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Directed Gene Trapping in Embryonic Stem Cells

  • Conference paper
Gene Technology

Part of the book series: NATO ASI Series ((ASIH,volume 94))

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Abstract

Embryonic stem (ES) cell lines isolated from the inner cell mass of the mouse blastocyst (Evans & Kaufman, 1983), can be maintained as pluripotent stem cells and genetically manipulated in vitro. When reintroduced into a host embryo they can contribute to all tissues, including the germline of the resultant chimera (Beddington & Robertson, 1989). ES cells have provided the essential tool in the genetic manipulation of the mouse genome whereby an alteration to an allele of a loci can be made in vitro, introduced into the germline and then bred to homozygosity where the phenotypic effect can be examined.

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References

  • Beddington, R.S.P. & Robertson, E.J. (1989) An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo. Development 105: 733–737.

    PubMed  CAS  Google Scholar 

  • Conlon, R.A. & Rossant, J. (1992) Exogenous retinoic acid rapidly induces anterior ectopic expression of murine Hox-2 genes in vivo. Development 116: 357–368.

    PubMed  CAS  Google Scholar 

  • Doetschman, T., Eistetter, H., Katz, M., Schmidt, W. & Kemler, R. (1985). The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. exp. Morph. 87:27–45.

    PubMed  CAS  Google Scholar 

  • Eichele, G. (1989) Retinoids: signalling molecules in vetrebrate pattern formation. Trends Genet. 5: 246–251.

    Article  PubMed  CAS  Google Scholar 

  • Evans, M.J. & Kaufman, M.H. (1981) Establishment in culture of pluripotential cells from the mouse. Nature 292: 154–156.

    Article  PubMed  CAS  Google Scholar 

  • Freidrich, G. & Soriano, P. (1991) Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes & Dev. 5: 151

    Article  Google Scholar 

  • Gossler, A., Joyner, A., Rossant, J. & Skarnes, W.C. (1989) Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244:463–465.

    Article  PubMed  CAS  Google Scholar 

  • Hill & Wurst, W. (1993) Screening for Novel Pattern Formation Genes Using Gene Trap Approaches. Methods in Enzymology 225: 664–681.

    Article  PubMed  Google Scholar 

  • Nagy, A., Rossant, J., Nagy, R., Abramow-Newerly, W. & Roder, J.C. (1993). Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl. Acad. Sci. USA 90: 8424–8428.

    Article  PubMed  CAS  Google Scholar 

  • Simeone, A., Acampora, D., Arciono, L., Andrews, E., Boncinelli, E. & Mavilio, F. (1990) Sequential activation of HOX2 homeobox genes by retinoic acid in human embryonal carcinoma cells. Nature 346: 763–766.

    Article  PubMed  CAS  Google Scholar 

  • Simeone, A. Acampora, D., Nigoro, V., Faiella, A., D’Esposito, M., Stornaiuolo, A., Mavilio, F. & Boncinelli, E. (1991) Differential regulation by retinoic acid of the homeobox genes of the four HOX loci in human carcinoma cells. Mech. Dev. 33: 215–228.

    Article  PubMed  CAS  Google Scholar 

  • Skarnes, W.C. (1993). The identification of new genes: Gene trapping in Transgeneic Mice.. Current Opinion in Biotechnology 4:684–689.

    Article  PubMed  CAS  Google Scholar 

  • Skarnes, W.C., Auerbach, A.B. & Joyner, A.L. (1992). A gene trap approach in mouse embryonic stem cells: the lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev. 6: 903–918.

    Article  PubMed  CAS  Google Scholar 

  • Vasios, G., Mader, S., Gold, J.D., Leid, M., Lutz, Y., Gaub, M-P., Chambon, P. & Gudas, L.J. (1991) The late retinoic acid induction of laminin B1 gene transcription involves RAR binding to the responsive element. EMBO J. 10: 1149–1158.

    PubMed  CAS  Google Scholar 

  • Wiles, M.V. & Keller, G. (1991) Multiple haematopoietic lineages develop from embryonic stem (ES) cells in culture. Development 111: 259–267.

    PubMed  CAS  Google Scholar 

  • Wurst, W. & Joyner, A.L. (1993). Production of targeted embryonic stem cell clones. In: Gene Targeting, A Practical Approach, ed. A.L.Joyner, IRL Press, Oxford.

    Google Scholar 

  • Wurst, W., Rossant, J., Prideaux, V., Kownacka, M., Joyner, A.L., Hill, D.P., Guillemot, F., Gasca, S., Auerbach, A. & Ang, S-L. (1994). An embryonic expression screen for spatially restricted genes using a gene trap approach in ES cell chimeras. Genetics (in the press)

    Google Scholar 

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Author information

Authors and Affiliations

  1. Centre for Genome Research, University of Edinburgh, Kings Buildings, West Mains Rd, Edinburgh, Germany

    L.M Forrester

  2. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada

    A. Joyner, A. Bernstein & W. Wurst

  3. New York University Medical Centre, Skirbal Institute of Biomolecular Medicine, Developmental Genetics Programme, 540 First avenue, New York, New York, 10016, USA

    A. Joyner

  4. Institute fur Saugetiergenetik, Gesellschaft fur Strahlen und Umweltforschung mbH, Ingolstadter Landstr. 1, D-8042, Neuherberg, Germany

    W. Wurst

Authors
  1. L.M Forrester
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  2. A. Joyner
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  3. A. Bernstein
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  4. W. Wurst
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Editor information

Editors and Affiliations

  1. Universitätskrankenhaus Eppendorf, Martinistraße 52, D-20246, Hamburg, Germany

    Axel R. Zander

  2. Heinrich-Pette-lnstitut für Experimentelle Virologie und Immunologie, Universität Hamburg, Martinistraße 52, D-20246, Hamburg, Germany

    Wolfram Ostertag

  3. City Center for Advanced Medical Technologies, Bone Marrow Transplantation Center, Pr. Dynamo 3, 197042, St.Petersburg, Russia

    Boris V. Afanasiev

  4. Laboratory for Gene Structure Expression, Medical Research Council Institute, The Ridgeway, Mill Hill, London, NW71AA, UK

    Frank Grosveld

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© 1996 Springer-Verlag Berlin Heidelberg

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Forrester, L., Joyner, A., Bernstein, A., Wurst, W. (1996). Directed Gene Trapping in Embryonic Stem Cells. In: Zander, A.R., Ostertag, W., Afanasiev, B.V., Grosveld, F. (eds) Gene Technology. NATO ASI Series, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61122-3_9

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  • DOI: https://doi.org/10.1007/978-3-642-61122-3_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64704-8

  • Online ISBN: 978-3-642-61122-3

  • eBook Packages: Springer Book Archive

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