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Genetic Stability in Haploid Cell Cultures

  • A. Ziauddin
  • K. J. Kasha
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 12)

Abstract

Karyotype instability is an inherent problem of in vitro cultures, since most cultures consist of a heterogeneous mass of cells in different states of differentiation and dedifferentiation. Haploid cultures, in spite of possible initiation from a uniform population of uni-nucleate haploid microspores or haploid protoplasts, exhibit similar types of instability. Because of their origin from single haploid cells they can provide a unique opportunity for studying the factors leading to variability. Cell cultures are also of importance for advances in somatic fusion and gene transfer into defined plant cells, since in vitro cultures and subsequent regeneration of plants are usually necessary. However, induced variability might interfere with directed and controlled genetic manipulation (Lorz and Brown 1986).

Keywords

Callus Culture Anther Culture Genetic Stability Microspore Culture Haploid Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bajaj YPS (1983) Haploid protoplasts. In: Giles KL (ed) Plant protoplasts. Int Rev Cytol Suppl16. Academic Press, New York London, pp 113 - 141Google Scholar
  2. Bajaj YPS (1984) The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theor Appl Genet 67:525 - 528Google Scholar
  3. Bajaj YPS (1986) In vitro regeneration of diverse plants and the cryopreservation of germplasm in wheat (Triticum aestivum L.). Cereal Res Commun 14 (3): 305 - 311Google Scholar
  4. Bajaj YPS, Gosch G, Ottma M, Weber A, Gr5bler A (1978) Production of polyploid and aneuploid plants from anthers and mesophyll protoplasts of Atropa belladonna and Nicotiana tabacum. Indian J Exp BioI 16: 947 - 953Google Scholar
  5. Bayliss MW (1980) Chromosomal variation in plant tissues in culture. In: Vasil K (ed) Int Rev Cytol Suppl IIA. Academic Press, New York London, pp 113 - 143Google Scholar
  6. Bennici A, Buiatti M, D'Amato F (1968) Nuclear conditions in haploid Pelargonium in vivo and in vitro. Chromosoma 24: 194 - 201CrossRefGoogle Scholar
  7. Bennici A, Buiatti M, D'Amato F, Pagliaj M (1971) Nuclear behaviour in Haplopappus gracilis grown in vitro on different culture media. Les cultures de tissues de plantes. Coli Int CNRS 193:245 - 250Google Scholar
  8. Binding H, Nehls R, Scheider 0, Sopory SK, Wenzel G (1978) Regeneration of mesophyll protoplasts isolated from dihaploid clones of Solanum tuberosum. Physiol Plant 43:52- 56Google Scholar
  9. Bourgin JP, Missonier C, Chupeau Y (1976) Culture of mesophyll protoplasts from haploid and diploid Nicotiana sylvestris. CR Acad Sci D282: 1853 - 1856Google Scholar
  10. Brown JS, Wernsman EA, Schnell RJ (1983) Effect of a second cycle of anther culture on flure-cured lines of tobacco. Crop Sci 23: 729 - 733CrossRefGoogle Scholar
  11. Chaleff RS (1981) Genetics of higher plants - application of cell culture. Cambridge Univ Press, New York, pp 184Google Scholar
  12. Chen CC, Chen CM (1980) Changes in chromosome number in microspore callus of rice during successive subcultures. Can J Genet Cytol 22:607 -614Google Scholar
  13. Chu CC, Wang YX, Sang JL (1987) Long term plant regeneration from callus cultures of haploid wheat. Plant Cell Tissue Org Cult 11:221 - 226Google Scholar
  14. Chu Q, Zhang Z, Gao Y (1985) Cytogenetical analysis on aneuploids obtained from pollenclones of rice (Oryza sativa L.). Theor App Genet 71: 506 - 512Google Scholar
