Arabidopsis thaliana (L.): In Vitro Production of Haploids

  • R. L. Scholl
  • K. A. Feldmann
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 12)


Arabidopsis thaliana (L.) Heynh. is a small crucifer which grows wild in both the eastern and western hemispheres. While this plant has not been used for commercial purposes, it is well studied genetically. This is so because it possesses a very short generation time, and large populations of the species can be grown in confined areas. The biology (Napp-Zinn 1969; Kranz 1976) and genetics (Redei 1975) of A. thaliana have been reviewed. Tissue culture techniques of A. thaliana including callus culture (Yokayama and Jones 1965; Ziebur 1965; Negrutiu et al. 1978 a), suspension culture (Negrutiu et al. 1975; Lazar 1976), and shoot and root regeneration (Corcos et al. 1973; Feldmann and Marks 1986) have been well enough established to allow reliable in vitro manipulation of the species (Negrutiu et al. 1975; Negrutiu and Jacobs 1977).


Arabidopsis Thaliana Callus Induction Induction Medium Callus Culture Anther Culture 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • R. L. Scholl
    • 1
  • K. A. Feldmann
    • 2
  1. 1.Department of GeneticsThe Ohio State UniversityColumbusUSA
  2. 2.E.I. duPont de NemoursWilmingtonUSA

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