Advertisement

Wild Tomato (Solanum carolinense L.): Anther Culture and the Induction of Haploids

  • T. L. Reynolds
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 12)

Abstract

Solanum carolinense L. (wild tomato), a member of the Solanaceae, is a herbaceous perennial of southeastern Canada and the central and eastern United States (Britton and Brown 1970). The plant reproduces vegetatively and sexually (Ilnicki et al. 1962), is armed with prickles, and has been designated one of the ten most troublesome pasture weeds in the southeastern United States (Smith and Calvert 1980). S. carolinense contains the alkaloids solanine and solanidine and has been used pharmacologically as a sedative and antispasmodic (Culbreth 1927). Extracts from the fruits have been shown to have antibacterial and antifungal properties, although the active constituents of the extracts are not known (Pitts et al. 1969). This plant has been used in my laboratory to study various aspects of regeneration and differentiation from androgenic and somatic tissues in culture (Reynolds 1984 a, 1986 a, b, c, 1987 a, b, 1989 a, b). This chapter summarizes the work on anther culture and the regulation of androgenesis in this system.

Keywords

Anther Culture Callus Induction Medium Wild Tomato Bicellular Pollen Induction Haploid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anand VV, Arekal GD (1979) In vitro culture of excised anthers of Solanum mammosum L. Indian J Exp Bioi 17: 444 - 446Google Scholar
  2. Britton N, Brown A (1970) An illustrated flora of the northern United States and Canada, vol 3, 2nd edn. Dover, New YorkGoogle Scholar
  3. Cappadocia M, Cheng DSK, Ludlum R (1984) Plant regeneration from in vitro culture of anthers of Solanum chacoense Bitt. and interspecific diploid hybrids S. tuberosum L.xS. chacoense Bitt. Theor Appl Genet 69: 139 - 143Google Scholar
  4. Culbreth DMR (1927) Materia medica and pharmacology, 7th edn. Lea & Febiger, PhiladelphiaGoogle Scholar
  5. Dunwell JM (1979) Anther culture of Nicotiana tabacum: the role of the culture vessel atmosphere in pollen embryo induction and growth. J Exp Bot 30: 419 - 428CrossRefGoogle Scholar
  6. Dunwell JM, Sunderland N (1973) Anther culture of Solanum tuberosum L. Euphytica 22: 317 - 323CrossRefGoogle Scholar
  7. Harn C (1972) Production of plants from anthers of Solanum nigrum cultured in vitro. Caryologia 25: 429 - 437Google Scholar
  8. Hasenstein KH, Evans ML (1986) Calcium ion dependency of ethylene production in segments of primary roots of Zea mays. Physiol Plant 67: 570 - 575PubMedCrossRefGoogle Scholar
  9. Hepler PK (1985) Calcium restriction prolongs metaphase in dividing Tradescantia stamen hair cells. J Cell Bioi 100: 1363 -1368Google Scholar
  10. Horner M, Street HE (1978) Pollen dimorphism-origin and significance in pollen plant formation by anther culture. Ann Bot (London) 42:763 - 771Google Scholar
  11. Ilnicki RD, Tisdell TF, Fertig SN, Furrer AH (1962) Life history studies as related to weed control in the northeast. 3. Horse nettle. Bull 368 Rhode Island Agric Exp Stn, Kingston, Rhode IslandGoogle Scholar
  12. Irikura Y (1975) Induction of haploid plants by anther culture of tuber-bearing species and interspecific hybrids of Solanum. Potato Res 18: 133 - 140CrossRefGoogle Scholar
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473 - 497CrossRefGoogle Scholar
  14. Pitts OM, Thompson HS, Hoch JH (1969) Antibacterial activity of Solanum carolinense L. J Pharm Sci 58: 379 - 380PubMedCrossRefGoogle Scholar
  15. Poovaiah BW, Reddy ASN (1987) Calcium messenger system in plants. CRC Crit Rev Plant Sci 6: 47 - 103PubMedCrossRefGoogle Scholar
  16. Raghavan V (1979) Embryogenic determination and ribonucleic acid synthesis in pollen grains of Hyoscyamus niger. Am J Bot 66:36- 39Google Scholar
  17. Raghavan V (1986) Embryogenesis in angiosperms. Cambridge Univ Press, LondonGoogle Scholar
  18. Raina SK, Iyer RD (1973) Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena L. Z Pflanzenzticht 70: 275 - 280Google Scholar
  19. Rashid A, Siddiqui AW, Reinert J (1981) Ultrastructure of embryogenic pollen of Nicotiana tabacum var. Badischer Burley. Protoplasma 107: 375-385Google Scholar
  20. Reynolds TL (1984a) Callus formation and organogenesis in anther cultures of Solanum carolinense L. J Plant PhysioI117: 157 - 161Google Scholar
  21. Reynolds TL (1984b) An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during normal ontogeny and induced embryogenic development. Am J Bot 71: 490 - 504CrossRefGoogle Scholar
  22. Reynolds TL (1985) Ultrastructure of anomalous pollen development in embryogenic anther cultures of Hyoscyamus niger. Am J Bot 72: 44 - 51CrossRefGoogle Scholar
  23. Reynolds TL (1986a) Pollen embryogenesis in anther cultures of Solanum carolinense L. Plant Cell Rep 5: 273 - 275CrossRefGoogle Scholar
  24. Reynolds TL (1986b) Somatic embryogenesis and organogenesis from callus cultures of Solanum carolinense. Am J Bot 73: 914 - 918CrossRefGoogle Scholar
  25. Reynolds TL (1986c) Effects of chlorsulfuron, valine and isoleucine, on division and tracheary element differentiation in cell suspension cultures of Solanum carolinense L. J Plant Physiol 125: 179 - 184CrossRefGoogle Scholar
  26. Reynolds TL (1987 a) A possible role for ethylene during IAA-induced pollen embryogenesis in anther cultures of Solanum carolinense L. Am J Bot 74:967-969Google Scholar
  27. Reynolds TL (1987b) Cytodifferentiation in cell suspension cultures of Solanum carolinense: Effects of 2,4-D and kinetin. JPlant Physiol 130: 373 - 383CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • T. L. Reynolds
    • 1
  1. 1.Department of BiologyUniversity of North CarolinaCharlotteUSA

Personalised recommendations