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Circular RNAs: Generation of Small RNAs with Unique Properties by Splicing Permuted Intron-Exon Sequences

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Book cover Catalytic RNA

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 10))

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Abstract

Methods that utilize RNA splicing reactions to circularize RNA in vitro and in cells are being developed. These methods should facilitate in vitro biochemical and biophysical studies using circularized RNA and could eventually allow the expression of desired RNA sequence as circles in vivo. Circularized RNAs have proven useful for innovative in vitro studies on RNA structure and function (Pan and Uhlenbeck 1992a; Wang and Kool 1994). In addition to their value in biochemical studies, there is potential for the application of circular RNAs in the modulation of gene expression and in the developing area of nucleic acid-based therapeutics (Rossi et al. 1991; Ma et al. 1993; Wang and Kool 1994). However, with a few exceptions, naturally occurring examples of circular RNAs are rare, and little is known about their generation or fate in vivo.

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Authors and Affiliations

  1. Department of Biochemistry, Duke University Medical Center, Durham, NC, 27710, USA

    M. D. Been & M. Puttaraju

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  1. M. D. Been
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  2. M. Puttaraju
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Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, DD1 4HN, Dundee, UK

    David M. J. Lilley

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© 1996 Springer-Verlag Berlin Heidelberg

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Been, M.D., Puttaraju, M. (1996). Circular RNAs: Generation of Small RNAs with Unique Properties by Splicing Permuted Intron-Exon Sequences. In: Eckstein, F., Lilley, D.M.J. (eds) Catalytic RNA. Nucleic Acids and Molecular Biology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61202-2_8

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  • DOI: https://doi.org/10.1007/978-3-642-61202-2_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-62679-4

  • Online ISBN: 978-3-642-61202-2

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