Abstract
DNA-based marker systems have, over the past 5 years, become much more refined as adaptations are incorporated to address needs specific to different genetic systems. PCR-based methodologies have found important applications in genomic mapping efforts due to the small amounts of tissue required and the rapidity of the procedure; these advantages allow the investigator to conduct studies with much larger population sizes than were previously feasible. Elaborations on the standard PCR procedure have facilitated the simultaneous evaluation of exceedingly large numbers of loci per experiment (Vos et al. 1995) as well as the rapid screening for linkage disequilibrium in segregating or heterogeneous populations (Michelmore et al. 1991). Such approaches have proven invaluable for high density mapping of a target genomic region.
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© 1997 Springer-Verlag Berlin Heidelberg
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Dweikat, I., Mackenzie, S. (1997). Denaturing Gradient Gel Electrophoresis for Enhanced Detection of DNA Polymorphisms. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_15
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DOI: https://doi.org/10.1007/978-3-642-60441-6_15
Publisher Name: Springer, Berlin, Heidelberg
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