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Combinatorial Chemistry in Biology pp 37–54Cite as

The Two Hybrid Toolbox

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 243))

Abstract

Protein—protein interactions are required for nearly every cellular function and are therefore universally studied in biology. Traditionally such interactions have been identified and characterized by the means of biochemistry, and the power of this approach is certainly undisputed. However, besides the fact that in the “old days” the composition of protein complexes was mostly studied in vitro, the arsenal of biochemical methods is very effective for tight interactions only, especially those with slow off-rate kinetics. Protein purification protocols have substantial time requirements, which means that interactions of interest might simply not survive the isolation procedure. Chemical cross-linking, i.e. covalent tethering of proteins, can sometimes overcome this limitation but this is not a widely used method. This is partly due to the fact that specific cross-linking of proteins may not be feasible under relevant conditions, e.g. inside a cell.

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Authors and Affiliations

  1. Laboratorium für Molekulare Biologie, Genzentrum der Universität München, Feodor-Lynen Str. 25, D-81377, Munich, Germany

    W. Kolanus

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  1. W. Kolanus
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Editors and Affiliations

  1. Kekule-Institut für Organische Chemie und Biochemie, Gerhard-Domagk-Str. 1, D-53121, Bonn, Germany

    Michael Famulok

  2. Institut für Biochemie — Genzentrum, Ludwig Maximilians Universität München, Feodor-Lynnen-Str. 25, D-81377, München, Germany

    Ernst-L. Winnacker

  3. The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Chi-Huey Wong

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© 1999 Springer-Verlag Berlin Heidelberg

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Kolanus, W. (1999). The Two Hybrid Toolbox. In: Famulok, M., Winnacker, EL., Wong, CH. (eds) Combinatorial Chemistry in Biology. Current Topics in Microbiology and Immunology, vol 243. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60142-2_3

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  • DOI: https://doi.org/10.1007/978-3-642-60142-2_3

  • Publisher Name: Springer, Berlin, Heidelberg

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