Abstract
The development of bipotential glial progenitor cells has been extensively studied using primary cell culture techniques (5, 8). Oligodendrocyte progenitors require multiple signals to proliferate in culture, after several cell cycles the cells stop dividing and in the absence of serum or in medium containing low concentrations of serum, the 0-2A cells differentiate spontaneously into oligodendrocytes. This process is characterized by the loss of intermediate filament protein vimentin and the cell surface antigen A2B5. Concomitantly, galactocerebroside (GC) appears on the cell surfaces and numerous branched cellular processes are extended, and lateron myelin specific proteins, i.e. Wolfgram protein (CNP), myelin basic protein (MBP), myelin associated glycoprotein (MAG), and proteolipid protein (PLP) are expressed even in the absence of neurons (5).
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Richter-Landsberg, C. (1997). OLN-Cells: a New Oligodendroglia Cell Line with Impact for Cell Transplantation. In: Jeserich, G., Althaus, H.H., Richter-Landsberg, C., Heumann, R. (eds) Molecular Signaling and Regulation in Glial Cells. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60669-4_30
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DOI: https://doi.org/10.1007/978-3-642-60669-4_30
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