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Antigen Sequence- and Library-Based Mapping of Linear and Discontinuous Protein—Protein-Interaction Sites by Spot Synthesis

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Combinatorial Chemistry in Biology

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 243))

Abstract

Peptides synthesized on continuous cellulose membranes by the spot synthesis technique (Frank et al. 1992) have been increasingly used to study molecular recognition events. The application of these positionally addressable peptide libraries include investigating protein/protein (literature cited throughout the text), protein/DNA (Kramer et al. 1993; Reuter et al. 1998) and protein/metal interactions (Malin et al. 1995). In addition, substrate specificities of kinases (Toomik et al. 1996; Tegge et al. 1998; Mukhija et al. 1998), proteases (Duan and Laursen 1994; Kramer et al. 1998; Reineke et al. 1999) and chaperones (Rüdiger et al. 1997) have been determined. In this chapter we review protein sequence- and library-based approaches to map linear and discontinuous antibody/antigen and receptor/ligand contact sites using the spot synthesis technique.

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Author notes

  1. U. Reineke

    Present address: Jerini Bio Tools GmbH, Rudower Chaussee 29, D-12489, Berlin, Germany

Authors and Affiliations

  1. Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, D-10117, Berlin, Germany

    A. Kramer & J. Schneider-Mergener

Authors
  1. U. Reineke
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  2. A. Kramer
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  3. J. Schneider-Mergener
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Editor information

Editors and Affiliations

  1. Kekule-Institut für Organische Chemie und Biochemie, Gerhard-Domagk-Str. 1, D-53121, Bonn, Germany

    Michael Famulok

  2. Institut für Biochemie — Genzentrum, Ludwig Maximilians Universität München, Feodor-Lynnen-Str. 25, D-81377, München, Germany

    Ernst-L. Winnacker

  3. The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Chi-Huey Wong

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© 1999 Springer-Verlag Berlin Heidelberg

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Reineke, U., Kramer, A., Schneider-Mergener, J. (1999). Antigen Sequence- and Library-Based Mapping of Linear and Discontinuous Protein—Protein-Interaction Sites by Spot Synthesis. In: Famulok, M., Winnacker, EL., Wong, CH. (eds) Combinatorial Chemistry in Biology. Current Topics in Microbiology and Immunology, vol 243. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60142-2_2

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  • DOI: https://doi.org/10.1007/978-3-642-60142-2_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64274-6

  • Online ISBN: 978-3-642-60142-2

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