Abstract
In mammalian DNA, 3%–5% of cytosine residues are present as 5-methylcytosine (Holiday and Grigg 1993). This methylation is involved in several epigenetic processes, including genomic imprinting and gene expression (Tilghman 1993). DNA hypomethylation is one of the possible causes and one of the consequences of mammalian neoplasia and also occurs in cultured cells after treatment with certain carcinogenic drugs (Spruck et al. 1993). Changes in DNA methylation can affect DNA conformation (Bird 1992).
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References
Alonso A, Breuer B, Steuer B, Fischer J (1991) The F9-EC cell line as a model for the analysis of differentiation. Int J Dev Biol 35 (4): 389–397
Amacher D, Turner G (1987) The mutagenicity of 5-azacytidine and other inhibitors of replication DNA synthesis in the L5178Y mouse lymphoma cell. Mutat Res 176: 123–131
Bird A (1992) The essentials of DNA-methylation. Cell 70: 5–8
Counts JL, Goodman JI (1994) Hypomethylation of DNA: an epigenetic mechanism involved in tumor promotion. Mol Carcinog 11: 185–188
Downes CS, Mullinger AM, Johnson RT (1991) Inhibitors of topoisomerase II prevent chromatid separation in mammalian cells but do not prevent exit from mitosis. Proc Natl Acad Sci USA 88: 8895–8899
Gaulden ME (1987) Hypothesis: some mutagens directly alter specific chromosomal proteins (DNA topoisomerase II and peripheral proteins) to produce chromosome stickiness, which causes chromosome aberrations. Mutagenesis 2: 357–365
Greger V, Debus N, Lohmann D, Hopping W, Passarge E, Horsthemke B (1994) Frequency and parental origin of hypermethylated RBI alleles in retinoblastoma. Hum Genet 94: 491–496
Holiday R, Grigg GW (1993) DNA-methylation and mutation. Mutat Res 285: 61–67
Keshet I, Lieman-Hurwitz J, Cedar H (1986) DNA-methylation affects the formation of active chromatin. Cell 44: 535–545
Kirchner S, Stopper H, Papp T, Eckert I, Yoo HJ, Vig BK, Schiffmann D (1993) Cytogenetic changes in primary, immortalized and malignant mammalian cells. Toxicol Lett 67: 283–295
Kirchner S, Schiffmann D, Stopper H (1995) The influence of DNA-methylation on topoisomerase II activity and genomic instability. Toxicol In Vitro (in press)
Lewis J, Bird A (1991) DNA-methylation and chromatin structure. FEBS Lett 285: 155–159
Makos M, Nelkin BD, Reiter RE, Gnarra JR, Brooks J, Isaacs W, Linehan M, Baylin SB (1993) Regional DNA-hypermethylation at D17S5 precedes 17p structural changes in the progression of renal tumors. Cancer Res 53: 2719–2722
McGregor DB, Brown AG, Cattanach P, Shepherd W, Riach C, Daston DS, Caspary WJ (1989) TFT and 6TG resistance of mouse lymphoma cells to analogs of azacy-tidine. Carcinogenesis 10: 2003–2008
Razin A, Cedar H (1977) Distribution of 5-methy Icy tosine in chromatin. Proc Natl Acad Sci USA 74: 2725–2728
Razin A, Szyf M, Kafri T, Roll M, Giloh H, Scarpa S, Carotti D, Cantoni GL (1986) Replacement of 5-methylcytosine by cytosine: a possible mechanism for transient DNA-demethylation during differentiation. Proc Natl Acad Sci USA 83: 2827–2831
Spencer DL, Hines KC, Caspary WJ (1994) An in situ protocol for measuring the expression of chemically induced mutations in mammalian cells. Mutat Res 312: 85–98
Spruck CH III, Rideout WM III, Jones PA (1993) DNA-methylation and cancer. In: Jost JP, Saluz HP (eds) DNA-methylation: molecular biology and biological significance. Birkhäuser Basel, pp 487–509
Stopper H, Körber C, Schiffmann D, Caspary WJ (1993) Cell cycle dependent micro- nucleus formation and mitotic disturbances in mammalian cells treated with 5-aza-cytidine. Mutat Res 300: 165–177
Stopper H, Eckert I, Schiffmann D, Spencer DL, Caspary WJ (1994) Is micronucleus induction by aneugens an early event leading to mutagenesis? Mutagenesis 9 (5): 411–416
Teubner B, Schulz W (1994) Exemption of satellite DNA from demethylation in immortalized derivatives of F9 mouse embryonal carcinoma cells. Exp Cell Res 210: 192–200
Tilghman S (1993) DNA-methylation: a phoenix rises. Proc Natl Acad Sci USA 90: 8761–8762
Wong AKC, Rattner JB (1988) Sequence organization and cytological localization of the minor satellite of mouse. Nucleic Acid Res 16 (24): 11645–11661
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Stopper, H., Eckert, I., Wagener, P., Schulz, W.A. (1997). Formation of Micronuclei and Inhibition of Topoisomerase II in the Comet Assay in Mammalian Cells with Altered DNA Methylation. In: Müller-Hermelink, H.K., Neumann, HG., Dekant, W. (eds) Risk and Progression Factors in Carcinogenesis. Recent Results in Cancer Research, vol 143. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60393-8_12
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DOI: https://doi.org/10.1007/978-3-642-60393-8_12
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