  15. Clapham D (1973) Haploid Hordeum plants form anthers in vitro. Z Pflanzenziicht 69: 142 - 155Google Scholar
  16. Collins GB, Legg PD, Kasperbauer MJ (1972) Chromosome number in anther-derived haploids of two Nicotiana species. J Hered 63:113 -118Google Scholar
  17. D'Amato F (1977) Cytogenetics of differentiation in tissue and cell cultures. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berl in HeidelbergGoogle Scholar
  18. New York, pp 343 - 357Google Scholar
  19. D'Amato F (1978) Chromosome number in cultured cells and regenerated plants. In: Thorpe A (ed) Frontiers of plant tissue culture 1978. Int Assoc Plant Tissue Cult, Calgary, pp 287 - 295Google Scholar
  20. D'Amato F (1985) Cytogenetics of plant cells and tissue cultures and their regenerates. CRC Crit Rev Plant Sci 3:73 -112Google Scholar
  21. D'Amato F, Devreux M, Scarasci GT (1965) Haploids. Caryologia 18: 377Google Scholar
  22. D'Amato F, Bennici A, Cionini PG, Baroncelli S, Lupi MC (1980) Nuclear fragmentation followed by mitosis as mechanism for wide chromosome number variation in tissue cultures, its implications for plant regeneration. In: Sala F, Parisi B, Cella R, Ciferri O (eds) Plant cell cultures: results and perspectives. Elsevier North Holland, Amsterdam, pp 67 - 72Google Scholar
  23. Dang Ha D, Pernes J (1982) Androgenesis in pearl millet. 1. Analysis of plants obtained from microspore culture. Z Pflanzenphysiol 108:317 - 327Google Scholar
  24. Day A, Ellis THN (1984) Chloroplast DNA deletions associated with wheat plants regenerated from pollen. Possible basis for maternal inheritance of chloroplasts. Cell 30: 359-368Google Scholar
  25. Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9: 671 - 678CrossRefGoogle Scholar
  26. De Paepe R, Prat D, Huget T (1982) Heritable nuclear DNA changes in doubled haploid plants obtained by pollen culture of Nicotiana sylvestris. Plant Sci Lett 18: 11 - 28Google Scholar
  27. De Paepe R, Prat D, Huget T (1983) Effects of consecutive androgenesis on morphology and fertility in Nicotiana sylvestris. Can J Bot 61: 2038 - 2046CrossRefGoogle Scholar
  28. Dhillon SS, Wernsman EA, Miksche JP (1983) Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (Nicotiana tabacum). Can J Genet Cytol 25: 169 - 173Google Scholar
  29. Dix PJ, Street HE (1974) Effects of p-fluorophenylalanine on the growth of cell lines differing in ploidy and derived from Nicotiana sylvestris. Plant Sci Lett 3: 283 - 288CrossRefGoogle Scholar
  30. Dolezel J, Novak FJ (1984) Effect of plant tissue culture media on the frequency of somatic mutations in Tradescantia stamen hairs. Pflanzenphysiol 144: 51 - 58Google Scholar
  31. Engvild KL, Linde-Laursen I, Lundquist A (1972) Anther cultures of Datura innoxia: flower bud stage and embryoid level of ploidy. Hereditas 72:331- 332Google Scholar
  32. Evans DA (1989) Somaclonal variation - genetic basis and breeding applications. TAG 5 (2): 46 - 50Google Scholar
  33. Evans DA, Gamborg OL (1982) Chromosome stability of cell suspension cultures of Nicotiana spp. Plant Cell Rep 1: 104 - 107CrossRefGoogle Scholar
  34. Furner LJ, King J, Gamborg OL (1978) Plant regeneration from protoplasts isolated from a predominantly haploid suspension culture of Datura innoxia. Plant Sci Lett 11: 169 - 176CrossRefGoogle Scholar
  35. Gegenbach BG, Connelly JA, Pring DR, Conde MF (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59: 161 - 167CrossRefGoogle Scholar
