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Neutrotransmitter Release and Uptake pp 101–118Cite as

Stimulation of Exocytosis in Cultured Cerebellar Granule Cells by Electrical Field Stimulation

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Part of the book series: NATO ASI Series ((ASIH,volume 100))

Abstract

External excitatory stimuli are required in order to evoke neurotransmitter release from cultured neurones. Traditionally, chemical stimuli have been employed to mimic synaptic input, with elevated KCl being the most widely used method to depolarize the plasma membrane. However, KCl stimulation has disadvantages in that the plasma membrane of the preparation is permanently clamped in a depolarized state allowing no repolarization to take place. In addition by definition, no modulation of K+ channels are able to be studied by this technique.

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Author information

Authors and Affiliations

  1. Neurosciences Institute, Department of Pharmacology, University of Dundee, Dundee, DD1 9SY, UK

    M. A. Cousin, H. Hurst & D. G. Nicholls

  2. Department of Pharmacology, University of Leeds, Leeds, UK

    B. Held

Authors
  1. M. A. Cousin
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  2. H. Hurst
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  3. B. Held
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  4. D. G. Nicholls
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Editors and Affiliations

  1. Center for Brain Research, Ege University, Bornova, Izmir, 35100, Turkey

    Şakire Pöğün

  2. Department of Physiology, Ege University School of Medicine, Bornova, Izmir, 35100, Turkey

    Şakire Pöğün

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© 1997 Springer-Verlag Berlin Heidelberg

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Cousin, M.A., Hurst, H., Held, B., Nicholls, D.G. (1997). Stimulation of Exocytosis in Cultured Cerebellar Granule Cells by Electrical Field Stimulation. In: Pöğün, Ş. (eds) Neutrotransmitter Release and Uptake. NATO ASI Series, vol 100. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60704-2_7

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