Abstract
A significant advantage in DNA fingerprinting technology was made in the early 1990s through the development of a novel polymerase chain reaction (PRC)-based strategy which involves the use of a single oligonucleotide primers of arbitrary sequence to amplifly random genomic DNA fragments (Welsh and McClelland 1990; Williams et al. 1990; Caetano-Anollés et al. 1991). This strategy, often referred to as arbitrarily primed PCR, allows detection of polymorphisms between individuals (or strains) as differences between the patterns of DNA fragments amplified from the different DNAs using a given primer.
Keywords
- Primer Binding Site
- Arbitrary Primer
- Random Amplification
- Arbitrary Oligonucleotide
- Random Polymerase Chain Reaction
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Birkenmeier EH, Schneider U, Thurston SJ (1992) Fingerprinting genomes by use of PCR primers that encode protein motifs or contain sequences that regulate gene expression. Mamm Genome 2:537–545
Caetano-Anollés G, Gresshoff PM (1994) DNA amplification fingerprinting using arbitrary mini-hairpin oligonucleotide primers. Bio/Technology 12:619–623
Caetano-Anollés G, Gresshoff PM (1996) Generation of sequence signatures from DNA amplification fingerprints with mini-hairpin and microsatellite primers. BioTechniques 20, in press
Caetano-Anollés G, Bassam BJ, Gresshoff PM (1991) DNA amplification fingerprint- ing using very short arbitrary oligonucleotide primers. Bio/Technology 9:553–557
Caetano-Anollés G, Bassam BJ, Gresshoff PM (1992a) Primer-template interactions during DNA amplification fingerprinting with single arbitrary oligonucleotides. Mol Gen Genet 235:157–165
Caetano-Anollés G, Bassam BJ, Gresshoff PM (1992b) DNA fingerprinting: MAAPing out a RAPD redefinition? Biotechnology 10:937
Caetano-Anollés G, Bassam BJ, Gresshoff PM (1993) Enhanced detection of polymorphic DNA by multiple arbitrary amplicon profiling of endonuclease digested DNA: identification of markers linked to the supernodulation locus in soybean. Mol Gen Genet 241:57–64
Cifarelli RA, Gallitelli M, Cellini F (1995) Random amplified hybridization mycrosatellites (RAHM): isolation of a new class of microsatellite-containing DNA clones. Nucleic Acids Res 23:3802–3803
Gang DR, Weber DJ (1995) Preparation of genomic DNA for RAPD analysis from thick-walled dormant teliospores of Tilletia species. Bio Technique 19:92–97
Guidet F (1994) A powerful new techique to quickly prepare hundreds of plant extracts for PCR and RAPD analyses. Nucleic Acids Res 22:1772–1773
Ionov Y, Peinado MA, Malkhosyan S, Shibata D, Perucho M (1993) Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis. Nature 363:558–561
Kohno T, Moroshita K, Takano H, Shapiro DN, Yokota J (1994) Homozygous deletion at chromosome 2q33 in human small-cell lung carcinoma identified by arbitrarily primed PCR genomic fingerprinting. Oncogene 9:103–108
Landegren U, Kaiser R, Sanders J, Hood L (1988) A ligase-mediated gene detection technique. Science 241:1077–1080
Matioli SR, de Brito R (1995) Obtaining genetic markers by using double-stringency PCR with microsatellites and arbitrary primers. Bio Technique 19:752–756
McClelland M, Welsh J (1994) DNA fingerprinting by arbitrarily primed PCR. PCR Methods Applic 4:s59-s65
McClelland M, Petersen C, Welsh J (1992) Length polymorphisms in tRNA intergenic spacers detected using the polymerase chain reaction can distinguish streptococcal strains and species. J Clin Microbiol 30:1499–1504
McClelland M, Arensdorf H, Cheng R, Welsh J (1994) Arbitrarily primed PCR fingerprints resolved on SSCP gels. Nucleic Acids Res 22:1770–1771
McClelland M, Mathieu-Daude F, Welsh J (1995) RNA fingerprinting and differential display using arbitrarily primed PCR. Trends Genet 11:242–246
Micheli MR, Bova R, Calissano P, D’Ambrosio E (1993) Randomly amplified polymorphic DNA fingerprinting using combinations of oligonucleotide primers. Bio Techniques 15:388–390
Micheli MR, Bova R, Pascale E, D’Ambrosio E (1994) Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method. Nucleic Acids Res 22:1921–1922
Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993
Peinado MA, Malkhosyan S, Velazquez A, Perucho M (1992) Isolation and characterization of allelic losses and gains in colorectal tumors by arbitrarily primed polymerase chain reaction. Proc Natl Acad Sci USA 89:10065–10069
Richardson T, Cato S, Ramser J, Kahl G, Weising K (1995) Hybridization of microsatellites to RAPD: a new source of polymorphic markers. Nucleic Acids Res 23:3798–3799
Sakallah SA, Lanning RW, Cooper DL (1995) DNA fingerprinting of crude bacterial lysates using degenerate RAPD primers. PCR Methods Applic 4:265–268
Schlegel J, Vogt T, Münkel K, Rüschoff J (1996) DNA fingerprinting of mammalian cell lines using nonradioactive arbitrarily primed PCR (AP-PCR). Bio Techniques 20:178–180
Steiner JJ, Poklemba CJ, Fjellstrom RG, Elliott LF (1995) A rapid one-tube genomic DNA extraction process for PCR and RAPD analysis. Nucleic Acids Res 23:2569–2570
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Welsh J, McClelland M (1991) Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers. Nucleic Acids Res 19:5275–5279
Welsh J, McClelland M (1992) PCR-amplified length polymorphisms in tRNA intergenic spacers for categorizing staphylococci. Mol Microbiol 6:1673–1680
Welsh J, Petersen C, McClelland M (1991) Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucleic Acids Res 19:303–306
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymor- phisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Williams JGK, Hanafey MK, Rafalski JA, Tingey SV (1993) Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol 218:704–740
Wu DY, Ugozzoli L, Pal BK, Wallace RB (1989) Allele-specific enzymatic amplification of β-globin genomic DNA for diagnosis of sikle cell anemia. Proc Natl Acad Sci USA 86:2757–2760
Zhu H, Qu F, Zhu L (1993) Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Res 21:5279–5280
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© 1997 Springer-Verlag Berlin Heidelberg
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Micheli, M.R., Bova, R. (1997). Overview. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_1
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DOI: https://doi.org/10.1007/978-3-642-60441-6_1
Publisher Name: Springer, Berlin, Heidelberg
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