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Manufacture of Proteins Based on Recombinant Chinese Hamster Ovary Cells: Assessment of Genetic Issues and Assurance of Consistency and Quality

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Transgenic Organisms and Biosafety

Abstract

Chinese Hamster Ovary (CHO) cells are a popular mammalian host for the production of complex proteins for clinical applications. Today, more than 60% of candidate proteins for therapeutic and prophylactic purposes are being produced in mammalian cells, and CHO cells have served as pioneering hosts for recombinant cell culture technology on a large scale. Although other systems (prokaryotes, yeast, insects) are capable of producing large amounts of protein, the decision to employ mammalian cells has been driven by the need to obtain material with multiplicities in biological activity. These complex characteristics often depend on a number of post-translational events, i.e. correct folding and disulfide bridge formation, oligomerization, proteolytic processing, the addition of carbohydrate groups, secretion into the culture medium and more. Such activities are mediated, generally without major problems, by the protein producing machinery of mammalian cells. Reliable, high level, high quality and consistent productivity of cells in culture has been achieved through the development and use of novel techniques derived from many fields over the last 10 years. They range, to mention a few here, from the specifics of expression vectors to the complex biochemical composition of optimized cell culture media for large scale production processes and the engineering and design of adequate cell culture reactor equipment.

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Authors and Affiliations

  1. Départment de Chimie, Biotech Chem, IV, Ecole Polytechnique Fédérate de Lausanne EPFL, 1015, Lausanne, Switzerland

    F. M. Wurm

  2. Department of Process Sciences, Genentech Inc, South San Francisco, CA, 94080, USA

    C. J. Petropoulos & J. V. O’Connor

Authors
  1. F. M. Wurm
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  2. C. J. Petropoulos
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  3. J. V. O’Connor
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Editors and Affiliations

  1. Institut für Molekulargenetik, Gentechnologische Sicherheitsforschung und Beratung, Johannes Gutenberg-Universität Mainz, Johannes-J.-Becher-Weg 32, 55099, Mainz, Germany

    Erwin R. Schmidt  & Thomas Hankeln  & 

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© 1996 Springer-Verlag Berlin Heidelberg

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Wurm, F.M., Petropoulos, C.J., O’Connor, J.V. (1996). Manufacture of Proteins Based on Recombinant Chinese Hamster Ovary Cells: Assessment of Genetic Issues and Assurance of Consistency and Quality. In: Schmidt, E.R., Hankeln, T. (eds) Transgenic Organisms and Biosafety. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61460-6_28

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  • DOI: https://doi.org/10.1007/978-3-642-61460-6_28

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-61077-9

  • Online ISBN: 978-3-642-61460-6

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