High Throughput Scoring of RAPD Fragments Through the Use of Dot-Blot Hybridization

Abstract

The application of marker assisted selection to plant breeding is constrained by the cost of the technology employed and throughput capacity. At present, labor is a much more significant component of the cost per reaction than consumables. The reliance of PCR based marker methods on gel electrophoresis limits the number of samples that can be reasonably processed per unit of labor. Recent improvements in the detection of polymorphic fragments through denaturing acrylamide techniques such as denaturing gradient gel electrophoresis (DGGE) (see Chap. XIV) and temperature sweep gel electrophoresis (TSGE) (see Chap. XV) are not readily amenable to large scale, marker screening programs.