c-Myc Dependent Initiation of Genomic Instability During Neoplastic Transformation

  • C. Taylor
  • A. Jalava
  • S. Mai
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 224)


The dihydrofolate reductase (DHFR) gene is a target of c-Myc in genomic instability. The induced overexpression of c-Myc in cell lines is followed by the amplification and rearrangement of the DHFR gene. Furthermore, the constitutive upregulation of c-Myc protein coincides with genomic instability of the DHFR gene in lymphoid, non-lymphoid and in tumor lines. The amplification of the DHFR gene is locus-specific and independent of species origins. We have now addressed the question whether inducible deregulation of c-Myc is followed by DHFR gene amplification in vivo. We show that the DHFR gene is a target of c-Myc-dependent neoplasia in vivo and propose a role for genomic instability during the initiation of neoplastic transformation.


Gene Amplification Genomic Instability Dihydrofolate Reductase DHFR Gene Normal Diploid Cell 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • C. Taylor
    • 1
  • A. Jalava
    • 1
  • S. Mai
    • 1
  1. 1.Manitoba Institute of Cell Biology and the University of ManitobaWinnipegCanada

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