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DNA Amplification Fingerprinting

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Fingerprinting Methods Based on Arbitrarily Primed PCR

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

The generation of characteristic signatures from virtually any nucleic acid, even of anonymous nature, has been made possible by the invention of nucleic acid scanning (Livak et al. 1992; Bassam et al. 1995). These signatures are composed of arbitrary collections of amplification products that result from the targeting of a multiplicity of anonymous sites (amplicons) in template DNA or RNA molecules. The targeted sites are amplified with one or more oligodeoxynucleotides to produce arbitrary but entirely characteristic “fingerprint” patterns. Nucleic acid scanning allows detection of polymorphic DNA or RNA without prior knowledge of sequence or cloned and characterized probes.

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Authors
  1. G. Caetano-Anollés
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Editors and Affiliations

  1. Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Via Pascoli, 06123, Perugia, Italia

    Maria Rita Micheli

  2. Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma “Tor Vergata”, Via Tor Vergata, 00133, Roma, Italia

    Rodolfo Bova

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© 1997 Springer-Verlag Berlin Heidelberg

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Caetano-Anollés, G. (1997). DNA Amplification Fingerprinting. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_10

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  • DOI: https://doi.org/10.1007/978-3-642-60441-6_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-47812-3

  • Online ISBN: 978-3-642-60441-6

  • eBook Packages: Springer Book Archive

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