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Mature Pig Astrocytes Do Not Respond to NGF as Oligodendrocytes Do

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Molecular Signaling and Regulation in Glial Cells

Abstract

Recently, it was shown that NGF enhances the process regeneration of mature pig oligodendrocytes (OL) and that it induces a subset of mature OL to proliferate. This effect is mediated by TrkA, the NGF tyrosine kinase receptor. Astrocytes belong to the cells in the CNS, which synthesise and secrete NGF, however, it is unknown whether pig astrocytes can also respond to NGF. Firstly, we established the cultural requirements for mature pig astrocytes. During culturing three different types of GFAP+ cells could be observed, of which the flat and the stellate type were predominant. NGF did neither influence the astroglial process formation nor did it alter the rate of the 3H-thymidine incorporation. An in gel kinase assay revealed that a key enzyme of one of the intracellular signaling cascades, Erkl, is functioning in astrocytes, however, its activity cannot be enhanced by NGF.

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Authors and Affiliations

  1. AG Neural Regeneration, Max-Planck Institute for Exp. Medicine, Göttingen, Germany

    Hans H. Althaus, Tyede Schmidt-Schultz & Sabine Klöppner

Authors
  1. Hans H. Althaus
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  2. Tyede Schmidt-Schultz
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  3. Sabine Klöppner
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Editor information

Editors and Affiliations

  1. FB Biologie/Chemie, Universität Osnabrück, Barbarastr. 11, D-49069, Osnabrück, Germany

    Gunnar Jeserich

  2. Max-Planck-Institut für Experimentelle Medizin, Hermann-Reinsstr. 3, D-37075, Göttingen, Germany

    Hans H. Althaus

  3. FB Biologie, Universität Oldenburg, Postfach 2503, D-26111, Oldenburg, Germany

    Christiane Richter-Landsberg

  4. Molekulare Neurobiochemie, Universität Bochum, Universitätsstr. 150, D-44801, Bochum, Germany

    Rolf Heumann

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© 1997 Springer-Verlag Berlin Heidelberg

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Althaus, H.H., Schmidt-Schultz, T., Klöppner, S. (1997). Mature Pig Astrocytes Do Not Respond to NGF as Oligodendrocytes Do. In: Jeserich, G., Althaus, H.H., Richter-Landsberg, C., Heumann, R. (eds) Molecular Signaling and Regulation in Glial Cells. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60669-4_3

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  • DOI: https://doi.org/10.1007/978-3-642-60669-4_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64501-3

  • Online ISBN: 978-3-642-60669-4

  • eBook Packages: Springer Book Archive

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