc. exp. biol. med. 127, 730 (1968).

    PubMed  CAS  Google Scholar 

  45. Jandl, J. H., Inman, I. K., Simmons, R. L., Allen, D. W.: Transfer of iron from serum iron-binding protein to human reticulocytes. J. clip. Invest. 28, 161 (1959).

    Article  Google Scholar 

  46. Jandl, J. H., Katz, J. H.: The plasma-to-cell cycle of transferrin. J. clip. Invest. 42, 314 (1963).

    Article  CAS  Google Scholar 

  47. Katz, J. H., Jandl, J. H.: The role of transferrin in the transport of iron into the developing red cell. In: Iron metabolism ( F. Gross, Ed.). Berlin- Göttingen-Heidelberg: Springer 1964, p. 103.

    Google Scholar 

  48. Kaufman, R. M., Pollack, S., Crosby, W. H.: Iron-deficient diet: effect in rats and humans. Blood 28, 726 (1966).

    PubMed  CAS  Google Scholar 

  49. Krantz, S., Goldwasser, E., Jacobson, L. O.: Studies on erythropoiesis XIV. The relationship of humoral stimulation to iron absorption. Blood 14, 654 (1959).

    PubMed  CAS  Google Scholar 

  50. Lafferty, F. W., Spencer, G. E., Pearson, O. E.: Effects of androgens, estrogens and high calcium intakes on bone formation and resorption in osteoporosis. Amer. J. Med. 36, 514 (1964).

    Article  PubMed  CAS  Google Scholar 

  51. Laurell, C. B.: Studies on the transportation and metabolism of iron in the body with special reference to the iron binding component in human plasma. Acta physiol. Scand. Suppl. 46, 14 (1947).

    Google Scholar 

  52. Laurell, C. B.: Iron transportation. In: Iron in clinical medicine (R. O. Wallerstein, S. R. Mettier, Eds.). Berkeley-Los Angeles: Univ. of California Press 1958, p. 8.

    Google Scholar 

  53. Lynch, S. R., Berelowitz, I., Seftel, H. C., Miller, G. B., Krawitz, P., Charlton, R. W., Bothwell, T. H.: Osteoporosis in Johannesburg bantu males, its relationship to siderosis and ascorbic acid deficiency. Amer. J. clin. Nutr. 20, 799 (1967).

    PubMed  CAS  Google Scholar 

  54. Manis, J. G., Schachter, D.: Active transport of iron by intestine: features of the two-step mechanism. Amer. J. Physiol. 203, 73 (1962).

    CAS  Google Scholar 

  55. Manis, J. G., Schachter, D.: Active transport of iron by intestine: effects of oral iron and pregnancy. Amer. J. Physiol. 203, 81 (1962).

    PubMed  CAS  Google Scholar 

  56. McCance, R. A., Widdowson, E. M.: Absorption and excretion of iron. Lancet 2, 680 (1937).

    Article  Google Scholar 

  57. McGowan, J. P., Crichton, A.: Iron deficiency in pigs. Biochem. J. 18, 265 (1928).

    Google Scholar 

  58. Mendel, G. S., Weller, R. I., Mangalik, A.: Studies on iron absorption II. The absorption of iron in experimental anemias of diverse etiology. Blood 22, 450 (1963).

    PubMed  CAS  Google Scholar 

  59. Morgan, E. H., Laurell, C. B.: Studies on the exchange of iron between transferrin and reticulocytes. Brit. J. Haemat. 9, 471 (1963).

    Article  PubMed  CAS  Google Scholar 

  60. Phillips, C. S. G., Williams. R. J. P.: Inorganic chemistry I and II. Oxford: Clarendon 1965.

    Google Scholar 

  61. Pollack, S., George, J. N., Reba, R. C., Kaufmann, R. M., Crosby, W. H.: The absorption of nonferrous metals in iron deficiency. J. clin. Invest. 44, 1470 (1965).

    Article  PubMed  CAS  Google Scholar 

  62. Reimann, F., Fritsch: Zit. nach E. Starkenstein: Eisen. In: Handbuch der exp. Pharmakologie. 3. Band, 2. Teil (A. Heffter, W. Heubner, Hgb.). Berlin: Springer 1934, S. 859.

    Google Scholar 

  63. Roe, J. H., Dwyer, H. M.: Proc. Soc. Exp. Biol. Med. 41, 603 (1939). Zit. nach: The rat (E. J. Farris, J. Q. Griffith, Eds.). Philadelphia-London-Montreal: Lippincott 1949, p. 168.

    Google Scholar 

  64. Ruliffson, W. S., Hopping, J. M.: Maturation, iron deficiency and ligands in enteric radioiron transport in vitro. Amer. J. Physiol. 204, 171 (1963).

    PubMed  CAS  Google Scholar 

  65. Schade, A. L., Oyama, S., Reinhart, R. W., Miller, J. R.: Bound iron and unsaturated iron-binding capacity of serum: rapid and reliable determination. Proc. Soc. Exp. Biol. Med. 87, 443 (1954).

    PubMed  CAS  Google Scholar 

  66. Smith, N. J., Schulz, J.: In: Iron in clinical medicine (R. O. Wallerstein, S. R. Mettier, Eds.). Berkeley-Los Angeles: Univ. of California Press 1958, p. 65.

    Google Scholar 

  67. Sturgeon, P.: Studies on iron requirements in infants and children I. Normal values for serum iron, copper and free erythrocyte protoporphyrin. Pediatrics 13, 107 (1954).

    PubMed  CAS  Google Scholar 

  68. Valberg, L. S., Olatunbosun, D.: Alteration in cobalt absorption in patients with disorders of iron metabolism. Gastroenterology 54, 1279 (1968).

    Google Scholar 

  69. Wesson: Zit. nach: Biochemisches Taschenbuch (H. M. Raven, Hgb.). Berlin- Göttingen-Heidelberg: Springer 1958, S. 793.

    Google Scholar 

  70. Wilson, T. H., Wiseman, G.: The use of sacs of everted small intestine for the study of the transferrence of substances from the mucosal to the serosal surface. J. Physiol. (London) 123, 116 (1954).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Pharmakologie, Homburg, Saar, Germany

    W. Forth

  2. Toxikologie der Universität des Saarlandes, Homburg, Saar, Germany

    W. Forth

Authors
  1. W. Forth
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Kantonsspital, CH-8006, Zurich, Switzerland

    Wolfgang Horst (Direktor der Universitätsklinik und Poliklinik für Nuklearmedizin und Radiotherapie) (Direktor der Universitätsklinik und Poliklinik für Nuklearmedizin und Radiotherapie)

Rights and permissions

Reprints and permissions

Copyright information

© 1971 Springer-Verlag, Berlin · Heidelberg

About this paper

Cite this paper

Forth, W. (1971). Resorption von Eisen and chemisch verwandten Metallen in vitro and in vivo; die Spezifität eines eisenbindenden Systems in der Mucosa des Jejunums von Ratten. In: Horst, W. (eds) Frontiers of Nuclear Medicine/Aktuelle Nuklearmedizin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65092-5_9

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-65092-5_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-65094-9

  • Online ISBN: 978-3-642-65092-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation