Abstract
Flow cytometry is the best method for classifying leukemias and lymphomas (Braylan, 1989; Braylan, 1993; Deegan, 1989; Drexler, 1987; Duque, 1993; Foon, 1986; Neame, 1986; Stewart, 1989, 1990A, 1990B, Terstappen, 1988; Verwer, 1993; Weber, 1990; Wilman, 1989). In a flow cytometer, a distinct group of cells is identified by its repertoire of cell surface markers or epitopes. An epitope is labeled using a monoclonal antibody with an attached fluorochrome. A flow cytometer is used to detect the monoclonal antibody through its attached fluorochrome, which, in turn, identifies an epitope on a cell. Appropriate combinations of monoclonal antibodies provide a set of probes for labeling all important epitopes. This process enables the identification of cell types in a specimen. For three-color immunophenotyping, the set of three different probes is referred to as a panel. Each panel is designed to label a specific set of cell types. A panel of three probes provides more information about specific groups of cells than do panels of one or two probes. This additional information requires the use of more complex data analysis methods than are now available.
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© 1996 Springer-Verlag Berlin Heidelberg
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Salzman, G.C., Beckman, R.J., Parson, J.D., Nauman, A.M., Stewart, S.J., Stewart, C.C. (1996). Flow Cytometric Immunophenotyping Using Cluster Analysis And Cluster Editing. In: Jacquamin-Sablon, A. (eds) Flow and Image Cytometry. NATO ASI Series, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61115-5_15
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DOI: https://doi.org/10.1007/978-3-642-61115-5_15
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