Abstract
DNA fingerprinting methods based on PCR amplification using arbitrary primers such as RAPD (Williams et al. 1990), AP-PCR (Welsh and McClelland 1990) and DAF (Caetano-Anollés et al. 1991) require minimal amounts of DNA (generally a few ng per reaction); however, it has been shown that these methods may be significantly influenced by the quality of the template. The choice of an extraction procedure yielding high molecular weight DNA (free of contaminants which can interfere with the amplification process) is, therefore, an essential prerequisite in obtaining reliable DNA fingerprints. Furthermore, it is important that the same extraction procedure is used for all DNAs to be compared.
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References
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© 1997 Springer-Verlag Berlin Heidelberg
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D’Ambrosio, E., Pascale, E. (1997). DNA Extraction from Mammals. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_2
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DOI: https://doi.org/10.1007/978-3-642-60441-6_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47812-3
Online ISBN: 978-3-642-60441-6
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