Abstract
The dedication of differentially expressed genes is an essential prerequisite in several areas of biomolecular research such as, for exampe, those pertaining to cell differentiation, development or neoplastic transformation. Most fundamental biological processes depend, in fact, on programs of differential gene expression, i.e., they are determined by the “choice” of which genes are expressed in a particular cell population at a given time.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
An G, Luo G, Veltri RW, O’Hara SM (1996) Sensitive, nonradioactive differential display method using chemiluminescent detection. BioTechniques 20:342 – 346
Bauer D, Müller H, Reich J, Riedel H, Ahrenkiel V, Warthoe P, Strauss M (1993) Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR). Nucleic Acids Res 21:4272–4280
Callard D, Lescure B, Mazzolini L (1994) A method for the elimination of false positives generated by the mRNA differential display technique. BioTechniques 16:1096–1103
Hakvoort TBM, Leegwater ACJ, Michiels FAM, Chamuleau RAFM, Lamers WH (1994) Identification of enriched sequences from a cDNA subtraction-hybridization procedure. Nucleic Acids Res 22:878–879
Liang P, Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967–971
Liang P, Pardee AB (1995) Recent advances in differential display. Curr Opin Immunol 7:274–280
Liang P, Averboukh L, Pardee AB (1993) Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res 21:3269–3275
Liang P, Zhu W, Zhang X, Gou Z, O’Connell RP, Averboukh L, Wang F, Pardee AB (1994) Differential display using one-base-anchored oligo-dT primers. Nucleic Acids Res 22:5763–5764
Liu C, Raghothama KG (1996) Practical method for cloning cDNAs generated in an mRNA differential display. Biotechniques 20:576–580
Lohmann J, Schickle H, Bosch TCG (1995) REN display, a rapid and efficient method for nonradioactive differential display and mRNA isolation. BioTechniques 18:200–202
McClelland M, Welsh J (1994) RNA fingerprinting by arbitrarily primed PCR. PCR Methods Applic 4:s66-s81
McClelland M, Ralph D, Cheng R, Welsh J (1994) Interactions among regulators of RNA abundance characterized using RNA fingerprinting by arbitrarily primed PCR. Nucleic Acids Res 22:4419–4431
McClelland M, Mathieu-Daude F, Welsh J (1995) RNA fingerprinting and differential display using arbitrarily primed PCR. Trends Genet 11:242–246
Miyashiro K, Dichter M, Eberwine J (1994) On the nature and differential distribution of mRNAs in hippocampal neurites: implications for neuronal functioning. Proc Natl Acad Sci USA 91:10800–10804
Ralph D, Welsh J, McClelland M (1993) RNA fingerprinting using arbitrarily primed PCR identifies regulated RNase in Mink lung (MvlLu) cells growth arrested by TGFß. Proc Natl Acad Sci USA 90:10710–10714
Rohrwild M, Alpan RS, Liang P, Pardee AB (1995) Inosine-containing primers for mRNA differential display. Trends Genet 11:300
Sargent TD, Dawid IB (1983) Differential gene expression in the gastrula of Xenopus laevis. Science 222:135–139
Shoham NG, Arad T, Rosin-Abersfeld R, Mashiah P, Gazit A, Yaniv A (1996) Differential display assay and analysis. BioTechniques 20:182–184
Sokolov BP, Prockop DJ (1994) A rapid and simple PCR-based method for isolation of cDNAs from differentially expressed genes. Nucleic Acids Res 22:4009–4015
St John T, Davis RW (1979) Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization. Cell 16:443–452
Stone B, Wharton W (1994) Targeted RNA fingerprinting: the cloning of differentially-expressed cDNA fragments enriched for members of the zinc finger gene family. Nucleic Acids Res 22:2612–2618
Welsh J, Chada K, Dalai SS, Cheng R, Ralph D, McClelland M (1992) Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res 20:4965–4970
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Zhao S, Ooi SL, Pardee AB (1995) New primer strategy improves precision of differential display. BioTechiques 18:842–850
Zhao S, Ooi SL, Yang FC, Pardee AB (1996) Methods for identification of true positive cloned cDNA fragments in differential display. BioTechniques 20:400–404
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Micheli, M.R., Bova, R. (1997). Overview. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_27
Download citation
DOI: https://doi.org/10.1007/978-3-642-60441-6_27
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47812-3
Online ISBN: 978-3-642-60441-6
eBook Packages: Springer Book Archive