Abstract
When functioning normally, oncosuppressor genes, also known as tumour suppressor genes or antioncogenes, prevent the development of one or more types of cancer (reviewed by Knudson, 1993). A powerful way of investigating the mechanism of action of such genes is to examine the consequences of inactivating them in an experimental animal. This can be done via gene targeting in embryonal stem (ES) cells (reviewed by Hooper, 1992). These cells, established in culture from the pluripotent inner cell mass cells of the peri-implantation embryo, retain the capacity to colonise the somatic tissues and germ line of chimaeras produced by injecting them into blastocyst-stage embryos. Tailored mutations can be introduced into chosen genes by introducing into the cultured cells a gene targeting DNA vector and isolating cells in which the vector has undergone homologous recombination with the endogenous gene. These mutations can then be introduced via chimaeras into the mouse germ line. Here I review the study of the Rb-1 and p53 oncosuppressor genes using this approach.
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© 1996 Springer-Verlag Berlin Heidelberg
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Hooper, M.L. (1996). Analysis of Oncosuppressor Gene Function in the Mouse by Gene Targeting. In: Zander, A.R., Ostertag, W., Afanasiev, B.V., Grosveld, F. (eds) Gene Technology. NATO ASI Series, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61122-3_19
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DOI: https://doi.org/10.1007/978-3-642-61122-3_19
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