Abstract
The studies described in this manuscript have been aimed to obtain a better insight into the cytotoxic interaction between human Natural Killer (NK) cells and K562 target cells1. In order to obtain information about crucial processes leading to target cell death, the processes taking place in the target cell during a killer cell attack were studied using different optical techniques. Furthermore, flow cytometric methods for determination of the phenotype and the cytotoxic activity of killer cells were developed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Reference
Radošević K, Garritsen HSP, Van Graft M, De Grooth BG, Greve J (1990) A simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells. J Immunol Methods 135:81–89.
Radošević K, Bakker Schut TC, Van Graft M, De Grooth BG, Greve J (1993a) A flow cytometric study of the membrane potential of natural killer and K562 cells during the cytotoxic process. J Immunol Methods 161:119–128.
Bakker Schut TC, Florians A, Radošević K, De Grooth BG, Greve J (1993) Signal processing in slit-scan flow cytometry of cell conjugates. Cytometry 14:459–464.
Radošević K, De Grooth BG, Greve J (1993b) Flow cytometric method for simultaneous detection of lymphocyte-K562 conjugates and immunophenotyping of the conjugate forming cells. Cytometry 14:535–540.
Radošević K, Van Leeuwen AMT, Greve J (1994a) CLSM of living cell conjugates. Eur Microsc Anal 28:41.
Radošević K, Van Leeuwen AMT, Segers-Nolten IMJ, Figdor CG, De Grooth BG, Greve J (1994b) Changes in actin organization during the cytotoxic process. Cytometry 15:320–326.
Radošević K, De Grooth, BG, Greve J (1995a) Changes in intracellular calcium concentration and pH of target cells during the cytotoxic process: a quantitative study at the single cell level. Cytometry, in press.
Radošević K, Van Leeuwen AMT, Segers-Nolten IMJ, Figdor CG, De Grooth BG, Greve J (1995b) The occurrence and a possible mechanism of penetration of natural killer cells into K562 target cells during the cytotoxic interaction. Cytometry, in press.An extended bibliography on the subjects described in this paper can be found in the corresponding references.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Radošević, K., de Grooth, B.G., Greve, J. (1996). Use of Different Cytometric Techniques to Study the Cytotoxic Interaction between Human Natural Killer Cells and k562 Target cells. In: Jacquamin-Sablon, A. (eds) Flow and Image Cytometry. NATO ASI Series, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61115-5_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-61115-5_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-64701-7
Online ISBN: 978-3-642-61115-5
eBook Packages: Springer Book Archive