Abstract
Current concepts in cell biology suggest that there are checkpoints in the various phases of the cell cycle at which cells are monitored for damage to their DNA. Studies in our laboratory on the effect of radiation on cells in culture have shown delay of progression in both the G1 and G2 phases of the cell cycle of normal, non-transformed cells. It has been proposed that the delay period in these phases allows time for cells to repair DNA damage before proceeding to the next phase of the cell cycle. Thus cells in G1 would be required to repair potential DNA damage prior to entering S phase in order to alleviate the possibility of replicating an abnormal and potentially malignant genome. Studies on the regulations at the proposed checkpoints are important for elucidating cell cycle control mechanisms. Such investigations require techniques that can continuously monitor the distribution of cell throughout the cell cycle and access the proliferation potential of cells in different phases of the cell cycle.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Begg AC, McNally NJ, Shrieve DC, Karcher HA (1985). A method to measure the duration of DNA synthesis and the potential doubling time from a single sample Cytometry 6:620–626.
Bohmer RM (1979). Flow cytometric cell-cycle analysis using the quenching of 33258 Hoechst fluorescence by bromodeoxyuridine incorporation. Cell Tissue Kinet. 12:101–110.
Bohmer RM, Eilwart J (1981) Combination of BUdR-quenched Hoechst fluorescence with DNA-specific ethidium bromide fluorescence for cell cycle analysis with a two-parameter flow cytometer. Cell Tissue Kinet. 14: 653–658.
Crissman HA, Gadbois DM, Tobey RA, Bradbury EM. (1991) Transformed mammalian cells are deficient in kinase-mediated control of progression through the G1 phase of the cell cycle. Proc. Natl Acad. Sei, USA 88–7580–7584.
Crissman HA, Steinkamp JA (1987) A new method for rapid and sensitive detection of bromodeoxyuridine in DNA replicating cells. Exp. Cell Res. 173: 256–261.
Crissman HA, Steinkamp JA (1990). Detection of bromodeoxyuridine-labeled cells by differential fluorescence analysis of DNA fluorochromes. In: Darzynkiewicz Z, Crissman HA (eds), Flow Cytometry. Methods in Cell Biology, Vol 33, San Diego: Academic Press, pp 199–206.
Crissman HA, Stevenson AP, Kissane RJ, and Tobey RA (1979). Techniques for quantitative staining of cellular DNA for flow cytometric analysis. In: “Flow Cytometry and Sorting” (Melamed MR, Mullaney PF, and Mendelsohn ML, eds), New York: John Wiley & Sons, pp 243–261.
Crissman HA, Tobey RA (1974). Cell cycle analysis in 20 minutes. Science 184: 1297–1298.
Dean PN, Jett JH (1974). Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol 60:523–527.
Dolbeare F, Gratzner H, Pallavacini M, and Gray JW (1983). Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sei USA 80: 5573–5577.
Dolbeare F, Gray JW (1988). Use of restriction endonucleases and exonuclease III to expose halogenated pyridines for immunochemical staining. Cytometry 9: 631–635.
Dolbeare F, Kuo W-L, Beisker W, Vanderlaan M, Gray JW (1990). Using monoclonal antibodies in bromodeoxyuridine-DNA analysis. In: Darzynkiewicz Z, Crissman HA (eds), Flow Cytometry. Methods in Cell Biology, Vol 33, San Diego: Academic Press, pp 207–216.
Ellwart J, Dohmer P (1985). Effect of 5-fluoro-2’-deoxyuridine (FdUrd) on 5-bromo- 2 deoxyuridine (BrdUrd) incorporation into DNA measured with a monoclonal BrdUrd antibody and by BrdUrd/Hoechst quenching effect. Cytometry 6: 513–520.
Gautier J, Minshull J, Lohka M, Glotzer M, Hunter T, Maller JL (1990). Cyclin is a component of maturation-promoting factor from Xenopus. Cell 60:487–494.
Gong J, Traganos F, Darzynkiewicz Z (1993). Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based in DNA content and cyclin B nfieasurements. Cancer Res 53:5096–5099.
GratZner H (1982). Monoclonal antibody against 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218: 474–475.
Habbersett R, Crissman HA, Jett JH (1990). BrdU incorporation detected by differential fluorescence and digital signal processing. Cytometry (Suppl. 4), Abstract 528 B. p88.
Kubbies M, Rabinovitch PS (1983). Flow cytometric analysis of factors which influence the BrdUrd Hoechst quenching effect in cultivated human fibroblasts and lymphocytes. Cytometry 3: 276–281.
Latt SA (1973). Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci USA 70: 3395–3399.
Li X, Traganos F, Melamed MR, Darzynkiewicz Z. Single step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides. Detection of apoptosis and BrdUrd incorporation. Cytometry, in press.
Murray AW, Kirschner MW (1989). Dominoes and clocks: the union of two views of the cell cycle. Science 246:614–621.
Poot M, Hoehn H, Kubbies M, Grossman A, Chen Y, Rabinovitch PS (1990). Cell cycle analysis using continuous bromodeoxyuridine labeling and Hoechst 33258-ethidium bromide bivariate flow cytometry. In: Darzynkiewicz Z, Crissman HA (eds), Flow Cytometry. Methods in Cell Biology, Vol 33, San Diego: Academic Press, pp 185–206.
Sherwood SW, Rush DF, Kung AL, Schimke RT (1994). Cyclin B1 expression in HeLa S3 cells studied by flow cytometry. Exp Cell Res 211:275–281.
Steinkamp JA, Stewart CC (1986). Dual laser, differential fluorescence correction method for reducing cellular background autofluorescence. Cytometry 7: 566–574.
Steinkamp JA., Stewart CC, Crissman H. A. (1982). Three-color fluorescence measurements on single cells excited at three laser wavelengths. Cytometry 2, 226–231.
Tobey RA., Oishi N., Crissman HA. (1989) Synchronized human diploid fibroblasts: Progression capabilities of a subpopulation that fails to keep pace with the predominant, rapidly dividing cohort of cells. J. Cell. Physiol. 139:432–440
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Crissman, H.A., Nastasi, A.J. (1996). Cell Cycle and Cell Proliferation Markers. In: Jacquamin-Sablon, A. (eds) Flow and Image Cytometry. NATO ASI Series, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61115-5_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-61115-5_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-64701-7
Online ISBN: 978-3-642-61115-5
eBook Packages: Springer Book Archive