Hyoscyamus spp.: Anther Culture Studies

  • V. Raghavan
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 12)


The production of plants with the haploid or gametic number of chromosomes constitutes a significant application of tissue culture methodology for crop improvement. Haploid plants are regenerated when anthers at an appropriate stage of development are cultured in a nutrient medium with or without a proper balance of hormonal substances. The great interest in the production of haploids by anther or pollen culture techniques lies in the ease of methodology for obtaining haploids in large numbers, in the speeding of homozygosity, in the selection of desirable genetic combinations by doubling of chromosomes, in the production of mutants from cell cultures and in protoplast isolation and somatic hybridization.


Generative Cell Anther Culture Haploid Plant Tropane Alkaloid Pollen Culture 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • V. Raghavan
    • 1
  1. 1.Department of BotanyThe Ohio State UniversityColumbusUSA

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