Abstract
Little is known about RNA dynamics, even though it is likely that dynamics are important for both folding and function. The ribozyme, L-21 ScaI, derived from the group I intron of Tetrahymena thermophila (Zaug et al. 1988; Kay and Inoue 1987) provides an excellent system for studying dynamics, since its secondary structure is known (Michel and Dujon 1983; Burke et al. 1987; Cech et al. 1994) and a good model is available for its three-dimensional structure (Michel and Westhof 1990). Moreover, spectroscopic probes have been developed that are sensitive to binding of substrate by this ribozyme (Sugimoto et al. 1989b; Bevilacqua et al. 1992; Kierzek et al. 1993). This permits detection of intermediates and measurement of rate constants for various interconversions. The effects of substitutions and of solution conditions on these rate constants give insights into relationships between structure and dynamics and function. The RNA motion most intensively studied thus far in this system is docking of substrate into the catalytic core of the ribozyme (Bevilacqua et al. 1992, 1993, 1994; Li et al. 1995; Li, Profenno and Turner, unpubl. results). This chapter reviews the methods and results of these studies, and discusses some future perspectives.
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© 1996 Springer-Verlag Berlin Heidelberg
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Turner, D.H., Li, Y., Fountain, M., Profenno, L., Bevilacqua, P.C. (1996). Dynamics of a Group I Ribozyme Detected by Spectroscopic Methods. In: Eckstein, F., Lilley, D.M.J. (eds) Catalytic RNA. Nucleic Acids and Molecular Biology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61202-2_2
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DOI: https://doi.org/10.1007/978-3-642-61202-2_2
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