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Catalytic RNA pp 173–196Cite as

The Hammerhead Ribozyme

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Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 10))

Abstract

It was 10 years ago that the hammerhead ribozyme catalytic RNA motif was first detected in certain small satellite and viroid RNAs (for reviews see Symons 1992 and Bratty et al. 1993). Its two-dimensional representation (Fig. 1) has now been transformed into a three-dimensional model by the application of X-ray crystallography (Pley et al. 1994; Scott et al. 1995) (see also chapter by McKay), fluorescence resonance energy transfer (FRET) measurements (Tuschl et al. 1994), gel electrophoresis (Bassi et al. 1995), and transient electric birefringence measurements (Amiri and Hagerman 1994). In the past decade, the potential of ribozymes for the inhibition of gene expression has been demonstrated (for a review see Marschall et al. 1994). Particular examples are discussed in the chapters by Arndt and Atkins, Bertrand and Rossi, Sproat, Sun et al., Welch et al., and Usman and Stinchcomb, this Vol. This application has been successfully extended to the study of transgenic animals containing a hammerhead ribozyme gene targeted against the gene of interest (Heinrich et al. 1993; Zhao and Pick 1993; Efrat et al. 1994; Larsson et al. 1994; see also L’Huillier, this Vol.).

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  1. Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Strasse 3, 37075, Göttingen, Germany

    J. B. Thomson, T. Tuschl & F. Eckstein

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  1. J. B. Thomson
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  2. T. Tuschl
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  3. F. Eckstein
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  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, DD1 4HN, Dundee, UK

    David M. J. Lilley

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Thomson, J.B., Tuschl, T., Eckstein, F. (1996). The Hammerhead Ribozyme. In: Eckstein, F., Lilley, D.M.J. (eds) Catalytic RNA. Nucleic Acids and Molecular Biology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61202-2_10

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