  36. George L, Rao PS (1982) In vitro induction of pollen embryos and plantlets in Brassica juncea through anther culture. Plant Sci Lett 26: 111 - 116CrossRefGoogle Scholar
  37. Gupta N, Carlson PS (1972) Preferential growth of haploid plant cells in vitro. Nature New BioI 239: 86Google Scholar
  38. Hahne G, Lorz H (1987) Cryopreservation of embryogenic callus cultures from barley (Hordeum vulgare). Z Pflanzenz(lcht 99: 330 - 332Google Scholar
  39. Hoffman F, Thomas E, Wenzel G (1982) Anther culture as breeding tool in rape. II. Progeny analysis of androgenetic lines and induced mutants from haploid cultures. Theor Appl Genet 61: 225-232Google Scholar
  40. Hu H (1986) Variability and gamete expression in pollen-derived plants in wheat. In: Hu H, Yang H (eds) Haploids of higher plants in vitro. China Acad Publ, Beijing; and Springer Berlin Heidelberg New York, pp 67 -78Google Scholar
  41. Hu H, Huang B (1987) Application of pollen-derived plants to crop improvement. Int Rev Cytol 107:293 - 313Google Scholar
  42. Huang B (1986) Ultrastructural aspects of pollen embryogenesis in Hordeum, Triticum and Paeonia. Int Rev CytoI107: 91 - 117Google Scholar
  43. Jacobsen E (1981) Polyploidization in leaf callus tissue and regenerated plants of dihaploid potato. Plant Cell Tissue Org Cult 1:77 - 84Google Scholar
  44. Johnson SS, Phillips RL, Rines HW (1987) Possible role of heterochromatin in chromosome breakage induced by tissue culture in oats (Avena sativa L.). Genome 29: 439 - 446CrossRefGoogle Scholar
  45. Kameya T, Hinata K (1970) Induction of haploid plants from pollen grains of Brassica. Jpn J Breed 20: 82Google Scholar
  46. Karp A, Bright SWJ (1985) On the causes and origins of somaclonal variation. Oxford Surv Plant Mol Cell BioI 2: 199 - 234Google Scholar
  47. Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast derived potato plants. Theor Appl Genet 63: 265 - 272CrossRefGoogle Scholar
  48. Kasha KJ (1974) Haploids from somatic cells. In: Kasha KJ (ed) Haploids in higher plants: advances and potential. Univ Press, Guelph, pp 67 - 87Google Scholar
  49. Kasha KJ, Kott L, Seguin-Swartz G (1982) Genetic stability of haploid cell cultures. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Thkyo, pp 405 - 408Google Scholar
  50. Kasperbauer MJ, Collins GB (1972) Reconstitution of diploids from leaf tissue of anther-derived haploids in tobacco. Crop Sci 12: 98 - 101CrossRefGoogle Scholar
  51. Kasperbauer MJ, Buchner RC, Springer WD (1980) Haploid plants by anther-panicle culture of tall fescue. Crop Sci 20:103 -107Google Scholar
  52. Keller WA, Armstrong KC (1977) Embryogenesis and plant regeneration in Brassica napus anther cultures. Can J Bot 55:1383 -1388Google Scholar
  53. Keller WA, Armstrong KC (1979) Stimulation of embryogenesis and haploid production by elevated temperature treatments. Theor Appl Genet 55: 65 - 67CrossRefGoogle Scholar
  54. Kemble RJ, Bedbrook JR (1980) Low molecular weight circular and linear DNA molecules in mitochondria from normal and male-sterile cytoplasms of Zea mays. Nature (London) 284: 565 - 566CrossRefGoogle Scholar
  55. Kibler B, Neumann KH (1980) On cytogenetic stability of cultured tissue and cell suspensions of haploid and diploid origin. In: Sala F, Parisi B, Cella R, Ciferri D (eds) Plant cell cultures: results and perspectives. Elsevier, Amsterdam, pp 59 - 66Google Scholar
  56. Kott LS, Flack S, Kasha KJ (1986) A comparative study of initiation and development of embryogenic callus from haploid embryos of several barley cultivars. II. Cytophotometry of embryos and callus. Can J Bot 64: 2107-2112Google Scholar
