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Flow and Image Cytometry pp 131–141Cite as

Molecular Cytogenetics: Uses of flow sorted chromosomes, fluorescence in situ hybridisation (FISH) and digital microscopy for the analysis of genomes

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Part of the book series: NATO ASI Series ((ASIH,volume 95))

Abstract

The application of molecular techniques to the study of chromosome structure and rearrangements has revolutionised cytogenetics. Recombinant DNA technology and flow sorted chromosome libraries now provide us with specific probes which, coupled with in situ hybridisation techniques, allow the direct visualisation of the position of DNA sequences on metaphase chromosomes and in interphase nuclei. While these techniques allow direct gene mapping and ordering, their application to clinical samples now enables precise diagnosis of chromosomal abnormalities.

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Author information

Authors and Affiliations

  1. The Sanger Centre, Hinxton Hall, Hinxton, Cambridge, CB10 1RQ, UK

    Nigel P. Carter

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  1. Nigel P. Carter
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Editors and Affiliations

  1. Centre National de le Recherche Scientifique, Laboratorie de Cytométrie (UPS 47), 7, rue Guy Moquet, F-94800, Villejuif, France

    Alain Jacquamin-Sablon

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© 1996 Springer-Verlag Berlin Heidelberg

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Carter, N.P. (1996). Molecular Cytogenetics: Uses of flow sorted chromosomes, fluorescence in situ hybridisation (FISH) and digital microscopy for the analysis of genomes. In: Jacquamin-Sablon, A. (eds) Flow and Image Cytometry. NATO ASI Series, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61115-5_10

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  • DOI: https://doi.org/10.1007/978-3-642-61115-5_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64701-7

  • Online ISBN: 978-3-642-61115-5

  • eBook Packages: Springer Book Archive

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