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Autologouos Transplantation of Urothelium into Demucosalized Gastrointestinal Segments

  • Conference paper
Book cover Biological Matrices and Tissue Reconstruction

Abstract

The use of demucosalized gastrointestinal segments for bladder augmentation is limited by shrinkage and fibrosis occurring during the reepithelialization process. Shrinkage and fibrosis was shown to be reduced by early and complete urothelial coverage of the gastrointestinal segment. Transplantation of in vitro expanded autologous urothelial cells was suggested to achieve rapid epithelial coverage of gastrointestinal segments. We have shown that complete reepithelialization as well as differentiation of in vitro expanded urothelial cells can be achieved in vivo as early as two weeks after transplantation onto demucosalized gastrointestinal segments. For transplantation we used cells grown on collagen type I membranes, hyaluronic acid membranes, or urothelial cells incorporated into fibrin gels. Most successful with respect to complete reepithelialization and differentiation was the transfer of urothelial cells grown on collagen type I membranes. However, shrinkage and fibrosis of the gastrointestinal segments were not prevented by the rather rapid reepithelialization achieved with urothelial cells grown on collagen membranes. We are currently investigating whether transplantation of an already differentiated multilayered urothelium or distension of gastrointestinal segments prevents shrinkage and fibrosis.

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Authors
  1. B. M. Schaeffer
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  2. W. Back
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  3. M. D. Kramer
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  4. C. Schober
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  5. K. L. Waag
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  6. C. Lorenz
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Editors and Affiliations

  1. Abt. Plastische und Handchirurgie, Chirurgische Universitätsklinik Freiburg, Hugstetter Straße 55, D-79106, Freiburg, Germany

    G. Björn Stark , Raymund Horch  & Eszter TÁczos ,  & 

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© 1988 Springer-Verlag Berlin Heidelberg

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Schaeffer, B.M., Back, W., Kramer, M.D., Schober, C., Waag, K.L., Lorenz, C. (1988). Autologouos Transplantation of Urothelium into Demucosalized Gastrointestinal Segments. In: Stark, G.B., Horch, R., TÁczos, E. (eds) Biological Matrices and Tissue Reconstruction. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60309-9_15

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  • DOI: https://doi.org/10.1007/978-3-642-60309-9_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64347-7

  • Online ISBN: 978-3-642-60309-9

  • eBook Packages: Springer Book Archive

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