Summary
Retroviral insertional mutagenesis in vivo has proven to be an exquisite tool to identify previously unknown genes that are involved in tumorigenesis. Limitations of an in vivo system, e.g. selection for only one particular phenotype, can be overcome by in vitro insertional mutagenesis, which allows a variety of selection procedures for different phenotypes. In this study, we show that insertional mutagenesis can be used to define genes involved in receptor expression. For this purpose we used the murine hematopoietic cell line FDC-P1M that requires IL-3 for growth but does not normally respond to GM-CSF. Following retroviral insertional mutagenesis, mutants were selected that were either factor independent or had acquired the capacity to proliferate in response to GM-CSF. We describe here the phenotype of the GM-CSF responsive mutants and demonstrate the activation of the GM-CSF receptor a-chain responsible for the altered growth factor response.
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© 1996 Springer-Verlag Berlin Heidelberg
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Laker, C., Friel, J., Franz, MJ., Baccarini, M., Ostertag, W., Stocking, C. (1996). Altered Growth Factor Response in Myeloid Progenitor Cell Mutants Derived after Retroviral Insertlonal Mutagenesis. In: Zander, A.R., Ostertag, W., Afanasiev, B.V., Grosveld, F. (eds) Gene Technology. NATO ASI Series, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61122-3_22
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DOI: https://doi.org/10.1007/978-3-642-61122-3_22
Publisher Name: Springer, Berlin, Heidelberg
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