Abstract
Since the production of the first genetically engineered plants in the middle 1980s (Horch et al. 1985), there has been an explosion of interest in the production of transgenic plants for both basic and applied work. Large efforts have been placed in the development and refinement of methods for transgenic plant production and also in the isolation and characterization of useful genes for introduction into plants. Through gene transfer, plants have already been produced that contain genes for disease and insect resistance, and modified fruit and grain quality. Many of the products of plant biotechnology would not exist today without particle bombardment gene transfer methods. Using Biolistics, transformation of many plants and tissues that had been recalcitrant to manipulation, has become much more routine. Particle bombardment or Biolistics has been used to transform a large number of different plant species (reviewed by Christou 1994), as well as some animals, fungi, and bacteria.
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Keywords
- Transgenic Plant
- Particle Bombardment
- Selectable Marker Gene
- Shoot Meristem
- Chloramphenicol Acetyl Transferase
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Finer, J.J., Finer, K.R., Ponappa, T. (2000). Particle Bombardment Mediated Transformation. In: Hammond, J., McGarvey, P., Yusibov, V. (eds) Plant Biotechnology. Current Topics in Microbiology and Immunology, vol 240. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60234-4_3
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