Abstract
The amplification of nucleic acid templates with one or more arbitrary oligonucleotide primer produces specific signatures that can be used to study virtually any nucleic acid whether anonymous in nature or previously characterized. This multiple arbitrary amplicon profiling (MAAP) strategy lends itself to the study of complex genomes in comparative and experimental biology applications that range from molecular systematics to genetic mapping (Caetano-Anollés 1994). It can also be applied to the fingerprinting of cDNA populations, PCR products, extrachromosomal nucleic acids, and cloned DNA. However, in many nucleic acid scanning applications there is a need to tailor the performance of the amplification reaction. For example, in molecular ecology and evolution there is sometimes a requirement to increase or decrease the ability to distinguish a group of organisms. Generally, this is done by using more than one fingerprinting technique capable of resolving genomes, for example, at the species or subspecies level. The versatility of nucleic acid scanning has overcome some of these limitations. The concept of “fingerprint tailoring” was introduced some time ago to depict those strategies which modify fingerprint pattern (Caetano-Anollés et al. 1991).
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Caetano-Anollés, G. (1997). Fingerprint Tailoring. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_13
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DOI: https://doi.org/10.1007/978-3-642-60441-6_13
Publisher Name: Springer, Berlin, Heidelberg
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