  57. Kudirka Uf, Schaeffer GW, Baenzinger PS (1983) Cytogenetic characteristic of wheat plants regenerated from anther culture of 'Centurk'. Can J Genet Cytol 25: 513 - 517Google Scholar
  58. Larkin PJ (1987) Somac1onal variation: history, method and meaning. Iowa State J Res 61(4):393 -434Google Scholar
  59. Lee M, Phillips RL (1987) Genomic rearrangements in maize induced by tissue culture. Genome 29: 122 - 128CrossRefGoogle Scholar
  60. Liang G, Xu A, Hoang-Thng (1987) Direct generation of wheat haploids via anther culture. Crop Sci 27: 336 - 339Google Scholar
  61. Lorz H, Brown PfH (1986) Variability in tissue culture derived plants - possible origin: advantages and drawbacks. In: Horn W, Jensen CJ, Odenbach W, Schieder 0 (eds) Genetic manipulation in plant breeding. De Gruyter, Berlin New York, pp 513 - 534Google Scholar
  62. Matthews PS, Vasil IK (1975) The dynamics of cell proliferation in haploid and diploid tissues of Nicotiana tabacum. Z Pflanzenphysiol 77: 222 - 236Google Scholar
  63. McComb JA, McComb AJ (1977) The cytology of plantlets derived from cultured anthers of Nicotiana sylvestris. New Phytol 79: 679 - 688CrossRefGoogle Scholar
  64. McCoy TJ, Phillips RL, Rines HW (1982) Cytogenetic analysis of plants regenerated from oat (Avena sativa L.) tissue cultures: High frequency of partial chromosome loss. Can J Genet Cyto124:37 - 50Google Scholar
  65. McNay JW, Chourey PS, Pring DR (1984) Molecular analysis of genomic stability of mitochondrial DNA in tissue cultured cells of maize. Theor Appl Genet 67: 433 - 437CrossRefGoogle Scholar
  66. Mercy ST, Zapata FJ (1986) Chromosomal behaviour of anther culture derived plants of rice. Plant Cell Rep 5: 215 - 218CrossRefGoogle Scholar
  67. Mix G, Wilson HM, Foroughi-Wehr B (1978) The cytological status of plants of Hordeum vulgare L. regenerated from microspore callus. Z Pflanzenziicht 80: 89 - 99Google Scholar
  68. Niizeki M, Kita F (1980) Studies on plant and cell tissue culture. X. Diploid plant production by callus culture of haploid Nicotiana species. J Fac Agric Hokkaido Univ 60: 1 - 9Google Scholar
  69. Novak FJ, Vyskot B (1975) Karyology of callus cultures derived from Nicotiana tabacum haploids and ploidy of regenerants. Z Pflanzenzilcht 75: 62 - 70Google Scholar
  70. Ohyama K, Nitsch JP (1972) Flowering haploid plants obtained from protoplasts of tobacco leaves. Plant Cell Physiol 13: 229 - 236Google Scholar
  71. Ono H, Larter EN (1976) Anther culture of triticale. Crop Sci 16: 120 - 122CrossRefGoogle Scholar
  72. Oono K (1981) In vitro methods applied to rice. In: Thorpe TA (ed) Plant tissue culture. Academic Press, New York London, pp 273 - 298Google Scholar
  73. Oono K (1984) Regulation of somatic mutation in rice tissue culture. Int Symp Genetic manipulation in crops (Abstr), Beijing, China, p 5Google Scholar
  74. Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeat is lost. Cell 29:537 - 550Google Scholar
  75. Partanen CR (1963) Plant tissue culture in relation to developmental cytology. Int Rev Cytol 15: 215 - 243PubMedCrossRefGoogle Scholar
  76. Ramulu KS, Dijkhuis P, Roest S (1989) Patterns of phenotypic and chromosome variation in plants derived from protoplast cultures of monohaploid, dihaploid and diploid genotypes and in somatic hybrids of potato. Plant Sci 60: 101 - 110CrossRefGoogle Scholar
  77. Rines HW (1983) Oatanther culture: genotype effects on callus initiation and the production of a haploid plant. Crop Sci 23: 268 - 272CrossRefGoogle Scholar
