Abstract
Retroviral vectors designed to transduce hemopoietic stem cells have to be optimised with respect to basic requirements for gene transfer and expression, (i) The cis-regulatory elements of the genomic vector RNA interacting with retroviral proteins supplied from packaging cells must allow for optimal packaging, reverse transcription and integration into the genome of the target cell, (ii) The cis-regulatory elements of the integrated ’proviral’ vector DNA interacting with the cellular transcriptional machinery must guarantee efficient and long-lasting gene expression in the target cell and its progeny.
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© 1996 Springer-Verlag Berlin Heidelberg
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Baum, C., Eckert, HG., Ostertag, W. (1996). Contribution of the Retroviral Leader to Gene Transfer and Expression in Packaging Cells and Myeloid Stem and Progenitor Cells. In: Zander, A.R., Ostertag, W., Afanasiev, B.V., Grosveld, F. (eds) Gene Technology. NATO ASI Series, vol 94. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61122-3_16
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DOI: https://doi.org/10.1007/978-3-642-61122-3_16
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