Alfalfa (Medicago spp.): In Vitro Production of Haploids

  • N. Zagorska
  • R. Stereva
  • P. Robeva
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 12)


Alfalfa is among the most important fodder crops. It is used either fresh or conserved. Green mass can be used as a supplement to the daily protein ration of farming animals throughout the vegetation period, while the conserved alfalfa products, such as hay, silage, and flour, are a necessary supplement to the winter rations. It will certainly be used as protein food throughout the world in the near future, especially when low saponine cultivars have been created. At present, the sprouts produced from alfalfa are used in the USA, about six million pounds of seeds being used for this purpose annually (Hefterman and Teuber 1981).


Anther Culture Haploid Production Alfalfa Population Vitro Production Tetraploid Alfalfa 
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  1. Annual Report (1987) Dept of Agronomy, Ministerstvo na Zemedelie, Bulgaria, Sofia, p 88Google Scholar
  2. Bingham ET (1968) 'D'ansfer of diploid Medicago spp. germplasm to tetraploid M sativa L. in 4x - 2x crosses. Crop Sci 8:760-762Google Scholar
  3. Bingham ET (1971) Isolation of haploids of tetraploid alfalfa. Crop Sci 11: 433 - 435CrossRefGoogle Scholar
  4. Blaydes DF (1966) Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol Plant 19: 748 - 755CrossRefGoogle Scholar
  5. Chen Y (1986) Anther and pollen culture of rice. In: Hu H, Yang H (eds) Haploids of higher plants in vitro. Springer, Berlin Heidelberg New York, pp 3 - 25Google Scholar
  6. Chen CC, Kasha KJ, Marsolais A (1984) Segmentation patterns and mechanisms of genome multiplication in cultured microspores of barley. Can J Genet Cytol 26:475-483 Clarke JL (1851) Phil Trans, p 141Google Scholar
  7. Clement WM, Lehman WF (1963) Fertility and cytological studies of a diploid plant of alfalfa, Medicago sativa L. Crop Sci 2: 451 - 453CrossRefGoogle Scholar
  8. Davis WH (1967) Alfalfa hybrids. Minutes 10th Central Alfalfa Improvement Conf, pp 29 - 33Google Scholar
  9. Davis WH (1971) Hybrids alfalfa production. USA Patent No. 3570181Google Scholar
  10. Dumas de Vaulx R, Chambonnet D, Pochard E (1981) Culture in vitro d'antheres de piment (Capsicum annuum L.): amelioration des taux l'obtention de plantes des differents genotypes par des traitements a+35°C. Agronomie 1: 859 - 864CrossRefGoogle Scholar
  11. Encheva Y, Zagorska N (1985) Producing of haploids and their use in tobacco breeding. Proc Youth geneticists, Sofia 155 - 158Google Scholar
  12. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50: 151 - 158PubMedCrossRefGoogle Scholar
  13. Gomori G (1952) Microscopic histochemistry. Principles and practice, ChicagoGoogle Scholar
  14. Hesterman OB, Thuber LR (1981) Factors affecting yield and quality of alfalfa sprouts. Rep 27th alfalfa improvement Confer, USDA mannual AR U-NC-19, p 54Google Scholar
  15. Huang B (1986) Ultrastructural aspects of pollen embryogenesis in Hordeum, Triticum and Paeonia. In: Hu H, Yang H (eds) Haploids of higher plants in vitro. Springer, Berlin Heidelberg New York, pp 91 - 117Google Scholar
  16. Huang B, Sunderland N (1982) Thmperature-stress pretreatment in barley anther culture. Ann Bot (London) 49: 77 - 88Google Scholar
  17. Keller WA, Armstrong KC (1983) Production of haploids via anther culture in Brassica oleracea var. italica. Euphytia 32: 151 - 159CrossRefGoogle Scholar
  18. Lazar MD, Schaeffer GW, Baensiger PS (1985) The physical environment in relation to high frequency callus and plantlet development in anther cultures of wheat (Triticum saestivum L). cv. Chris. JGoogle Scholar
  19. Plant Physiol 121:103-109Google Scholar
  20. Lesins K (1957) Cytogenetic study on a tetraploid plant at the diploid chromosomal level. Can J Bot 35: 181 - 196CrossRefGoogle Scholar
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissueGoogle Scholar
  22. culture. Physiol Plant 15:473-497Google Scholar
  23. Niizeki H, Oono K (1971) Rice plants obtained by anther culture. Les cultures de tissue de plantes. Coli Int CNRS 193: 251-257Google Scholar
  24. Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19: 389 - 404Google Scholar
  25. Saunders JW, Bingham ET (1972) Production of alfalfa plants from callus tissue. Crop Sci 12: 804 - 808CrossRefGoogle Scholar
  26. Stanford EH, Clement WM (1958) Cytology and crossing behavior of a haploid alfalfa plant. Agric J 50: 589 - 592Google Scholar
  27. Stereva RA (1981) Male sterility in alfalfa. Genet Plant Breed 14 (6): 459 - 462Google Scholar
  28. Stereva RA, Kruleva MM, Gancheva V (1988) Investigation of two CMS alfalfa sources and their use in breeding program. Sbornic "100 years of Sadovo Exp Sta". Sadova, Bulgaria, pp 97 - 100Google Scholar
  29. Xu S (1979) Success of induction of pollan plants in alfalfa. Plant Mag 6: 5Google Scholar
  30. Zagorska N, Pandeva R (1986) In vitro androgenesis in Lycopersicon, Medicago and Capsicum. 6th Int Congr Plant tissue and cell culture, Minnesota, p 123Google Scholar
  31. Zagorska N, Robeva P, Dimitrov B, Stereva R, Gancheva V (1984) Induction of regeneration in anther cultures in Medicago sativa L. CR Acad Bulg Sci 37,8: 1099 - 1102Google Scholar
  32. Zagorska N, Abadjieva M, Oanh HK (1986) Factors affecting callus and plant production in anther cultures of tomato. In: Horn W, Jensen C, Odenbach W, Schieder 0 (eds) Genetic manipulationin plant breeding. De Gruyter, Berlin New York, pp 361 - 364Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • N. Zagorska
  • R. Stereva
  • P. Robeva
    • 1
  1. 1.Institute of GeneticsBulgarian Academy of SciencesBulgaria

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