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A Biochemical and Biological Analysis of Myc Superfamily Interactions

  • N. Schreiber-Agus
  • L. Alland
  • R. Muhle
  • J. Goltz
  • K. Chen
  • L. Stevens
  • D. Stein
  • R. A. DePinho
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 224)

Abstract

Members of the myc family of nuclear proto-oncogenes (c-, N- and L-myc) play central roles in the control of normal growth and development and in genetic pathways linked to cellular transformation and apoptotic cell death (for reviews see [19, 40]). Accumulating structural, biochemical, and genetic evidence affords the view that the function of Myc family oncoproteins in these diverse processes relates in part to their roles as sequence-specific transcription factors (for reviews see [31, 58]). Myc family proteins possess a multi-functional amino-terminal domain with transactivation potential [32], a region rich in basic amino acid residues responsible for sequence-specific DNA binding activity to the E-box consensus CACGTG [8], and a carboxy-terminal α-helical domain required for dimerization with another basic region helix-loop-helix/leucine zipper (bHLH/LZ) protein, Max [9, 43]. Many of the biochemical and biological activities of Myc appear to be highly dependent upon its association with Max [1, 2, 9, 35, 43). In addition to its key role as an obligate partner in transactivation-competent Myc/Max complexes, Max may also repress Myc-responsive genes through the formation of transactivation-inert complexes that are capable of binding the Myc/Max recognition sequence [5, 10, 33, 35, 39, 41, 44, 65]. These complexes include Max/Max homodimers, and the heterodimers Mad/Max [5, 29] and Mxil/Max [65].

Keywords

Albert Einstein College Repression Domain Cell BioI Calcium Phosphate Precipitation Method Early Passage Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • N. Schreiber-Agus
    • 1
  • L. Alland
    • 1
  • R. Muhle
    • 1
  • J. Goltz
    • 2
  • K. Chen
    • 1
  • L. Stevens
    • 3
  • D. Stein
    • 2
  • R. A. DePinho
    • 1
  1. 1.Dept. of Microbiology and ImmunologyAlbert Einstein College of MedicineBronxUSA
  2. 2.Dept. of Molecular GeneticsAlbert Einstein College of MedicineBronxUSA
  3. 3.Dept. of Developmental and Molecular BiologyAlbert Einstein College of MedicineBronxUSA

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