Abstract
The effect of bacterial endotoxin (LPS) on the intracellular distribution of [3H]-inositol in human monocytic cells was studied. U937 cells were exposed to retinoic acid (RA) which has been shown to induce differentiation towards a monocytic phenotype. Inositol distribution in normal peripheral blood monocytes (Mø) was also studied as a comparison. Incubation of RA-treated U937 cells and blood Mø with LPS was followed by increased levels of [3H]-inositol phosphates and a decrease in [3H]-inositol incorporation into organic compounds. In parallel, retinoic acid upregulated the expression of the monocytic cell-surface marker CD14 in U937 cells. It is not certain that the onset of responsiveness to LPS in the generation of inositol phosphates and the increased expression of CD14 are related. However, it would appear that RA-treated U937 cells may be a useful model of blood monocytes for in vitro experimentation.
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© 1996 Springer-Verlag Berlin Heidelberg
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Ménager, N., Rotondo, D. (1996). Effect of Retinoic Acid on CD14 Expression and the Intracellular Distribution of Inositol Following LPS-Stimulation in U937 Cells. In: Tsiftsoglou, A.S., Sartorelli, A.C., Housman, D.E., Dexter, T.M. (eds) Tumor Biology. NATO ASI Series, vol 99. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61180-3_18
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DOI: https://doi.org/10.1007/978-3-642-61180-3_18
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