  78. Saalbach G, Koblitz H (1977) Karyological instabilities in callus cultures from haploid barley plants. Biochem Physiol Pflanzen 171: 469 - 473Google Scholar
  79. Sacristan MD (1971) Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris. Chromosoma 33:273 - 283Google Scholar
  80. Schaeffer GW (1982) Recovery of heritable variability in anther-derived doubled-haploid rice. Crop Sci 22: 1160 - 1164CrossRefGoogle Scholar
  81. Scowcroft WR, Larkin PJ (1982) Somac1onal variation: a new option for plant improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York London, pp 159 - 178Google Scholar
  82. Seguin-Swartz G, Kott L, Kasha KJ (1984) Development of haploid cell lines from immature barley (Hordeum vulgare) embryos. Plant Cell Rep 3: 95 - 97CrossRefGoogle Scholar
  83. Snape JW, Sitch LA, Simpson E, Parker BB (1988) Thsts for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the Hordeum bulbosum system. Theor Appl Genet 75: 509 - 513CrossRefGoogle Scholar
  84. Sopory SK, Thn BH (1979) Regeneration and cytological studies of anther and pollen calli of dihaploid Solanum tuberosum. Z Pflanzenziicht 82:31 - 35Google Scholar
  85. Sunderland N (1977) Nuclear cytology. In: Street HE (ed) Plant tissue and cell culture, 2nd edn. Univ Press, Berkeley, Cal, pp 177 - 205Google Scholar
  86. Sunderland N, Collins GB, Dunwell JM (1974) The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill. Planta 117: 227 - 241CrossRefGoogle Scholar
  87. Thomas E, Wenzel G (1975) Embryogenesis from rnicrospores of Brassica napus. Z Pflanzenziicht 74: 77 - 81Google Scholar
  88. Thomas E, Hoffman F, Wenzel G (1975) Haploid plants from microspores of rye. Z Pflanzenziicht 75: 106 - 113Google Scholar
  89. Thomas E, Hoffman F, Potrykus I, Wenzel G (1976) Protoplast regeneration and stem embryogenesis of haploid androgenetic rape. Mol Gen Genet 145: 245 - 248CrossRefGoogle Scholar
  90. Thomas E, Bright SWJ, Franklin J, Lancaster V, Miflin BJ, Gibson R (1982) Variation amongst protoplast- derived potato plants (Solanum tuberosum cv. Maris Bard ). Theor Appl Genet 62: 65-68Google Scholar
  91. Ting YC (1985) Meiosis and fertility of anther culture-derived maize plants. Maydica 30: 161 - 169Google Scholar
  92. Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73: 16 - 19CrossRefGoogle Scholar
  93. Thay HS, Miao SH, Widholm JM (1986) Factors affecting haploid plant regeneration from maize anther culture. J Plant Physiol 126: 33 - 40CrossRefGoogle Scholar
  94. Wang YY, Sun CS, Wang CC, Chien NF (1973) The induction of pollen plantlets of 'friticale and Capsicum annum from anther culture. Sci Sin 16: 147Google Scholar
  95. Wilson HM, Mix G, Foroughi-Wehr B (1978) Early rnicrospore divisions and subsequent formation of rnicrospore calluses at high frequency in anthers of Hordeum vulgare L. J Exp Bot 29:227 - 238Google Scholar
  96. Withers LA (1983) Germplasm preservation through tissue culture: an overview. Proc Worksh Cell and tissue culture techniques for cereal crop improvement. Inst Genet Acad Sin and IRRI, Manila, PhilippinesGoogle Scholar
  97. Ziauddin A, Kasha KJ (1990) Long-term callus cultures of diploid barley (Hordeum vulgare) I I. Effect of auxins on chromosomal status of cultures and regeneration of plants. Euphytica (accepted)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. Ziauddin
  • K. J. Kasha
    • 1
  1. 1.Department of Crop ScienceUniversity of GuelphGuelphCanada

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