Abstract
Cloning of RAPD markers is a valuable technique for the study and utilization of RAPD amplification products. It can contribute to the characterization of a DNA region that is species- or group-specific, allowing the construction of probes and oligonucleotides to be used for the detection of microorganisms (see Chap. XXV). Moreover cloning of RAPD markers is an important step in procedures to determine the nucleotide sequence of the marker (see Chap. XIX). Sequencing could be used to identify and study the regions of a target genome amplified in RAPD experiments. This may help in determining whether there are some regions of the bacterial genome that are preferentially amplified during RAPD. In addition, the identification of RAPD fragments may highlight the nucleotide sequence of primer annealing sites and may help in understanding the molecular mechanism generating RAPD patterns.
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References
Fani R, Damiani G, Di Serio C, Gallon E, Grifoni A, Bazzicalupo M (1993) Use of random amplified polymorphic DNA (RAPD) for generating specific DNA probes for microorganisms. Mol Ecol 2:243–250
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© 1997 Springer-Verlag Berlin Heidelberg
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Mori, E., Fani, R. (1997). Cloning of RAPD Markers. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_19
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DOI: https://doi.org/10.1007/978-3-642-60441-6_19
Publisher Name: Springer, Berlin, Heidelberg
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