Abstract
The putative horizontal transfer of foreign genes from transgenic plants to infecting wildtype agrobacteria has been examined. As a first step, the ability of Agrobacteria to take up foreign DNA via transformation has been analysed using different conditions. It could be confirmed that neither the presence of plant material nor the induction of virulence genes influenced the transformation frequency which was found to be very low in any case. Accordingly, in spite of the close contact of plants and Agrobacteria during the infection, no transfer of foreign genes could be traced. In conclusion, no wide-spread distribution of transgenes from plants to Agrobacteria in the free living can be expected.
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References
Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acid Res 12: 8711–8721
Bryngelsson NT, Gustafson M, Green B, Cannon F (1988) Uptake of host-DNA by the parasitic fungus Plasmodiophora brassicae. Physiol Mol Plant Pathol 33: 163–171
Heinemann JA, Sprague GF (1989) Bacterial conjugative plasmids mobilize DNAtransfer between bacteria and yeast. Nature 340: 205–209
Herrera-Estrella L, De Block M, Messens E, Hernalsteens JP, van Montagu M, Schell J (1983) Chimaeric genes as dominant selectable markers in plant cells. EMBO J 2: 987–995
Hoffmann T, Glotz C, Schieder O (1992) Preliminary evidence for horizontal gene transfer between higher plants and Aspergillus niger. In: Casper R, Landsmann J (eds) The biosafety results of field tests of genetically modified plants and microorganisms. Biologische Bundesanstalt für Land und Fortwirtschaft, Braunschweig, Germany, pp 247–249
Holsters M, de Waehle D, Depicker A, Messens E, van Montagu M, Schell J (1978) Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163: 181–187
Jin S, Komari T, Gordon MP, Nester EW (1987) Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281. J Bact 10: 4417–4425
Mogilner N, Zutra D, Gafny R, Bar-Joseph M (1993) The persistence of genetically engineered Agrobacterium tumefaciens in agroinfected plants. MPMI 6–5: 673–675
Ow D, Wood K, DeLucca M, DeWett J, Hdinski D, Howell S (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 342: 856–859
Stotzky G, Dcvanas MA, Zeph LR (1990) Methods for studying bacterial gene transfer in soil by conjugation and transduction. Adv Appl Microbiol 35: 57–169
van der Hoeven C, Dietz A, Landsmann J (1991) Utente Agrobakterien in transgenen Pflanzen nachgewiesen. Nachrichtenblatt Deutscher Pflanzenschutz 43: 249–251
Zambrysky PC (1992) Chronicles from the Agrobacterium-plant cell DNA transfer story. Annu Rev Plant Physiol Plant Mol Biol 43: 465–490
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© 1996 Springer-Verlag Berlin Heidelberg
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Broer, I., Dröge-Laser, W., Gerke, M. (1996). Examination of the Putative Horizontal Gene Transfer from Transgenic Plants to Agrobacteria . In: Schmidt, E.R., Hankeln, T. (eds) Transgenic Organisms and Biosafety. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61460-6_5
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DOI: https://doi.org/10.1007/978-3-642-61460-6_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-61077-9
Online ISBN: 978-3-642-61460-6
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