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High Resolution 3-D Imaging of Living Cells by Image Restoration

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Imaging Living Cells

Part of the book series: Springer Lab Manual ((SLM))

Abstract

In this chapter we present an approach to high spatial and temporal resolution three-dimensional (3-D) fluorescence imaging of living cells. This approach integrates sensitive CCD cameras, wide field epifluorescence microscopes and a computational method, image restoration, to obtain 3-D images with a lateral resolution of 100 nanometers, which is better than the resolution that can be achieved by either confocal fluorescence microscopy or conventional epifluorescence microscopy without image restoration. The optical and detector efficiency of the system allows us to obtain a time series of many 3-D images from the same cell with minimal photobleaching. When combined with the high speed camera and rapid focus drive described in figure 1, a 3-D image of a cell can be acquired in only 24 milliseconds.

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Author information

Authors and Affiliations

  1. Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, MA, 01605, USA

    Richard A. Tuft

Authors
  1. Walter A. Carrington
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  2. Kevin E. Fogarty
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  3. Lawrence M. Lifshitz
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  4. Richard A. Tuft
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Editor information

Editors and Affiliations

  1. Department of Experimental and Diagnostic Medicine, Section of General Pathology, Via Borsari 46, 44100, Ferrara, Italy

    Rosario Rizzuto M.D., Ph. D.

  2. Department of Biomedical Sciences and CNR Center for the Study of Biomembranes, University of Padova, Via G. Colombo 3, 35121, Padova, Italy

    Cristina Fasolato Ph. D.

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© 1999 Springer-Verlag Berlin Heidelberg

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Carrington, W.A., Fogarty, K.E., Lifshitz, L.M., Tuft, R.A. (1999). High Resolution 3-D Imaging of Living Cells by Image Restoration. In: Rizzuto, R., Fasolato, C. (eds) Imaging Living Cells. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60003-6_2

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  • DOI: https://doi.org/10.1007/978-3-642-60003-6_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-65051-5

  • Online ISBN: 978-3-642-60003-6

  • eBook Packages: Springer Book Archive

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